公开(公告)号：US08618272B2

公开(公告)日：2013-12-31

申请号：US13534202

申请日：2012-06-27

申请人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/11 ,  C12N15/32

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及对事件MIR162独特的核酸和基于插入到导致MIR162事件的玉米基因组中的重组构建体的DNA序列以及侧翼于插入位点的基因组序列的MIR162事件的存在的方法 。 本发明进一步涉及包含MIR162的转基因基因型的玉米植物，以及通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。

公开(公告)号：US08455720B2

公开(公告)日：2013-06-04

申请号：US13421920

申请日：2012-03-16

申请人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeffrey Bottoms ,  Moez Rajabali Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/82

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

公开(公告)号：US20120272402A1

公开(公告)日：2012-10-25

申请号：US13534231

申请日：2012-06-27

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/82 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

公开(公告)号：US08232456B2

公开(公告)日：2012-07-31

申请号：US12301824

申请日：2007-05-24

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids from event MIR162. The invention also relates to assays for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion sites. The invention further relates to corn plants comprising the genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及事件MIR162的核酸。 本发明还涉及基于导入MIR162事件的插入到玉米基因组中的重组构建体的DNA序列和在插入位点侧翼的基因组序列来检测MIR162事件的存在的测定。 本发明还涉及包含MIR162的基因型的玉米植物和通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。

公开(公告)号：US20120167260A1

公开(公告)日：2012-06-28

申请号：US13421920

申请日：2012-03-16

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： A01C1/06 ,  A01H5/00

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

公开(公告)号：US20120264130A1

公开(公告)日：2012-10-18

申请号：US13534202

申请日：2012-06-27

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： C12N15/09 ,  C12N15/63 ,  C07K14/00 ,  C12Q1/68 ,  C07H21/00

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及对事件MIR162独特的核酸和基于插入到导致MIR162事件的玉米基因组中的重组构建体的DNA序列以及侧翼于插入位点的基因组序列的检测MIR162事件的存在的方法 。 本发明进一步涉及包含MIR162的转基因基因型的玉米植物，以及通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。

公开(公告)号：US20090300784A1

公开(公告)日：2009-12-03

申请号：US12301824

申请日：2007-05-24

申请人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

发明人： Nykoll Long ,  Jeff Bottoms ,  Moez Meghji ,  Hope Hart ,  Qiudeng Que ,  Derrick Pulliam

IPC分类号： A01H1/00 ,  C12N15/11 ,  C12Q1/68 ,  C12N5/04 ,  A01H5/00 ,  C07K14/415 ,  C12N15/82

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/8286 ,  C12Q2600/13 ,  Y02A40/162

摘要： A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids from event MIR162. The invention also relates to assays for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion sites. The invention further relates to corn plants comprising the genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

摘要翻译： 公开了一种称为MIR162的新型转基因玉米事件。 本发明涉及事件MIR162的核酸。 本发明还涉及基于导入MIR162事件的插入到玉米基因组中的重组构建体的DNA序列和在插入位点侧翼的基因组序列来检测MIR162事件的存在的测定。 本发明还涉及包含MIR162的基因型的玉米植物和通过将包含MIR162基因型的玉米植物与其本身或另一种玉米品种杂交来生产玉米植物的方法。 包含MIR162基因型的玉米植物种子也是本发明的目的。 本发明还涉及使用MIR162玉米植物控制昆虫的方法。

公开(公告)号：US09096896B2

公开(公告)日：2015-08-04

申请号：US13378869

申请日：2010-06-18

申请人： Hope Hart

发明人： Hope Hart

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/137

摘要： Improved methods of quantifying nucleic acids that are unique to a transgenic corn event designated Bt11 in a biological sample and compositions thereof are disclosed. The invention further relates to primer pairs used in the method that are unique to event Bt11.

摘要翻译： 公开了一种定量生物样品中命名为Bt11的转基因玉米事件独特的核酸的改进方法及其组合物。 本发明还涉及用于事件Bt11的方法中使用的引物对。

公开(公告)号：US08309516B2

公开(公告)日：2012-11-13

申请号：US12529246

申请日：2008-03-26

申请人： Hope Hart ,  Jeng S. Chen ,  Cheryl Stacy ,  Frederick Walters

发明人： Hope Hart ,  Jeng S. Chen ,  Cheryl Stacy ,  Frederick Walters

IPC分类号： C07K14/325

CPC分类号： C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins (eHIPs) having toxicity to at least corn rootworm are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different Bacillus thuringiensis (Bt) Cry proteins or a modified Cry proteins an eHIP having activity against corn rootworm is designed. Nucleic acid molecules encoding the novel eHIPs are also provided. Methods of making the eHIPs and methods of using the eHIPs and nucleic acids encoding the eHIPs of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.

摘要翻译： 公开了用于控制植物害虫的组合物和方法。 特别地，提供了对至少玉米根虫具有毒性的新型工程化杂合杀虫蛋白（eHIP）。 通过融合至少两种不同的苏云金芽孢杆菌（Bt）Cry蛋白或修饰的Cry蛋白的完全或部分可变区和保守嵌段的独特组合，设计具有抗玉米根虫活性的eHIP。 还提供了编码新型eHIP的核酸分子。 还公开了制备eHIP的方法和使用编码本发明的eHIP的eHIP和核酸的方法，例如在转基因植物中赋予防御昆虫损伤的方法。

公开(公告)号：US20100017914A1

公开(公告)日：2010-01-21

申请号：US12529246

申请日：2008-03-26

申请人： Hope Hart ,  Jeng S. Chen ,  Cheryl Stacy ,  Frederick Walters

发明人： Hope Hart ,  Jeng S. Chen ,  Cheryl Stacy ,  Frederick Walters

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07K19/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K1/00

CPC分类号： C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins (eHIPs) having toxicity to at least corn rootworm are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different Bacillus thuringiensis (Bt) Cry proteins or a modified Cry proteins an eHIP having activity against corn rootworm is designed. Nucleic acid molecules encoding the novel eHIPs are also provided. Methods of making the eHIPs and methods of using the eHIPs and nucleic acids encoding the eHIPs of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.

摘要翻译： 公开了用于控制植物害虫的组合物和方法。 特别地，提供了对至少玉米根虫具有毒性的新型工程化杂合杀虫蛋白（eHIP）。 通过融合至少两种不同的苏云金芽孢杆菌（Bt）Cry蛋白或修饰的Cry蛋白的完全或部分可变区和保守嵌段的独特组合，设计具有抗玉米根虫活性的eHIP。 还提供了编码新型eHIP的核酸分子。 还公开了制备eHIP的方法和使用编码本发明的eHIP的eHIP和核酸的方法，例如在转基因植物中赋予防御昆虫损伤的方法。