公开(公告)号：US10287541B2

公开(公告)日：2019-05-14

申请号：US14897298

申请日：2014-05-09

Applicant: OSAKA UNIVERSITY

Inventor： Kiyotoshi Sekiguchi ,  Ko Tsutsui

IPC: C12M1/00 ,  C12M1/12 ,  C12N5/00 ,  C12N9/10 ,  C07K14/78 ,  C07K14/79 ,  C07K14/435 ,  C07K14/765

Abstract: Provided is a cell culture vessel characterized in that a surface to be in contact with cells is coated with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in a dry state, the laminin fragment being derived from at least one kind selected from laminin α5β1γ1 and laminin α5β2γ1, the cell culture vessel being any of the following: (1) a cell culture vessel of which a surface to be in contact with cells is coated only with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in a dry state; (2) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in combination with a laminin fragment having no integrin α6β1 binding activity in a dry state; and (3) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in combination with a protein that is neither a laminin nor a laminin fragment, in a dry state.

公开(公告)号：US10428311B2

公开(公告)日：2019-10-01

申请号：US15325743

申请日：2015-07-15

Applicant: OSAKA UNIVERSITY

Inventor： Kiyotoshi Sekiguchi ,  Ko Tsutsui

IPC: C12N5/074 ,  C12N15/00 ,  C12N15/09

Abstract: The present invention provides a novel technique in a cell culture method using a cell culture vessel coated with a laminin fragment, which novel technique achieves cell culture as in the case of using a recommended coating concentration even when the coating concentration is lower than the recommended coating concentration. The present invention relates to a method for enhancing an activity for mammalian cultured cells of a laminin fragment or a variant thereof each having integrin binding activity.