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公开(公告)号：US20240327774A1
公开(公告)日：2024-10-03
申请号：US18128441
申请日：2023-03-30
发明人： Wen-Chie HUANG , Chein-Hsun WANG , Y. H. CHEN , Chin-Hsiang CHANG , Jenping KU , Yu-Chih LIANG
CPC分类号： C12M35/02 , B01L3/502761 , C12M23/16 , B01L2200/0663 , B01L2300/0681 , B01L2400/0439 , B01L2400/06
摘要： A microfluidic electroporation device for exogenous molecules transfection is disclosed. The microfluidic electroporation device includes an electroporation chamber assembly, an ultrasound vibrator, and a controller. The electroporation chamber assembly includes an input chamber for exogenous molecules, a MEMS filter, an activation chamber and a MEMS plate. The MEMS plate holds cells within individual cavity for electroporation. Both the MEMS filter and the MEMS plate are made of semiconductor process by wet etching and/or ICP dry etching with V-shaped cavities. The top surfaces of the MEMS filter and the MEMS plate are coated with metal layer for applying electric field during the electroporation process. The electroporation chamber assembly is attached to an ultrasound vibrator which is operated intermittently to allow cells to be fixed in the cavity of the MEMS plate during electroporation process and popped out for collection after electroporation process.
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公开(公告)号：US20240326046A1
公开(公告)日：2024-10-03
申请号：US18127011
申请日：2023-03-28
发明人： Wen-Chie HUANG , Chein-Hsun WANG , Y. H. CHEN , Chin-Hsiang CHANG , Jenping KU , Yu-Chih LIANG
CPC分类号： B01L3/502753 , B01D35/06 , B01D35/1573 , B01D35/20 , B01D39/2068 , B01D63/088 , B01D71/0213 , B01D2313/18 , B01L2200/0652 , B01L2300/0681 , B01L2400/0439
摘要： A microfluidic purification device for exosomes purification is disclosed that has high speed, high viability and efficiency plus re-cycling of cells for regrowth of exosomes. The microfluidic purification device contains coarse filtering, and/or medium filtering plus fine filter with the medium/fine filter made of MEMS semiconductor process. For high-speed operation, an ultrasound vibrator attached to input chamber/filter/output chamber assembly is also used that the vibration amplitude, duty cycle and duration can be controlled through controller. The MEMS filter is V-shaped and/or funnel shape made of silicon wafer by semiconductor process. For funnel shape MEMS filter, the exit hole size is between 0.2 μm to 1 μm suitable for exosomes filtering with high speed and high viability.
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