公开(公告)号：US10942127B2

公开(公告)日：2021-03-09

申请号：US15030183

申请日：2014-10-20

Applicant: OSAKA UNIVERSITY

Inventor： Hiroyuki Yoshikawa ,  Eiichi Tamiya ,  Shuhei Imura

IPC: C12Q1/26 ,  G01N21/17 ,  G01N21/82 ,  G01N33/58 ,  G01N33/535 ,  G01N21/78 ,  G01N21/77

Abstract: The present invention provide a method for detecting a test substance utilizing a color development caused by an enzyme reaction, or a color development caused by a specific interaction such as an antigen-antibody reaction and an enzyme reaction, the test substance being detected rapidly, sensitively and quantitatively without using a spectroscopic measurement device. The method for measuring a concentration of a test substance by the present invention comprises the steps of: generating a peroxide from the test substance; obtaining a polymerized substance by bringing an oxidoreductase for producing a polymerized substance and a substrate of the oxidoreductase for producing a polymerized substance into contact with the peroxide; and irradiating the polymerized substance with light to record a temporal variation information of an intensity of scattered light generated from an irradiation point.