公开(公告)号：US10738334B2

公开(公告)日：2020-08-11

申请号：US15762345

申请日：2016-09-22

申请人： PFIZER INC.

发明人： Gregory Walter Hiller ,  Jeffrey Joseph Mitchell ,  Bhanu Chandra Mulukutla ,  Pamela Mary Pegman

IPC分类号： C12N15/11 ,  C12P7/62 ,  C12P7/42 ,  C12P7/52 ,  C07K16/00 ,  C12P7/56 ,  C12P7/40 ,  C12P17/18

摘要： The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

公开(公告)号：US11718864B2

公开(公告)日：2023-08-08

申请号：US16917099

申请日：2020-06-30

申请人： PFIZER INC.

发明人： Gregory Walter Hiller ,  Jeffrey Joseph Mitchell ,  Bhanu Chandra Mulukutla ,  Pamela Mary Pegman

IPC分类号： C12N15/62 ,  C12P7/62 ,  C12P7/42 ,  C12P7/52 ,  C07K16/00 ,  C12P7/56 ,  C12P7/40 ,  C12P17/18

CPC分类号： C12P7/62 ,  C07K16/00 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P7/56 ,  C12P17/182 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2511/00

摘要： The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.