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公开(公告)号：US07300777B2
公开(公告)日：2007-11-27
申请号：US11136887
申请日：2005-05-25
IPC分类号： C12P13/08 , C12P13/24 , C12P13/14 , C12P13/12 , C12P13/10 , C12P13/06 , C07H21/04 , C12N9/00
CPC分类号： C12N9/93 , C12P13/08 , C12Y604/01001
摘要： The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.
摘要翻译： 本发明涉及来自棒状杆菌的突变型丙酮酸羧化酶基因。 突变体丙酮酸羧化酶基因编码丙酮酸羧化酶，其对天冬氨酸的反馈抑制具有抗性。 本发明还涉及用该反馈抗性丙酮酸羧化酶基因替代棒状杆菌中的野生型丙酮酸羧化酶基因的方法。 本发明还涉及生产氨基酸，优选赖氨酸的方法，其包括在微生物中使用该突变型丙酮酸羧化酶。
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公开(公告)号：US06451564B1
公开(公告)日：2002-09-17
申请号：US09608065
申请日：2000-06-30
IPC分类号： C12P1306
CPC分类号： C12P13/06 , C12N9/88 , C12Y403/01019
摘要： The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms. More specifically, the present invention relates to methods for producing L-isoleucine comprising: (a) growing a transformed nonhuman organism under conditions that provide for synthesis of L-isoleucine, wherein the nonhuman organism comprises one or more copies of a transgene comprising at least one nucleotide sequence encoding catabolic threonine dehydratase; wherein the L-isoleucine is synthesized by the transformed nonhuman organism, the synthesis being greater than that of the corresponding non-transformed nonhuman organism; and (b) recovering the L-isoleucine from the culture medium in which the transformed nonhuman organism was cultured.
摘要翻译： 本发明一般涉及非人类生物体过量生产L-异亮氨酸。 更具体地说，本发明涉及生产L-异亮氨酸的方法，其包括：（a）在提供L-异亮氨酸合成的条件下生长转化的非人生物，其中所述非人生物体包含至少一个或多个转基因 编码分解代谢苏氨酸脱水酶的一个核苷酸序列; 其中所述L-异亮氨酸由所述转化的非人生物合成，所述合成大于相应的未转化的非人生物的合成; 和（b）从其中培养转化的非人生物的培养基中回收L-异亮氨酸。
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公开(公告)号：US06987017B2
公开(公告)日：2006-01-17
申请号：US10223644
申请日：2002-08-20
IPC分类号： C12N1/00
CPC分类号： C12P13/06 , C12N9/88 , C12Y403/01019
摘要： The present invention relates, in general, to the over-production of L-isoleucine by nonhuman organisms. More specifically, the present invention relates to methods for producing L-isoleucine comprising: (a) growing a transformed nonhuman organism under conditions that provide for synthesis of L-isoleucine, wherein the nonhuman organism comprises one or more copies of a transgene comprising at least one nucleotide sequence encoding catabolic threonine dehydratase; wherein the L-isoleucine is synthesized by the transformed nonhuman organism, the synthesis being greater than that of the corresponding non-transformed nonhuman organism; and (b) recovering the L-isoleucine from the culture medium in which the transformed nonhuman organism was cultured.
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公开(公告)号：US20050221452A1
公开(公告)日：2005-10-06
申请号：US11136887
申请日：2005-05-25
申请人： Paul Hanke , Anthony Sinskey , Laura Willis , Stephane Guillouet
发明人： Paul Hanke , Anthony Sinskey , Laura Willis , Stephane Guillouet
CPC分类号： C12N9/93 , C12P13/08 , C12Y604/01001
摘要： The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.
摘要翻译： 本发明涉及来自棒状杆菌的突变型丙酮酸羧化酶基因。 突变体丙酮酸羧化酶基因编码丙酮酸羧化酶，其对天冬氨酸的反馈抑制具有抗性。 本发明还涉及用该反馈抗性丙酮酸羧化酶基因替代棒状杆菌中的野生型丙酮酸羧化酶基因的方法。 本发明还涉及生产氨基酸，优选赖氨酸的方法，其包括在微生物中使用该突变型丙酮酸羧化酶。
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公开(公告)号：US20120295319A1
公开(公告)日：2012-11-22
申请号：US12740295
申请日：2008-10-29
CPC分类号： C12P7/06 , C12N9/0006 , C12Y101/01008 , C12Y101/05003 , Y02E50/17
摘要： The invention relates to a method of modifying a yeast cell for the production of ethanol. According to the invention, the activity of the Gpd1 protein and/or the Gpd2 protein is reduced.
摘要翻译： 本发明涉及一种修饰用于生产乙醇的酵母细胞的方法。 根据本发明，Gpd1蛋白和/或Gpd2蛋白的活性降低。
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公开(公告)号：US09175270B2
公开(公告)日：2015-11-03
申请号：US12740295
申请日：2008-10-29
IPC分类号： C12N9/04
CPC分类号： C12P7/06 , C12N9/0006 , C12Y101/01008 , C12Y101/05003 , Y02E50/17
摘要： The invention relates to a method of modifying a yeast cell for the production of ethanol. According to some embodiments of the invention, the activity of the Gpd1 protein and/or the Gpd2 protein is reduced.
摘要翻译： 本发明涉及一种修饰用于生产乙醇的酵母细胞的方法。 根据本发明的一些实施方案，Gpd1蛋白和/或Gpd2蛋白的活性降低。
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