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公开(公告)号：US12108771B2
公开(公告)日：2024-10-08
申请号：US17321382
申请日：2021-05-14
发明人： Anthony J. Sinskey , ChoKyun Rha , T. G. Sambandan , Ravigadevi Sambanthamurthi , Anastasia Artamonova , Joseph De Angelo , Kevin Ohashi
IPC分类号： A23F5/46 , A23L27/00 , A23L27/12 , A23L33/105 , A61K36/889
CPC分类号： A23F5/465 , A23L27/12 , A23L27/88 , A23L33/105 , A61K36/889 , A23V2002/00
摘要： The invention provides a method for enhancing flavour profile of beverages comprising increasing perceptibility of sweetness, increasing acidity, and decreasing astringent taste in beverages, increasing perceptibility of floral and spice notes by adding an effective flavour enhancing amount of water-soluble Palm Fruit Bioactive complex (wsPFBc/OPP) or their extracts into a beverage is disclosed a composition comprising a beverage and oil palm phenolics (OPP) or their extracts such that the OPP or their extracts modify or alter the fragrance and flavour of the beverage.
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2.发明申请COMPOSITIONS COMPRISING SHIKIMIC ACID OBTAINED FROM OIL PALM BASED MATERIALS AND METHOD OF PRODUCING THEREOF 审中-公开包含从基于棕榈的材料获得的活性酸的组合物及其生产方法公开(公告)号：US20130211136A1
公开(公告)日：2013-08-15
申请号：US13704616
申请日：2011-06-16
申请人： Sambandan Tg , ChoKyun Rha , Ravigadevi Sambanthamurthi , Anthony J. Sinskey , Yew Ai Tan , Kalyana Sundram P. Manickam , Mohd Basri Wahid
发明人： Sambandan Tg , ChoKyun Rha , Ravigadevi Sambanthamurthi , Anthony J. Sinskey , Yew Ai Tan , Kalyana Sundram P. Manickam , Mohd Basri Wahid
IPC分类号： C07C51/42
CPC分类号： C07C51/50 , C07C51/42 , C07C51/48 , C07C2601/16 , C12N9/1092 , C12Y205/01019 , C07C62/32
摘要： The present invention provides compositions and method for production of shikimic acid based on extracts obtained from oil palm-based materials, and more particularly oil palm based waste materials and by-products. The method includes purifying shikimic acid from extracts comprising oil palm phenolics (OPP).
摘要翻译： 本发明提供了基于由油棕基材料提取的提取物，特别是油棕基废料和副产物的莽草酸生产组合物和方法。 该方法包括从包含油棕榈酚（OPP）的提取物中纯化莽草酸。
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公开(公告)号：US06528706B1
公开(公告)日：2003-03-04
申请号：US09935244
申请日：2001-08-21
IPC分类号： A01H500
CPC分类号： C12N9/0006 , C12N9/00 , C12N9/1029 , C12N15/52 , C12P7/625
摘要： A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.
摘要翻译： 通过在原核和真核细胞，特别是植物中的分子水平处理聚羟基丁酸酯（PHB）和聚羟基链烷酸酯（PHA）聚酯的遗传学和酶学方法来控制和改变生物聚合物合成的方法。 实施例证明参与PHB和PHA聚合物生产的基因的分离，表征和表达。 鉴定或分离来自Zoogloea苎麻菌株I-16-M，产碱假单胞菌，痢疾杆菌（Nocardia salmonicolur）和橄榄油（Psuedomonas olevarans）的PHB和PHA合成途径（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB聚合酶或PHA聚合酶）中的酶编码基因 并在非PHB生物体大肠杆菌中表达。 对聚合物的具体修饰包括聚合物链长度的变化以及将不同单体引入到聚合物中以产生具有不同物理性质的共聚物。
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公开(公告)号：US5661026A
公开(公告)日：1997-08-26
申请号：US435083
申请日：1995-05-04
CPC分类号： C12N9/0006 , C12N15/52 , C12N9/00 , C12N9/1029 , C12P7/625
摘要： The present invention is a method for controlling biopolymer synthesis by determining the genetics and enzymology of polyhydroxybutyrate (PHB) biosynthesis at the molecular level. The purified enzymes and genes provide the means for developing new PHB-like biopolymers having polyester backbones. Specific aims are to 1) control the chain length of the polymers produced in fermentation processes through genetic manipulation, 2) incorporate different monomers into the polymers to produce co-polymers with different physical properties, and 3) examine the physical/rheological properties of these new biopolymers in order to develop further design criteria at the molecular level.The method for engineering biopolymer synthesis includes: isolation and characterization of the genes for the enzymes in the synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB synthetase); cloning of the genes in a vector(s); placement of the vector(s) under the control of regulated promoters; expression of the genes; determination of the function and use of other factors such as substrate specificity in polymer production and composition; and isolation and physical and chemical analysis of the resulting polymers.
