Screen for CDC7 inhibitors
    3.
    发明授权
    Screen for CDC7 inhibitors 失效
    筛选CDC7抑制剂

    公开(公告)号:US06953672B2

    公开(公告)日:2005-10-11

    申请号:US10225323

    申请日:2002-08-21

    CPC分类号: C07K14/4738

    摘要: Disclosed is a yeast cell whose genetic complement includes an inactive allele of the yeast CDC7 gene, a first nucleic acid that encodes a mammalian Cdc7 protein, and a second nucleic acid that encodes a mammalian Dbf4 protein. The yeast cell is dependent on the mammalian Cdc7 and Dbf4 proteins for viability. The yeast cell can be used to identify potential anti-proliferative agents by virtue of their inhibition of the mammalian Cdc7 and Dbf4 proteins. In some embodiments, a control yeast cell which does not depend on Cdc7 for viability is used in a secondary screen.

    摘要翻译: 公开了酵母细胞,其遗传补体包括酵母CDC7基因的无活性等位基因,编码哺乳动物Cdc7蛋白的第一核酸和编码哺乳动物Dbf4蛋白的第二核酸。 酵母细胞依赖于哺乳动物Cdc7和Dbf4蛋白的活力。 酵母细胞可以通过抑制哺乳动物Cdc7和Dbf4蛋白来鉴定潜在的抗增殖剂。 在一些实施方案中,在二次屏幕中使用不依赖Cdc7作为存活力的对照酵母细胞。