Detection device and detection method

    公开(公告)号:US11768155B2

    公开(公告)日:2023-09-26

    申请号:US16747021

    申请日:2020-01-20

    CPC classification number: G01N21/648 G01N33/54373 G01N33/6857 G01N2021/6441

    Abstract: The present disclosure provides a detection device capable of detecting a low concentration of an analyte with high sensitivity. The detection apparatus according to the present disclosure comprises a metal microstructure on which a first VHH antibody having a property of binding specifically to the analyte is immobilized and which generate surface plasmon by being irradiated with excitation light, an inlet through which a second VHH antibody and a sample that may contain an analyte are introduced, wherein the second VHH antibody has a property of binding specifically to the analyte and is labeled with a fluorescent substance, a light source for irradiating the metal microstructure to which the second VHH antibody and the sample have been introduced with the excitation light, and a detection unit for detecting the analyte on the basis of fluorescence generated from the fluorescent substance by the irradiation of the excitation light.

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