摘要翻译： 本发明是通过在分子水平上测定聚羟基丁酸（PHB）生物合成的遗传学和酶学方法来控制生物聚合物合成的方法。 纯化的酶和基因为开发具有聚酯主链的新型PHB样生物聚合物提供了手段。 具体目的是1）通过遗传操作来控制在发酵过程中产生的聚合物的链长; 2）将不同的单体引入到聚合物中以产生具有不同物理性质的共聚物，以及3）检查这些物质/流变性质 新的生物聚合物，以便在分子水平上制定进一步的设计标准。 工程生物聚合物合成的方法包括：合成途径中酶的分离和表征（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB合成酶）; 在载体中克隆基因; 将载体置于受调节启动子的控制下; 基因表达; 确定聚合物生产和组成中其他因素如底物特异性的功能和用途; 以及所得聚合物的分离和物理和化学分析。
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公开(公告)号：US5512669A
公开(公告)日：1996-04-30
申请号：US297667
申请日：1994-08-29
CPC分类号： C12N9/0006 , C12N15/52 , C12N9/00 , C12N9/1029 , C12P7/625
摘要： The present invention is a method for controlling biopolymer synthesis by determining the genetics and enzymology of polyhydroxybutyrate (PHB) biosynthesis at the molecular level. The purified enzymes and genes provide the means for developing new PHB-like bicpolymers having polyester backbones. Specific aims are to 1) control the chain length of the polymers produced in fermentation processes through genetic manipulation, 2) incorporate different monomers into the polymers to produce co-polymers with different physical properties, and 3) examine the physical/rheological properties of these new biopolymers in order to develop further design criteria at the molecular level.The method for engineering biopolymer synthesis includes: isolation and characterization of the genes for the enzymes in the synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB synthetase); cloning of the genes in a vector(s); placement of the vector(s) under the control of regulated promoters; expression of the genes; determination of the function and use of other factors such as substrate specificity in polymer production and composition; and isolation and physical and chemical analysis of the resulting polymers.
摘要翻译： 本发明是通过在分子水平上测定聚羟基丁酸（PHB）生物合成的遗传学和酶学方法来控制生物聚合物合成的方法。 纯化的酶和基因提供了开发具有聚酯主链的新型PHB-类双聚物的手段。 具体目的是1）通过遗传操作来控制在发酵过程中产生的聚合物的链长; 2）将不同的单体引入到聚合物中以产生具有不同物理性质的共聚物，以及3）检查这些物质/流变性质 新的生物聚合物，以便在分子水平上制定进一步的设计标准。 工程生物聚合物合成的方法包括：合成途径中酶的分离和表征（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB合成酶）; 在载体中克隆基因; 将载体置于受调节启动子的控制下; 基因表达; 确定聚合物生产和组成中其他因素如底物特异性的功能和用途; 以及所得聚合物的分离和物理和化学分析。
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公开(公告)号：US5506124A
公开(公告)日：1996-04-09
申请号：US107497
申请日：1993-08-17
申请人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
发明人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
CPC分类号： C12R1/865 , C08B37/0024 , C09K3/00 , C12P19/04 , Y10S435/942
摘要： Three dimensional glucan matrix compositions are prepared by separating growing yeast from its growth medium, subjecting the yeast with cell walls intact to an alkali material, thereby extracting whole glucan particles having an intact cell wall structure. The whole glucans can then, optionally, be treated with acetic acid to alter the .beta.(1-6) linkages, or with glucanase to alter the .beta.(1-3) linkages. The glucans have viscosity characteristics dependent upon the strain of yeast utilized and are useful as stabilizers or thickeners.
