公开(公告)号：US10590372B2

公开(公告)日：2020-03-17

申请号：US15560561

申请日：2016-03-03

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Jae Sung Park ,  Hyun Woo Shin

IPC: C12M1/26 ,  C12Q1/68 ,  C12Q1/24 ,  C12N13/00 ,  G01N33/53 ,  C12M1/42 ,  C12N5/00 ,  B01D17/02 ,  B01D11/04 ,  C12Q1/6806 ,  G01N33/535 ,  C12Q1/686 ,  G01N33/68

Abstract: This disclosure relates to a multi-stage method and apparatus for purifying extracellular vesicles using an aqueous two-phase system, in which extracellular vesicles mixed and contaminated with proteins can be isolated and purified in a large amount at high purity within a short time through a multi-stage purification process using an aqueous two-phase system, thereby removing 95% or more of proteins and obtaining high-purity extracellular vesicles, resulting in very high processing efficiency compared to conventional techniques. In particular, the method of the disclosure does not require an expensive device or material such as an ultracentrifuge or an antibody, and can be performed at low cost and is thus economical and highly competitive. Furthermore, the extracellular vesicles thus isolated and purified can be employed in analysis methods such as RT-PCR or western blot, and can be utilized for research fields and disease diagnosis using the same.

公开(公告)号：US12194415B2

公开(公告)日：2025-01-14

申请号：US17316864

申请日：2021-05-11

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION ,  EXOSOMEPLUS INC.

Inventor： Jae Sung Park ,  Hyun Woo Shin ,  Hwa Pyeong Jeong ,  Si Woo Cho

IPC: B01D43/00 ,  B01D61/02 ,  B01D61/10 ,  B01D61/12 ,  B01D69/02 ,  C02F1/44

Abstract: Proposed are an aqueous two-phase system nanofilter capable of effectively separating particles having different sizes within a short time, and a separation method using same. The aqueous two-phase system nanofilter can separate mixed nanoparticles having a size difference of approximately 10 nm, through the designing of a composition constituting each composition. The separated nanoparticles can be applied across industries such as electronics, photoelectronics and magnetic fields, biomedical, medical and cosmetic fields, energy, catalysts, and structures.

公开(公告)号：US11016009B2

公开(公告)日：2021-05-25

申请号：US15562549

申请日：2016-03-03

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION ,  THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Jae Sung Park ,  Joseph M Labuz ,  Shuichi Takayama ,  Hyun Woo Shin ,  Chung Min Han

IPC: G01N1/40 ,  C12M1/26 ,  C12Q1/24 ,  C12Q1/68 ,  G01N33/53 ,  A61K35/12 ,  B01D17/02 ,  C12Q1/6806

Abstract: Disclosed is a method of isolating extracellular vesicles using an aqueous two-phase system (ATPS), including (a) preparing an ATPS by mixing a first material and a second material, which are immiscible with each other, with a body fluid or an aqueous solution containing extracellular vesicles and (b) isolating extracellular vesicles concentrated in the second material of the ATPS. This method can exhibit very high isolation efficiency, a simple isolation manner, and a very short isolation time. The isolation of extracellular vesicles using the ATPS requires no ultracentrifuge and achieves almost 100% isolation efficiency within a short time of about 10˜20 min, and thus the method of the invention is practical, is economical due to low costs thereof, can increase the purity of extracellular vesicles contaminated with protein, enables the diagnosis of disease using the isolated extracellular vesicles, and can be applied to various fields using extracellular vesicles.