MASS SPECTROMETER OUTPUT ANALYSIS TOOL FOR IDENTIFICATION OF PROTEINS
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    发明申请
    MASS SPECTROMETER OUTPUT ANALYSIS TOOL FOR IDENTIFICATION OF PROTEINS 审中-公开
    质谱仪输出分析用于鉴定蛋白质的工具

    公开(公告)号:US20100280759A1

    公开(公告)日:2010-11-04

    申请号:US12473005

    申请日:2009-05-27

    IPC分类号: G06F19/00 G01N33/68

    CPC分类号: G16B45/00 G16B30/00 G16B50/00

    摘要: Methods and apparatus for consolidation and visualization of a dataset output from a mass spectrometer. The number of proteins identified in a mass spectrometry experiment may be systematically reduced by identifying and excluding proteins which may not be necessary to explain the observed peptides in a sample. Peptide counts from excluded proteins may be redistributed to non-excluded proteins to preserve total observed peptide counts. The consolidated dataset may be represented as a data structure having a plurality of cells arranged into rows and columns. Visual parameter values may be assigned to various cells in the data structure to emphasize particular features of the data. Data from multiple samples may be arranged in sets of columns in the data structure, and difference scores may be calculated to determine peptide expression differences between the multiples samples. The difference scores may be displayed using textual and/or graphical representations to facilitate data analysis.

    摘要翻译: 用于整合和显示质谱仪输出的数据集的方法和装置。 通过鉴定和排除可能不需要解释样品中观察到的肽的蛋白质,可以系统地减少在质谱实验中鉴定的蛋白质的数量。 来自排除蛋白的肽计数可以重新分配到未排除的蛋白质以保留总观察到的肽计数。 统一数据集可以表示为具有排列成行和列的多个单元的数据结构。 可视参数值可以分配给数据结构中的各个单元,以强调数据的特定特征。 来自多个样本的数据可以以数据结构中的列的形式排列,并且可以计算差分以确定多个样本之间的肽表达差异。 可以使用文本和/或图形表示来显示差异分数以促进数据分析。