摘要翻译： 通过从其生长培养基中分离生长的酵母，将酵母细胞壁完整地进行碱性物质制备三维葡聚糖基质组合物，从而提取具有完整细胞壁结构的全部葡聚糖颗粒。 然后可以任选地用乙酸处理整个葡聚糖以改变β（1-6）键或用葡聚糖酶改变β（1-3）键。 葡聚糖具有取决于所用酵母的菌株的粘度特性，并且可用作稳定剂或增稠剂。
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公开(公告)号：US4992540A
公开(公告)日：1991-02-12
申请号：US297982
申请日：1989-01-17
申请人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
发明人： Spiros Jamas , ChoKyun Rha , Anthony J. Sinskey
CPC分类号： C12P19/04 , C08B37/0024 , C09K3/00
摘要： Three dimensional glucan matrix compositions are prepared by separating growing yeast from its growth medium, subjecting the yeast with cell walls intact to an alkali material, thereby extracting whole glucan particles having an intact cell wall structure. The whole glucans can then, optionally, be treated with acetic acid to alter the .beta.(1-6) linkages, or with glucanase to alter the .beta.(1-3) linkages. The glucans have viscosity characteristics dependent upon the strain of yeast utilized and are useful as stabilizers or thickeners.
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8.发明授权Zoogloea transformation using exopoly saccharide non-capsule producing strains 失效Zoogloea转化使用外源糖非胶囊生产菌株
公开(公告)号：US4948733A
公开(公告)日：1990-08-14
申请号：US35604
申请日：1987-04-07
摘要： Two new bacterial strains designated Zoogloea ramigera 115SL and Zoogloea ramigera 115SLR, a rifampicin resistant derivative of 115SL, have been developed. These strains are derived from the wild type Zoogloea ramigera 115, ATCC 25935. The two new strains produce a novel exopolysaccharide (EPS) and have several desirable characteristics that are absent from the parent strain, including improved culture properties, since they do not produce an EPS capsule layer like that of the parent 115 strain. The 115SL EPS is instead excreted as a slime layer which is not confined to the immediate area surrounding the cells. Since cells are not trapped within a floc where they grow at a reduced rate or die because of nutrient starvation, the new strains have more consistent and reproducible growth cycles and increased growth rates. As a consequence, exopolysaccharide production is more consistent and titers are higher. The separation of the EPS from the cells is also much easier and more economical. The other very important characteristic of strains 115SL and 115SLR is that they are able to receive foreign DNA using conventional techniques due to the absence of the capsule layer. This facilitates the application of recombinant DNA technology to control and produce novel expolysaccharides.
