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公开(公告)号：US20120272402A1
公开(公告)日：2012-10-25
申请号：US13534231
申请日：2012-06-27
申请人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
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公开(公告)号：US08232456B2
公开(公告)日：2012-07-31
申请号：US12301824
申请日：2007-05-24
申请人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids from event MIR162. The invention also relates to assays for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion sites. The invention further relates to corn plants comprising the genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及事件MIR162的核酸。 本发明还涉及基于导入MIR162事件的插入到玉米基因组中的重组构建体的DNA序列和在插入位点侧翼的基因组序列来检测MIR162事件的存在的测定。 本发明还涉及包含MIR162的基因型的玉米植物和通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。
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公开(公告)号：US20120167260A1
公开(公告)日：2012-06-28
申请号：US13421920
申请日：2012-03-16
申请人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
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公开(公告)号：US20120264130A1
公开(公告)日：2012-10-18
申请号：US13534202
申请日：2012-06-27
申请人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及对事件MIR162独特的核酸和基于插入到导致MIR162事件的玉米基因组中的重组构建体的DNA序列以及侧翼于插入位点的基因组序列的检测MIR162事件的存在的方法 。 本发明进一步涉及包含MIR162的转基因基因型的玉米植物，以及通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。
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公开(公告)号：US20090300784A1
公开(公告)日：2009-12-03
申请号：US12301824
申请日：2007-05-24
申请人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeff Bottoms , Moez Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids from event MIR162. The invention also relates to assays for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion sites. The invention further relates to corn plants comprising the genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及事件MIR162的核酸。 本发明还涉及基于导入MIR162事件的插入到玉米基因组中的重组构建体的DNA序列和在插入位点侧翼的基因组序列来检测MIR162事件的存在的测定。 本发明还涉及包含MIR162的基因型的玉米植物和通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。
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公开(公告)号：US08618272B2
公开(公告)日：2013-12-31
申请号：US13534202
申请日：2012-06-27
申请人： Nykoll Long , Jeffrey Bottoms , Moez Rajabali Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeffrey Bottoms , Moez Rajabali Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及对事件MIR162独特的核酸和基于插入到导致MIR162事件的玉米基因组中的重组构建体的DNA序列以及侧翼于插入位点的基因组序列的MIR162事件的存在的方法 。 本发明进一步涉及包含MIR162的转基因基因型的玉米植物，以及通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。
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公开(公告)号：US08455720B2
公开(公告)日：2013-06-04
申请号：US13421920
申请日：2012-03-16
申请人： Nykoll Long , Jeffrey Bottoms , Moez Rajabali Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
发明人： Nykoll Long , Jeffrey Bottoms , Moez Rajabali Meghji , Hope Hart , Qiudeng Que , Derrick Pulliam
IPC分类号： C12N15/82
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8286 , C12Q2600/13 , Y02A40/162
摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
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公开(公告)号：US08399254B2
公开(公告)日：2013-03-19
申请号：US13030809
申请日：2011-02-18
申请人： Qiudeng Que
发明人： Qiudeng Que
CPC分类号： C12N15/8213
摘要： Host cells comprise a site for targeted integration and iterative stacking of DNA through recombination. The site comprises a target sequence comprising (a) a truncated functional sequence effective for restoration of a functional sequence when operably linked through a recombination event with a sequence that completes the truncated functional sequence; (b) a first recombinase recognition site derived through site-specific recombination-mediated excision of nucleotide sequences flanked by recombinase recognition sequences; and (c) a host homology sequence located between the truncated functional sequence and the first recombinase recognition site derived through site-specific recombination-mediated excision of nucleotide sequences flanked by recombinase recognition sequences.
摘要翻译： 宿主细胞包含用于靶向整合的位点并通过重组迭代堆叠DNA。 该位点包括靶序列，其包含（a）当通过重组事件与完成截短的功能序列的序列可操作地连接时，有效恢复功能性序列的截短的功能序列; （b）通过位点特异性重组介导的切除重组酶识别序列侧翼的核苷酸序列衍生的第一重组酶识别位点; 并且（c）位于截短的功能序列和第一重组酶识别位点之间的宿主同源性序列，其通过位点特异性重组介导的切除重组酶识别序列侧翼的核苷酸序列而衍生。
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公开(公告)号：US20090023212A1
公开(公告)日：2009-01-22
申请号：US11880107
申请日：2007-07-19
申请人： Heng Zhong , Qiudeng Que
发明人： Heng Zhong , Qiudeng Que
CPC分类号： C12N15/8205
摘要： The present disclosure provides methods for the transformation of soybean cells or tissue and regeneration of the soybean cells or tissue into transformed plants. The disclosed methods utilize an explant prepared from an immature soybean inflorescence, shoot apex, leaf axillary, shoot axillary, or combination thereof which can be induced directly to form shoots that give rise to transgenic plants via organogenesis.
摘要翻译： 本公开提供了用于转化大豆细胞或组织以及将大豆细胞或组织再生成转化植物的方法。 所公开的方法利用由未成熟的大豆花序，茎尖，叶腋生，芽腋生或其组合制备的外植体，其可以直接诱导形成通过器官发生产生转基因植物的芽。
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10.发明申请公开(公告)号：US20110191914A1
公开(公告)日：2011-08-04
申请号：US13030809
申请日：2011-02-18
申请人： Qiudeng Que
发明人： Qiudeng Que
CPC分类号： C12N15/8213
摘要： The invention provides various methods for the targeted integration and stacking of nucleotide sequences in the genome of a host plant cell using homologous recombination.
摘要翻译： 本发明提供用于使用同源重组在宿主植物细胞的基因组中靶向整合和堆叠核苷酸序列的各种方法。
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