公开(公告)号：US12216110B2

公开(公告)日：2025-02-04

申请号：US15487329

申请日：2017-04-13

Applicant: Oxford Nanopore Technologies PLC

Inventor： Stuart William Reid ,  Gavin Harper ,  Clive Gavin Brown ,  James Anthony Clarke ,  Andrew John Heron

IPC: G01N33/483 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487 ,  G06F17/18 ,  G06N7/01 ,  G16B30/00 ,  G16B30/10 ,  B82Y15/00

Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.

公开(公告)号：US12215384B2

公开(公告)日：2025-02-04

申请号：US16304077

申请日：2017-05-25

Applicant: Oxford Nanopore Technologies PLC

Inventor： Richard Alexander Gutierrez ,  Andrew John Heron ,  James White

IPC: C12Q1/6869

Abstract: The invention relates to a new method of characterising a target polynucleotide using a pore. The method involves controlling the formation of secondary structure by the target polynucleotide after the polynucleotide has moved through the pore.

公开(公告)号：US20240417704A1

公开(公告)日：2024-12-19

申请号：US18762324

申请日：2024-07-02

Applicant: Oxford Nanopore Technologies PLC

Inventor： Andrew John Heron ,  James Anthony Clarke ,  Ruth Moysey ,  Elizabeth Jayne Wallace ,  Mark John Bruce ,  Lakmal Nishantha Jayasinghe ,  Domenico Caprotti ,  Szabolcs Soeroes ,  Luke McNeill ,  David Antoni Alves ,  Rebecca Victoria Bowen ,  John Milton

IPC: C12N9/14 ,  C12N9/90 ,  C12Q1/6827

Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

公开(公告)号：US20240253005A1

公开(公告)日：2024-08-01

申请号：US18614432

申请日：2024-03-22

Applicant: Oxford Nanopore Technologies PLC

Inventor： Jason Robert Hyde ,  Pedro Miguel Ortiz Bahamon ,  Clive Gavin Brown ,  Andrew John Heron ,  Paul Raymond Mackett

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487 ,  G01N33/573

CPC classification number: B01J19/0046 ,  B01L3/5088 ,  C12Q1/6869 ,  G01N33/48721 ,  G01N33/573 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00734 ,  B01J2219/00736 ,  B01L2200/0642 ,  B01L2400/086 ,  G01N2333/974

Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

公开(公告)号：US20240240245A1

公开(公告)日：2024-07-18

申请号：US17612606

申请日：2020-05-22

Applicant: Oxford Nanopore Technologies plc

Inventor： Andrew John Heron ,  Mark John Bruce ,  Rebecca Victoria Bowen ,  Luke Alexander McNeill ,  Simon Rafael Villarreal ,  Samuel John Martin ,  Rebecca Anne Stafford-Allen

IPC: C12Q1/6869 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein is a method of loading a motor protein onto a polynucleotide adapter. Also provided are polynucleotide adapters and kits comprising such adapters. The adapters find use in characterising analytes such as polynucleotides in methods in which the polynucleotide moves in respect of a nanopore.

公开(公告)号：US20240076729A9

公开(公告)日：2024-03-07

申请号：US18010826

申请日：2021-06-18

Applicant: Oxford Nanopore Technologies PLC

Inventor： Rebecca Victoria Bowen ,  Clive Gavin Brown ,  Mark John Bruce ,  Daniel Ryan Garalde ,  James Edward Graham ,  Andrew John Heron ,  Etienne Raimondeau ,  James White ,  Christopher Peter Youd

IPC: C12Q1/6869

CPC classification number: C12Q1/6869

Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

公开(公告)号：US20230212535A1

公开(公告)日：2023-07-06

申请号：US18047019

申请日：2022-10-17

Applicant: Oxford Nanopore Technologies PLC

Inventor： Mark Bruce ,  Andrew John Heron ,  Ruth Moysey ,  Szabolcs Soeroes ,  Elizabeth Jayne Wallace ,  James White

IPC: C12N9/14 ,  C12N9/90 ,  C12Q1/6869

CPC classification number: C12N9/14 ,  C12N9/90 ,  C12Q1/6869 ,  C12Q2565/631 ,  C12Y306/04012

Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

公开(公告)号：US20230158503A1

公开(公告)日：2023-05-25

申请号：US17989814

申请日：2022-11-18

Applicant: Oxford Nanopore Technologies PLC ,  Sharp Life Science EU (Ltd)

Inventor： Matthew Holden ,  James White ,  Andrew John Heron ,  James Anthony Clarke ,  Jason Robert Hyde ,  Benjamin James Hadwen ,  Sally Anderson

IPC: B01L3/00 ,  C12Q1/6869 ,  G01N33/487

CPC classification number: B01L3/502792 ,  C12Q1/6869 ,  G01N33/48721 ,  B01L2300/165 ,  B01L2300/0645 ,  B01L2400/0427

Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

公开(公告)号：US11525125B2

公开(公告)日：2022-12-13

申请号：US16893332

申请日：2020-06-04

Applicant: Oxford Nanopore Technologies PLC

Inventor： Mark John Bruce ,  Andrew John Heron ,  Ruth Moysey ,  Szabolcs Soeroes ,  Elizabeth Jayne Wallace ,  James White

IPC: C12N9/14 ,  C12N9/90 ,  C12Q1/6869

Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

公开(公告)号：US20240124915A9

公开(公告)日：2024-04-18

申请号：US18170596

申请日：2023-02-17

Applicant: Oxford Nanopore Technologies PLC

Inventor： Clive Gavin Brown ,  Daniel Ryan Garalde ,  Andrew John Heron ,  Daniel John Turner ,  James White

IPC: C12Q1/6804 ,  C12N9/52 ,  C12N15/10 ,  C12Q1/6869 ,  G01N33/487 ,  G01N33/68

CPC classification number: C12Q1/6804 ,  C12N9/52 ,  C12N15/1013 ,  C12Q1/6869 ,  G01N33/48721 ,  G01N33/48728 ,  G01N33/6872 ,  B03C2201/18 ,  C12Q2523/303 ,  C12Q2525/197 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/631

Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.