摘要翻译： 已经开发了两种新的细菌菌株，称为Zoogloea ramigera 115SL和Zoogloea ramigera 115SLR，115SL的利福平抗性衍生物。 这些菌株衍生自野生型Zoogloea ramigera 115，ATCC 25935.两种新菌株产生新颖的外多糖（EPS），并具有几种所需的特征，其不存在于亲本菌株中，包括改良的培养性质，因为它们不产生 EPS胶囊层像母体115株。 相反，115SL EPS排泄成为不限于细胞周围的紧邻区域的粘液层。 由于细胞不被捕获在絮状物中，它们以降低的速率生长或由于营养物质饥饿而死亡，所以新菌株具有更一致和可重现的生长周期和增加的生长速率。 结果，外多糖生产更一致，滴度更高。 EPS与细胞的分离也更容易和更经济。 菌株115SL和115SLR的另一个非常重要的特征是，由于不存在胶囊层，它们能够使用常规技术接收外源DNA。 这有利于重组DNA技术的应用来控制和生产新的多糖。
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9.发明申请COMPOSITION COMPRISING CAFFEOYLSHIKIMIC ACIDS, PROTOCATECHUIC ACID, HYDROXYTYROSOL, HYDROXYBENZOIC ACID AND THEIR DERIVATIVES AND METHOD OF PREPARATION THEREOF 审中-公开包含CAFFEOYLSHIICIC酸，丙酸酸，羟基苯甲酸，羟基苯甲酸及其衍生物的组合物及其制备方法公开(公告)号：US20120238773A1
公开(公告)日：2012-09-20
申请号：US13322494
申请日：2010-05-26
申请人： T.G. Sambandan , ChoKyun Rha , Anthony J. Sinskey , Ravigadevi Sambanthamurthi , Yew Ai Tan , Kalyana Sundram P. Manickam , Mohd Basri Wahid
发明人： T.G. Sambandan , ChoKyun Rha , Anthony J. Sinskey , Ravigadevi Sambanthamurthi , Yew Ai Tan , Kalyana Sundram P. Manickam , Mohd Basri Wahid
IPC分类号： C07C69/732 , C07C67/48
CPC分类号： A61K36/889
摘要： A composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives extracted from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent, and a method for use in the preparation of a composition containing caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives. The method includes the steps of pre-concentrating an extract containing the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives and isolating the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives from said extract by liquid chromatography, wherein the elution activity of said caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, with the caffeoylshikimic acids and their derivatives varying depending on the stationary phase and the composition of the mobile phase.
摘要翻译： 一种组合物，其包含咖啡酰莽卡木酸，原儿茶酸，羟基酪醇，羟基苯甲酸，所述咖啡酰基莽草酸及其衍生物从油棕的任何部分提取，包括但不限于棕榈油研磨和棕榈油磨出料的植物液，以及使用方法 在制备含有咖啡酰基莽草酸，原儿茶酸，羟基酪醇，羟基苯甲酸，所述咖啡酰莽卡米酸及其衍生物的组合物。 该方法包括以下步骤：将含有咖啡酰基莽草酸，原儿茶酸，羟基酪醇，羟基苯甲酸，咖啡酰基莽草酸及其衍生物的提取物预浓缩，并分离出咖啡酰基莽草酸，原儿茶酸，羟基酪醇，羟基苯甲酸，所述咖啡酰莽卡米酸及其衍生物 所述提取物通过液相色谱法，其中所述咖啡酰基莽草酸，原儿茶酸，羟基酪醇，羟基苯甲酸与咖啡酰基莽草酸及其衍生物的洗脱活性根据固定相和流动相的组成而变化。
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公开(公告)号：US07300777B2
公开(公告)日：2007-11-27
申请号：US11136887
申请日：2005-05-25
IPC分类号： C12P13/08 , C12P13/24 , C12P13/14 , C12P13/12 , C12P13/10 , C12P13/06 , C07H21/04 , C12N9/00
CPC分类号： C12N9/93 , C12P13/08 , C12Y604/01001
摘要： The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.
摘要翻译： 本发明涉及来自棒状杆菌的突变型丙酮酸羧化酶基因。 突变体丙酮酸羧化酶基因编码丙酮酸羧化酶，其对天冬氨酸的反馈抑制具有抗性。 本发明还涉及用该反馈抗性丙酮酸羧化酶基因替代棒状杆菌中的野生型丙酮酸羧化酶基因的方法。 本发明还涉及生产氨基酸，优选赖氨酸的方法，其包括在微生物中使用该突变型丙酮酸羧化酶。
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