公开(公告)号：US11427847B2

公开(公告)日：2022-08-30

申请号：US17163033

申请日：2021-01-29

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US11078508B2

公开(公告)日：2021-08-03

申请号：US16211632

申请日：2018-12-06

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12P21/02 ,  C12N9/58 ,  C12N15/80 ,  C12N15/04

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20210155967A1

公开(公告)日：2021-05-27

申请号：US17163033

申请日：2021-01-29

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US11078510B2

公开(公告)日：2021-08-03

申请号：US16996168

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US10400260B2

公开(公告)日：2019-09-03

申请号：US14663118

申请日：2015-03-19

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12N9/58 ,  C12P21/00 ,  C12N15/80 ,  C12P21/02

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US11078509B2

公开(公告)日：2021-08-03

申请号：US16996159

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US11001869B2

公开(公告)日：2021-05-11

申请号：US16995820

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20200377921A1

公开(公告)日：2020-12-03

申请号：US16995820

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20190093143A1

公开(公告)日：2019-03-28

申请号：US16211632

申请日：2018-12-06

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/80 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20150225761A1

公开(公告)日：2015-08-13

申请号：US14663118

申请日：2015-03-19

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80

CPC classification number: C12P21/00 ,  C12N9/58 ,  C12N15/80 ,  C12P21/02

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

Abstract translation: 本发明涉及母体木霉菌株的突变体，其包含编码多肽的多核苷酸和选自第一枯草杆菌蛋白酶样丝氨酸蛋白酶基因，第一天冬氨酸蛋白酶基因，胰蛋白酶抑制蛋白酶基因的一个或多个（若干）基因， 如丝氨酸蛋白酶基因，第二枯草杆菌蛋白酶样丝氨酸蛋白酶基因和第二天冬氨酸蛋白酶基因，其中所述一个或多个（若干）基因被修饰，使得突变菌株缺乏产生一种或多种（若干）选自 与母本木霉属菌株相比，分别在相同的条件下培养时由第一枯草杆菌蛋白酶样丝氨酸蛋白酶，第一天冬氨酸蛋白酶，胰蛋白酶样丝氨酸蛋白酶，第二枯草杆菌蛋白酶样丝氨酸蛋白酶和第二天冬氨酸蛋白酶组成的组 条件。 本发明还涉及在这种突变体中产生多肽的方法和用于产生这种突变体的方法。