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    CLONING AND USE OF THE MS9 GENE FROM MAIZE
    1.
    发明申请
    CLONING AND USE OF THE MS9 GENE FROM MAIZE 审中-公开
    从MAIZE克隆和使用MS9基因

    公开(公告)号：US20160024520A1

    公开(公告)日：2016-01-28

    申请号：US14773465

    申请日：2014-03-14

    申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DUPONT DE NEMOURS & COMPANY

    发明人： Marc C. Albertsen ,  TIM FOX ,  APRIL LEONARD ,  BAILIN LI ,  BRIAN LOVELAND ,  MARY TRIMNELL

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8289 ,  A01H1/02 ,  A01H5/10 ,  C07K14/415 ,  C12N15/8218 ,  C12N15/8231 ,  C12N15/8265

    摘要： Nucleotide sequences, amino acid sequences, and an associated promoter sequence are useful in methods of controlling male fertility in plants. Recombinant expression cassettes, vectors, plant cells, and plants comprise a disclosed nucleotide sequence and may encode a disclosed amino acid sequence. The recombinant expression cassettes are useful in controlling fertility, especially male fertility of annual crops. The promoter is useful in driving expression of an operably-linked heterologous polynucleotide. Constructs comprising homology to the promoter sequence are useful for downregulation of a polynucleotide associated with the promoter.

    摘要翻译： 核苷酸序列，氨基酸序列和相关启动子序列可用于控制植物中雄性生殖力的方法。 重组表达盒，载体，植物细胞和植物包含公开的核苷酸序列，并且可以编码公开的氨基酸序列。 重组表达盒可用于控制一年生作物的生育力，特别是雄性生育力。 启动子可用于驱动可操作连接的异源多核苷酸的表达。 与启动子序列同源的构建体可用于与启动子相关的多核苷酸的下调。

    COMPOSITIONS AND METHODS OF MODIFYING A PLANT GENOME TO PRODUCE A MS9, MS22, MS26, or MS45 MALE-STERILE PLANT
    4.
    发明申请
    COMPOSITIONS AND METHODS OF MODIFYING A PLANT GENOME TO PRODUCE A MS9, MS22, MS26, or MS45 MALE-STERILE PLANT 有权

    公开(公告)号：US20220056439A1

    公开(公告)日：2022-02-24

    申请号：US17454510

    申请日：2021-11-11

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： ANDREW MARK CIGAN ,  TIM FOX ,  MANJIT SINGH

    IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/82 ,  C07K14/415

    摘要： Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.

    COMPOSITIONS AND METHODS OF MODIFYING A PLANT GENOME TO PRODUCE A MS9, MS22, MS26, OR MS45 MALE-STERILE PLANT
    7.
    发明申请
    COMPOSITIONS AND METHODS OF MODIFYING A PLANT GENOME TO PRODUCE A MS9, MS22, MS26, OR MS45 MALE-STERILE PLANT 审中-公开

    公开(公告)号：US20190177722A1

    公开(公告)日：2019-06-13

    申请号：US16214897

    申请日：2018-12-10

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： ANDREW MARK CIGAN ,  TIM FOX ,  MANJIT SINGH

    IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/82

    摘要： Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.

    FERTILITY RESTORATION IN PLANTS
    8.
    发明申请
    FERTILITY RESTORATION IN PLANTS 有权

    公开(公告)号：US20220056472A1

    公开(公告)日：2022-02-24

    申请号：US17275111

    申请日：2019-09-13

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： MARC C ALBERTSEN ,  TIM FOX ,  MANJIT SINGH ,  MARK E WILLIAMS

    IPC分类号： C12N15/82

    摘要： Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.