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    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING
    1.
    发明申请
    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING 审中-公开

    公开(公告)号：US20170145496A1

    公开(公告)日：2017-05-25

    申请号：US15357966

    申请日：2016-11-21

    申请人： Pacific Biosciences of California, Inc.

    发明人： Lubomir SEBO ,  Gene SHEN ,  Stephen YUE ,  Honey OSUNA ,  Yuri LAPIN ,  Louis BROGLEY ,  Andrei FEDOROV

    IPC分类号： C12Q1/68 ,  C07K19/00

    摘要： Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

    MULTIMERIC PROTECTED FLUORESCENT REAGENTS
    2.
    发明申请
    MULTIMERIC PROTECTED FLUORESCENT REAGENTS 审中-公开
    多元保护荧光试剂

    公开(公告)号：US20160237279A1

    公开(公告)日：2016-08-18

    申请号：US15015602

    申请日：2016-02-04

    申请人： Pacific Biosciences of California, Inc.

    发明人： Frank ZHENG ,  Jeremiah HANES ,  Gene SHEN ,  Louis BROGLEY ,  Stephen YUE ,  Yuri LAPIN ,  John LYLE ,  Honey OSUNA ,  Andrei FEDOROV ,  Lubomir SEBO

    IPC分类号： C09B23/10

    CPC分类号： C09B69/10 ,  C09B23/06 ,  C09B23/105 ,  C09B69/00 ,  C09B69/105 ,  G01N33/533

    摘要： Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The reagents contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements. The reagents also contain coupling elements connect monomeric compounds into multimeric forms, thereby increasing brightness.

    摘要翻译： 提供了多聚体保护的荧光试剂及其合成方法。 这些试剂可用于各种基于荧光的分析方法，包括以高密度大量多重光学反应的分析，如单分子实时核酸测序反应。 试剂含有荧光染料元素，其允许以期望波长的高灵敏度检测化合物，允许化合物被目标生物分子特异性识别的结合元件以及减少荧光染料元件之间不希望的接触的保护屏蔽元件 和结合的靶生物分子，因此由荧光染料元素降低结合的靶生物分子的光损伤。 试剂还含有偶联元件将单体化合物连接成多聚体形式，从而增加亮度。

    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING
    4.
    发明申请
    LABELED NUCLEOTIDE ANALOGS, REACTION MIXTURES, AND METHODS AND SYSTEMS FOR SEQUENCING 有权

    公开(公告)号：US20210147928A1

    公开(公告)日：2021-05-20

    申请号：US17006669

    申请日：2020-08-28

    申请人： Pacific Biosciences of California, Inc.

    发明人： Lubomir SEBO ,  Gene SHEN ,  Stephen YUE ,  Honey OSUNA ,  Yuri LAPIN ,  Louis BROGLEY ,  Andrei FEDOROV

    IPC分类号： C12Q1/6869 ,  C07H19/10 ,  C07H19/207 ,  C09B69/10 ,  C07K19/00

    摘要： Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

    MULTIMERIC PROTECTED FLUORESCENT REAGENTS
    5.
    发明申请
    MULTIMERIC PROTECTED FLUORESCENT REAGENTS 审中-公开

    公开(公告)号：US20190309171A1

    公开(公告)日：2019-10-10

    申请号：US16209916

    申请日：2018-12-04

    申请人： Pacific Biosciences of California, Inc.

    发明人： Frank ZHENG ,  Jeremiah HANES ,  Gene SHEN ,  Louis BROGLEY ,  Stephen YUE ,  Yuri LAPIN ,  John LYLE ,  Honey OSUNA ,  Andrei FEDOROV ,  Lubomir SEBO

    IPC分类号： C09B69/10 ,  G01N33/533 ,  C09B23/10 ,  C09B23/06 ,  C09B69/00

    摘要： Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The reagents contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements. The reagents also contain coupling elements connect monomeric compounds into multimeric forms, thereby increasing brightness.

    PROTECTED FLUORESCENT REAGENT COMPOUNDS
    6.
    发明申请
    PROTECTED FLUORESCENT REAGENT COMPOUNDS 有权
    保护荧光试剂化合物

    公开(公告)号：US20150050659A1

    公开(公告)日：2015-02-19

    申请号：US14452497

    申请日：2014-08-05

    申请人： Pacific Biosciences of California, Inc.

    发明人： Lubomir SEBO ,  Jeremiah HANES ,  Gene SHEN ,  Louis BROGLEY ,  Stephen YUE ,  Frank ZHENG ,  Yuri LAPIN ,  John LYLE ,  Honey OSUNA ,  Andrei FEDOROV

    IPC分类号： C12Q1/68 ,  C07H19/20 ,  C07F9/655

    CPC分类号： C07H19/10 ,  C07F9/65586 ,  C07F9/65616 ,  C09B23/06 ,  C09B23/083 ,  C09B69/105

    摘要： Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

    摘要翻译： 提供了受保护的荧光试剂化合物及其合成方法。 该化合物可用于各种基于荧光的分析方法，包括以高密度大量多重光学反应的分析，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，其允许以期望波长的高灵敏度检测化合物，允许化合物被目标生物分子特异性识别的结合元件以及减少荧光染料元件之间的不期望接触的保护屏蔽元件 和结合的靶生物分子，因此由荧光染料元素降低结合的靶生物分子的光损伤。

    MODIFIED NUCLEOTIDE REAGENTS
    8.
    发明申请
    MODIFIED NUCLEOTIDE REAGENTS 审中-公开

    公开(公告)号：US20200087722A1

    公开(公告)日：2020-03-19

    申请号：US16440913

    申请日：2019-06-13

    申请人： Pacific Biosciences of California, Inc.

    发明人： Gene SHEN ,  Stephen YUE ,  Lubomir SEBO ,  Louis BROGLEY

    IPC分类号： C12Q1/6869 ,  C07H21/04 ,  C07H19/207 ,  C07H19/052 ,  C12Q1/6876 ,  C09B23/08 ,  C09B23/06 ,  C09B69/00

    摘要： Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

    MODIFIED NUCLEOTIDE REAGENTS
    9.
    发明申请
    MODIFIED NUCLEOTIDE REAGENTS 审中-公开

    公开(公告)号：US20170145502A1

    公开(公告)日：2017-05-25

    申请号：US15357958

    申请日：2016-11-21

    申请人： Pacific Biosciences of California, Inc.

    发明人： Gene SHEN ,  Stephen YUE ,  Lubomir SEBO ,  Louis BROGLEY

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C07H19/10 ,  C07H19/207 ,  C09B69/105 ,  C12Q1/6869

    摘要： Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.

    PROTECTED DYE-LABELED REAGENTS
    10.
    发明公开
    PROTECTED DYE-LABELED REAGENTS 审中-公开

    公开(公告)号：US20240158640A1

    公开(公告)日：2024-05-16

    申请号：US18423259

    申请日：2024-01-25

    申请人： Pacific Biosciences of California, Inc.

    发明人： Lubomir SEBO ,  Honey OSUNA ,  Stephen YUE ,  Yuri LAPIN

    IPC分类号： C09B69/10 ,  C07D495/04 ,  C07H19/10 ,  C07H19/207 ,  C12Q1/6869 ,  G01N21/64

    CPC分类号： C09B69/105 ,  C07D495/04 ,  C07H19/10 ,  C07H19/207 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6439

    摘要： Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.