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公开(公告)号:US20230227903A1
公开(公告)日:2023-07-20
申请号:US18010826
申请日:2021-06-18
发明人: Rebecca Victoria Bowen , Clive Gavin Brown , Mark John Bruce , Daniel Ryan Garalde , James Edward Graham , Andrew John Heron , Etienne Raimondeau , James White , Christopher Peter Youd
IPC分类号: C12Q1/6869
CPC分类号: C12Q1/6869
摘要: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
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公开(公告)号:US20230024319A1
公开(公告)日:2023-01-26
申请号:US17781469
申请日:2020-12-01
IPC分类号: G01N33/487 , G01N33/68 , C12N15/11
摘要: Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.
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公开(公告)号:US20240200128A1
公开(公告)日:2024-06-20
申请号:US18352701
申请日:2023-07-14
发明人: Andrew John Heron , James Edward Graham , Richard Alexander Gutierrez , Rebecca Victoria Bowen , James White , Clive Gavin Brown , Daniel George Fordham
IPC分类号: C12Q1/6869 , C12Q1/6876 , G01N33/487
CPC分类号: C12Q1/6869 , C12Q1/6876 , G01N33/48721
摘要: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore: (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.
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公开(公告)号:US20240044865A1
公开(公告)日:2024-02-08
申请号:US18357504
申请日:2023-07-24
IPC分类号: G01N33/487 , C12N15/11 , G01N33/68
CPC分类号: G01N33/48721 , C12N15/11 , G01N33/6818 , C12N2310/3517 , G01N2458/00
摘要: Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.
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5.
公开(公告)号:US20220403368A1
公开(公告)日:2022-12-22
申请号:US17763716
申请日:2020-09-25
IPC分类号: C12N15/10
摘要: A method of preparing a nucleic acid construct for single molecule characterisation, comprising contacting a target polynucleotide with: a polynucleotide-guided effector protein, a guide polynucleotide; a transposase; and a transposable element comprising a modified polynucleotide, wherein the polynucleotide-guided effector protein directs said transposase to a region of interest within the target polynucleotide and the transposase inserts the transposable element into the polynucleotide, thereby producing a nucleic acid construct for single molecule characterisation.
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公开(公告)号:US20230295712A1
公开(公告)日:2023-09-21
申请号:US18010825
申请日:2021-06-18
发明人: Rebecca Victoria Bowen , Clive Gavin Brown , Mark John Bruce , Daniel Ryan Garalde , James Edward Graham , Andrew John Heron , Etienne Raimondeau , James White , Christopher Peter Youd
IPC分类号: C12Q1/6869
CPC分类号: C12Q1/6869
摘要: The invention provides a method of selectively characterising polynucleotides of a desired property, such as length, using a nanopore, based on the translocation of the polynucleotide through or across the nanopore. Kits and systems for use in such methods are also provided. The methods of the invention are particularly suitable for sequencing polynucleotides such as DNA.
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公开(公告)号:US11739379B2
公开(公告)日:2023-08-29
申请号:US17361821
申请日:2021-06-29
发明人: Andrew John Heron , James Edward Graham , Richard Alexander Gutierrez , Rebecca Victoria Bowen , James White , Clive Gavin Brown , Daniel George Fordham
IPC分类号: C12Q1/68 , C12Q1/6869 , C12Q1/6876 , G01N33/487
CPC分类号: C12Q1/6869 , C12Q1/6876 , G01N33/48721
摘要: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.
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公开(公告)号:US20230227902A1
公开(公告)日:2023-07-20
申请号:US18010824
申请日:2021-06-18
发明人: James Edward Graham , Christopher Peter Youd , Clive Gavin Brown , Marion Louise Crawford , Andrew John Heron , James White
IPC分类号: C12Q1/6869
CPC分类号: C12Q1/6869
摘要: Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.
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公开(公告)号:US20240076729A9
公开(公告)日:2024-03-07
申请号:US18010826
申请日:2021-06-18
发明人: Rebecca Victoria Bowen , Clive Gavin Brown , Mark John Bruce , Daniel Ryan Garalde , James Edward Graham , Andrew John Heron , Etienne Raimondeau , James White , Christopher Peter Youd
IPC分类号: C12Q1/6869
CPC分类号: C12Q1/6869
摘要: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
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