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21 条记录 (耗时 0.054 秒)

    Referenced amplification of small quantities of RNA
    4.
    发明授权
    Referenced amplification of small quantities of RNA 失效

    公开(公告)号：US06602665B2

    公开(公告)日：2003-08-05

    申请号：US09822789

    申请日：2001-03-30

    申请人： David de Graaf ,  Eric S. Lander

    发明人： David de Graaf ,  Eric S. Lander

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6809 ,  C12Q2531/113 ,  C12Q2525/143

    摘要： Methods for determining the amount of an RNA transcript in a test sample are disclosed, utilizing a reference sample comprising a known amount of a reference nucleic acid, labeled with a first strand 3′ cDNA primer and a first strand 5′ cDNA primer comprising a reference specificity determining box; and a test sample comprising an amount of test RNA containing an RNA transcript of interest, the test RNA being labeled with the first strand 3′ cDNA primer and a first strand 5′ cDNA primer comprising a test specificity determining box. The reference sample and the test sample are mixed and subjected to polymerase chain reaction amplification conditions, followed by division of the amplified, mixed sample and continued amplification of the divided sample to produce nucleic acids containing amplified reference nucleic acid or amplified cDNA of the test RNA, from which cRNA can be generated by in vitro transcription. The amount of the test RNA, or of the RNA transcript of interest, in the sample correlates with a ratio of the amount of amplified cDNA of the test RNA (or of the RNA transcript of interest), over the amount of the amplified reference nucleic acid, multiplied by the known amount of the reference nucleic acid in the reference sample.

    MULTIPLEXED GENETIC REPORTER ASSAYS AND COMPOSITIONS
    5.
    发明申请
    MULTIPLEXED GENETIC REPORTER ASSAYS AND COMPOSITIONS 审中-公开
    多功能遗传记录仪测定及组合物

    公开(公告)号：US20140200163A1

    公开(公告)日：2014-07-17

    申请号：US14115608

    申请日：2012-05-04

    申请人： Tarjei Mikkelsen ,  Andreas Gnirke ,  Alexandre Melnikov ,  Eric S. Lander ,  Li Wang ,  Xiaolan Zhang

    发明人： Tarjei Mikkelsen ,  Andreas Gnirke ,  Alexandre Melnikov ,  Eric S. Lander ,  Li Wang ,  Xiaolan Zhang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6897 ,  C12N15/1086 ,  C12N15/63

    摘要： The invention provides methods for determining the activity of a plurality of nucleic acid regulatory elements. These methods may facilitate, e.g., the systematic reverse engineering, and optimization of mammalian cis-regulatory elements at high resolution and at a large scale. The method may include integration of multiplexed DNA synthesis and sequencing technologies to generate and quantify the transcriptional regulatory activity of e.g., thousands of arbitrary DNA sequences in parallel in cell-based as says (e.g., mammalian cell based assays).

    摘要翻译： 本发明提供了确定多种核酸调节元件的活性的方法。 这些方法可以促进例如系统逆向工程，并且以高分辨率和大规模优化哺乳动物顺式调节元件。 该方法可以包括多重的DNA合成和测序技术的整合，以产生并定量例如成千上万个任意DNA序列在基于细胞的平行中的转录调节活性，如所述（例如，基于哺乳动物细胞的测定）。

    Single nucleotide polymorphisms in genes
    8.
    发明授权
    Single nucleotide polymorphisms in genes 失效
    基因单核苷酸多态性

    公开(公告)号：US06727063B1

    公开(公告)日：2004-04-27

    申请号：US09657472

    申请日：2000-09-07

    申请人： Eric S. Lander ,  Michele Cargill ,  James S. Ireland ,  Stacey Bolk ,  George Q. Daley ,  Jeanette J. McCarthy

    发明人： Eric S. Lander ,  Michele Cargill ,  James S. Ireland ,  Stacey Bolk ,  George Q. Daley ,  Jeanette J. McCarthy

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6883 ,  C07K14/78 ,  C12Q2600/156

    摘要： The invention provides nucleic acid segments of the human genome, particularly nucleic acid segments from a gene, including polymorphic sites. Allele-specific primers and probes hybridizing to regions flanking or containing these sites are also provided. The nucleic acids, primers and probes are used in applications such as phenotype correlations, forensics, paternity testing, medicine and genetic analysis. A role for the thrombospondin gene(s) in vascular disease is also disclosed. Use of single nucleotide polymorphisms in the thrombospondin gene(s) for diagnosis, prediction of clinical course and treatment response, development of therapeutics and development of cell-culture-based and animal models for research and treatment are disclosed.

    摘要翻译： 本发明提供人基因组的核酸片段，特别是来自基因的核酸片段，包括多态位点。 还提供了与侧翼或含有这些位点的区域杂交的等位基因特异性引物和探针。 核酸，引物和探针用于表型相关性，取证，亲子鉴定，药物和遗传分析等应用中。 还公开了血小板反应蛋白基因在血管疾病中的作用。 公开了在血小板反应蛋白基因中用于诊断，预测临床病程和治疗反应的单核苷酸多态性，治疗的发展以及用于研究和治疗的基于细胞培养的动物模型的开发。