公开(公告)号：US09809595B2

公开(公告)日：2017-11-07

申请号：US14770708

申请日：2014-02-27

Applicant: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL CORPORATION

Inventor： Fadel Alexis Samatey ,  Vladimir A Meshcheryakov ,  Hideyuki Matsunami ,  Akio Kitao

IPC: C07D473/08 ,  A61K31/505 ,  A61K31/522 ,  C12Q1/02 ,  C12Q1/18 ,  C07D239/49

CPC classification number: C07D473/08 ,  A61K31/505 ,  A61K31/522 ,  C07D239/49 ,  C12Q1/025 ,  C12Q1/18 ,  G01N2333/255 ,  G01N2500/04 ,  Y02A50/406 ,  Y02A50/473 ,  Y02A50/475

Abstract: The present invention relates to a method for screening a compound that inhibits secretion of toxins into host-cell cytoplasm by virulent bacteria using a needle type III secretion system. The compound of the invention is selected by screening for a compound which interacts with a loop region of the cytoplasmic domain of the membrane protein FlhB from Salmonella typhimurium or a paralog thereof. Compositions including the compound of the invention, use of the compound, and methods of treating disorders caused by virulent bacteria are also provided.