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    Compositions Having Dicamba Decarboxylase Activity and Methods of Use
    2.
    发明申请
    Compositions Having Dicamba Decarboxylase Activity and Methods of Use 审中-公开
    具有麦草畏脱羧酶活性和使用方法的组合物

    公开(公告)号：US20140287419A1

    公开(公告)日：2014-09-25

    申请号：US14214571

    申请日：2014-03-14

    申请人： Arzeda Corp. ,  Pioneer Hi-Bred International, Inc.

    发明人： Eric Althoff ,  Yih-En Andrew Ban ,  Linda A. Castle ,  Daniela Grabs ,  Jian Lu ,  Phillip A. Patten ,  Yumin Tao ,  Alexandre Zanghellini

    IPC分类号： C12N9/88 ,  C12Q1/68 ,  G01N33/573 ,  C12N15/63 ,  C12P7/00

    CPC分类号： C12N15/8274 ,  A01G22/00 ,  A01N37/10 ,  A01N37/40 ,  A01N57/20 ,  C12N9/88 ,  C12N15/63 ,  C12P7/00 ,  C12Q1/686 ,  G01N33/573

    摘要： Compositions and methods comprising polynucleotides and polypeptides having dicamba decarboxylase activity are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the dicamba decarboxylase sequences. Various methods of employing the dicamba decarboxylase sequences are provided. Such methods include, for example, methods for decarboxylating an auxin-analog, method for producing an auxin-analog tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional dicamba decarboxylase variants.

    摘要翻译： 提供了包含具有麦草酸脱羧酶活性的多核苷酸和多肽的组合物和方法。 还提供了具有麦草畏脱羧酶序列的核酸构建体，宿主细胞，植物，植物细胞，外植体，种子和谷粒。 提供了使用麦草畏脱羧酶序列的各种方法。 这样的方法包括例如生长素类似物脱羧的方法，用于生产生长素 - 类似物耐受性植物，植物细胞，外植体或种子的方法和在含有使用所述植物和/或种子的作物的田地中控制杂草的方法 这里。 还提供了用于鉴定另外的麦草酸脱羧酶变体的方法。

    Methods and Compositions for Targeting Sequences of Interest to the Chloroplast
    3.
    发明申请
    Methods and Compositions for Targeting Sequences of Interest to the Chloroplast 审中-公开
    靶向叶绿体序列的方法和组成

    公开(公告)号：US20160017351A1

    公开(公告)日：2016-01-21

    申请号：US14835242

    申请日：2015-08-25

    申请人： Pioneer Hi-Bred International, Inc.

    发明人： Henrik Albert ,  Linda A. Castle ,  Matthew Heckert ,  Jian Lu ,  Daniel L. Siehl ,  Yumin Tao

    IPC分类号： C12N15/82 ,  C12N9/02

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO: 1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

    摘要翻译： 提供了包含编码与目标异源多核苷酸有效连接的叶绿体转运肽的核苷酸序列的嵌合多核苷酸，其中叶绿体转运肽包含具有如SEQ ID NO：1所示的叶绿体转运肽序列的氨基酸序列或生物学上 活性变体或其片段，或其中叶绿体转运肽包含SEQ ID NO：58所示的序列或其活性变体或片段。 还提供了编码其的嵌合多肽，以及细胞，植物细胞，植物和种子，其包含嵌合多核苷酸。 组合物还包括编码与SEQ ID NO：57和60所示相同的HPPD多肽和多核苷酸或其活性变体和片段。 这样的序列包含如SEQ ID NO：58所示的叶绿体转运肽或其活性变体或片段。 进一步提供包含这些序列的细胞，植物细胞，植物和种子。 还提供了使用各种序列的方法。

    Plant EPSP synthases and methods of use
    5.
    发明授权
    Plant EPSP synthases and methods of use 有权

    公开(公告)号：US11549123B2

    公开(公告)日：2023-01-10

    申请号：US16816349

    申请日：2020-03-12

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Yuxia Dong ,  Michael Lassner ,  Jian Lu ,  Emily Ng ,  Phillip A Patten ,  Daniel Siehl

    IPC分类号： C12N15/82 ,  C12N9/10

    摘要： Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

    Plant EPSP synthases and methods of use
    6.
    发明授权
    Plant EPSP synthases and methods of use 有权

    公开(公告)号：US10655141B2

    公开(公告)日：2020-05-19

    申请号：US15764388

    申请日：2016-09-29

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Yuxia Dong ,  Michael Lassner ,  Jian Lu ,  Emily Ng ,  Phillip A. Patten ,  Daniel Siehl

    IPC分类号： C12N15/82 ,  C12N9/10

    摘要： Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

    CHIMERIC PROMOTERS AND METHODS OF USE
    8.
    发明申请
    CHIMERIC PROMOTERS AND METHODS OF USE 审中-公开
    CHIMERIC PROMOTERS和使用方法

    公开(公告)号：US20150275190A1

    公开(公告)日：2015-10-01

    申请号：US14630309

    申请日：2015-02-24

    申请人： Pioneer Hi-Bred International, Inc.

    发明人： Henrik Albert ,  Linda A. Castle ,  Jian Lu ,  Yumin Tao

    IPC分类号： C12N9/02 ,  C12N15/82

    CPC分类号： C12N15/8221 ,  C12N9/0069 ,  C12N15/82 ,  C12N15/8274 ,  C12Y113/11027

    摘要： Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

    摘要翻译： 提供了包含来自4-羟基苯基丙酮酸双加氧酶（HPPD）基因及其活性变体和片段的启动子的组合物和方法，以及采用HPPD启动子调节区的嵌合启动子。 还提供了包含本文公开的各种启动子的表达盒和植物，其与感兴趣的多核苷酸可操作地连接。 进一步提供了使用本文所述的各种启动子来调节感兴趣的多核苷酸的表达的方法。