公开(公告)号：US10590372B2

公开(公告)日：2020-03-17

申请号：US15560561

申请日：2016-03-03

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Jae Sung Park ,  Hyun Woo Shin

IPC: C12M1/26 ,  C12Q1/68 ,  C12Q1/24 ,  C12N13/00 ,  G01N33/53 ,  C12M1/42 ,  C12N5/00 ,  B01D17/02 ,  B01D11/04 ,  C12Q1/6806 ,  G01N33/535 ,  C12Q1/686 ,  G01N33/68

Abstract: This disclosure relates to a multi-stage method and apparatus for purifying extracellular vesicles using an aqueous two-phase system, in which extracellular vesicles mixed and contaminated with proteins can be isolated and purified in a large amount at high purity within a short time through a multi-stage purification process using an aqueous two-phase system, thereby removing 95% or more of proteins and obtaining high-purity extracellular vesicles, resulting in very high processing efficiency compared to conventional techniques. In particular, the method of the disclosure does not require an expensive device or material such as an ultracentrifuge or an antibody, and can be performed at low cost and is thus economical and highly competitive. Furthermore, the extracellular vesicles thus isolated and purified can be employed in analysis methods such as RT-PCR or western blot, and can be utilized for research fields and disease diagnosis using the same.

公开(公告)号：US09081012B2

公开(公告)日：2015-07-14

申请号：US13826485

申请日：2013-03-14

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Jae Sung Park ,  Jun Ho Kim ,  Ryan Thomas Davies

IPC: C12Q1/68 ,  C12P19/34 ,  G01N33/574 ,  B01L3/00 ,  G01N1/40

CPC classification number: G01N33/574 ,  B01L3/502753 ,  C12P19/34 ,  C12Q1/68 ,  G01N2001/4088

Abstract: The present invention relates to an apparatus for the isolation of extracellular vesicles from human fluid, and more particularly, to an apparatus comprising a channel formed on a microchip and a porous polymer monolith filter connected to the channel. The apparatus can be used to the diagnosis of disease including cancer from vesicular nucleic acid in a non-invasive manner. Capable of isolating and purifying a large quantity of vesicles from a small amount of a body fluid sample within a short time, the apparatus is expected to be advantageously and widely applied in the medical research and clinical diagnosis of disease including cancer.

Abstract translation: 本发明涉及一种用于从人体流体分离细胞外囊泡的装置，更具体地，涉及一种包括形成在微芯片上的通道和连接到通道的多孔聚合物整体滤清器的装置。 该装置可用于以非侵入性方式从囊泡核酸诊断包括癌症的疾病。 能够在短时间内从少量的体液样品中分离并净化大量的囊泡，预期该装置将有利地广泛地应用于包括癌症在内的疾病的医学研究和临床诊断中。

公开(公告)号：US12194415B2

公开(公告)日：2025-01-14

申请号：US17316864

申请日：2021-05-11

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION ,  EXOSOMEPLUS INC.

Inventor： Jae Sung Park ,  Hyun Woo Shin ,  Hwa Pyeong Jeong ,  Si Woo Cho

IPC: B01D43/00 ,  B01D61/02 ,  B01D61/10 ,  B01D61/12 ,  B01D69/02 ,  C02F1/44

Abstract: Proposed are an aqueous two-phase system nanofilter capable of effectively separating particles having different sizes within a short time, and a separation method using same. The aqueous two-phase system nanofilter can separate mixed nanoparticles having a size difference of approximately 10 nm, through the designing of a composition constituting each composition. The separated nanoparticles can be applied across industries such as electronics, photoelectronics and magnetic fields, biomedical, medical and cosmetic fields, energy, catalysts, and structures.

公开(公告)号：US11016009B2

公开(公告)日：2021-05-25

申请号：US15562549

申请日：2016-03-03

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION ,  THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Jae Sung Park ,  Joseph M Labuz ,  Shuichi Takayama ,  Hyun Woo Shin ,  Chung Min Han

IPC: G01N1/40 ,  C12M1/26 ,  C12Q1/24 ,  C12Q1/68 ,  G01N33/53 ,  A61K35/12 ,  B01D17/02 ,  C12Q1/6806

Abstract: Disclosed is a method of isolating extracellular vesicles using an aqueous two-phase system (ATPS), including (a) preparing an ATPS by mixing a first material and a second material, which are immiscible with each other, with a body fluid or an aqueous solution containing extracellular vesicles and (b) isolating extracellular vesicles concentrated in the second material of the ATPS. This method can exhibit very high isolation efficiency, a simple isolation manner, and a very short isolation time. The isolation of extracellular vesicles using the ATPS requires no ultracentrifuge and achieves almost 100% isolation efficiency within a short time of about 10˜20 min, and thus the method of the invention is practical, is economical due to low costs thereof, can increase the purity of extracellular vesicles contaminated with protein, enables the diagnosis of disease using the isolated extracellular vesicles, and can be applied to various fields using extracellular vesicles.