公开(公告)号：US11279921B2

公开(公告)日：2022-03-22

申请号：US16603639

申请日：2018-04-10

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Noriko Tsutsumi ,  Keiichi Ayabe ,  Zhengfang Kang

IPC: C12N9/34 ,  C12P19/02 ,  C12P19/14

Abstract: The present invention relates to glucoamylase variants having an increase in raw starch activity compared to the guy-coamylase disclosed as SEQ ID NO: 2, comprising one or more modifications in the catalytic domain and/or one or more modifications in the starch binding domain selected from: a) at least one, preferably at least two, preferably at least three, preferably at least four of: V18M, T43K, N112L, T116R, A117Q, G120S, A271F, Y295W, Q318Y; and/or b) Introducing at least three, preferably at least four substitutions selected from the group: S458C, S458SCGG, S458SGGC, A493V, A518K, E520Q, N527M, S540K, R, S(G)546P, T(V)549W, N503R, N539R+I541Y+T543V+A545S+S546GCGV+G547S+S548T+T549A. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10538752B2

公开(公告)日：2020-01-21

申请号：US15679805

申请日：2017-08-17

Applicant: NOVOZYMES A/S

Inventor： Guillermo Coward-Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/26 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/30 ,  C12G3/02 ,  C12P19/14 ,  C12P21/02 ,  C12P7/06 ,  C12C7/04 ,  C12C5/00

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

公开(公告)号：US20170349887A1

公开(公告)日：2017-12-07

申请号：US15679805

申请日：2017-08-17

Applicant: NOVOZYMES A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12C7/04 ,  C12G3/02 ,  C12P19/14 ,  C12C5/00 ,  C12N9/26 ,  C12P7/06 ,  C12P21/02

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

公开(公告)号：US20150337277A1

公开(公告)日：2015-11-26

申请号：US14653154

申请日：2013-12-13

Applicant: Novozymes A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/30 ,  C12G3/02 ,  C12P7/06

CPC classification number: C12N9/242 ,  C12C5/004 ,  C12C7/04 ,  C12G3/02 ,  C12N9/2414 ,  C12P7/06 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01001 ,  Y02E50/17

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

Abstract translation: 本公开涉及具有α-淀粉酶活性的分离的多肽，编码多肽的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生多肽的方法，以及使用多肽的方法，包括在乙醇生产过程 。

公开(公告)号：US12269852B2

公开(公告)日：2025-04-08

申请号：US17936076

申请日：2022-09-28

Applicant: NOVOZYMES A/S

Inventor： Marc Dominique Morant ,  Tomoko Matsui ,  Kirk Matthew Schnorr ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Zhengfang Kang

IPC: C12P7/06 ,  C07K14/39 ,  C07K14/395 ,  C12N9/24 ,  C12N9/26 ,  C12N15/81 ,  C12P19/14

Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.

公开(公告)号：US11220679B2

公开(公告)日：2022-01-11

申请号：US16635195

申请日：2018-08-06

Applicant: NOVOZYMES A/S

Inventor： Noriko Tsutsumi ,  Tomoko Matsui ,  Yuma Kurakata

IPC: C12N9/24 ,  C12P19/14

Abstract: The present invention relates to trehalase variants of the Myceliophthora sepedonium GH37 trehalase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US09909112B2

公开(公告)日：2018-03-06

申请号：US15587576

申请日：2017-05-05

Applicant: NOVOZYMES A/S

Inventor： Ming Li ,  Junxin Duan ,  Noriko Tsutsumi ,  Guillermo Coward-Kelly ,  Henrik Lundkvist

IPC: C12N9/30 ,  C12N1/00 ,  C12N1/15 ,  C12N1/19 ,  C12P7/14 ,  A21D8/04

CPC classification number: C12N9/242 ,  A21D8/042 ,  C12Y302/01001

Abstract: The present invention relates to isolated polypeptides having alpha-amylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.

公开(公告)号：US09765317B2

公开(公告)日：2017-09-19

申请号：US14653154

申请日：2013-12-13

Applicant: Novozymes A/S

Inventor： Guillermo Coward Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/26 ,  C12N9/30 ,  C12G3/02 ,  C12P19/14 ,  C12P21/02 ,  C12P7/06 ,  C12C7/04 ,  C12C5/00

CPC classification number: C12N9/242 ,  C12C5/004 ,  C12C7/04 ,  C12G3/02 ,  C12N9/2414 ,  C12P7/06 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01001 ,  Y02E50/17

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.

公开(公告)号：US20160152960A1

公开(公告)日：2016-06-02

申请号：US14787418

申请日：2014-04-29

Applicant: NOVOZYMES A/S

Inventor： Noriko Tsutsumi ,  Keiichi Ayabe ,  Siik Kishishita

IPC: C12N9/34 ,  C12P19/20 ,  C12P19/02 ,  A23L1/23

Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及葡糖淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US20210087543A1

公开(公告)日：2021-03-25

申请号：US16635195

申请日：2018-08-06

Applicant: NOVOZYMES A/S

Inventor： Noriko Tsutsumi ,  Tomoko Matsui ,  Yuma Kurakata

IPC: C12N9/24 ,  C12P19/14

Abstract: The present invention relates to trehalase variants of the Myceliophthora sepedonium GH37 trehalase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.