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    Methods for producing self-replicating infectious RSV particles comprising recombinant RSV genomes or antigenomes and the N, P, L, and M2 proteins
    5.
    发明授权
    Methods for producing self-replicating infectious RSV particles comprising recombinant RSV genomes or antigenomes and the N, P, L, and M2 proteins 有权
    用于产生包含重组RSV基因组或抗原单核细胞和N，P，L和M2蛋白质的自复制感染性RSV颗粒的方法

    公开(公告)号：US06790449B2

    公开(公告)日：2004-09-14

    申请号：US09847173

    申请日：2001-05-03

    申请人： Peter L. Collins

    发明人： Peter L. Collins

    IPC分类号： A61K39155

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/525 ,  C12N7/00 ,  C12N15/86 ,  C12N2760/18521 ,  C12N2760/18522 ,  C12N2760/18543

    摘要： RNA synthesis by the paramyxovirus respiratory syncytial virus (RSV), a ubiquitous human pathogen, was found to be more complex than previously appreciated for the nonsegmented negative-strand RNA viruses. Intracellular RNA replication of a plasmid-encoded “minigenome” analog of viral genomic RNA was directed by coexpression of the nucleocapsid (N) protein, nucleocapsid phosphoprotein (P), and the large polymerase (L) protein. But, under these conditions, it appeared that the greater part of mRNA synthesis terminated prematurely. However, coexpression of the M2 (ORF1) gene resulted in the efficient production of full-length mRNA. Thus, these results demonstrate that expression of the upstream ORF1, which encoded the previously described 22-kDa M2 protein, was associated with transcription elongation. Accordingly, the claimed invention is directed toward infectious recombinant RSV particles which comprise a recombinant genome or antigenome, as well as the RSV proteins N, P, L, and the RNA polymerase elongation factor M2. This system will permit the introduction of defined changes into infectious RSV which will prove useful in a variety of applications, such as the analysis of RSV molecular biology and pathogenesis, the development of attenuated RSV immunogenic compositions for the preparation of RSV-specific immunological reagents, and the expression of foreign antigens.

    摘要翻译： 发现通过普遍存在的人类病原体的副粘病毒呼吸道合胞病毒（RSV）的RNA合成比以前对非分段负链RNA病毒所认识的更为复杂。 病毒基因组RNA的质粒编码的“小基因组”类似物的细胞内RNA复制通过核衣壳（N）蛋白，核壳蛋白磷蛋白（P）和大聚合酶（L）蛋白的共表达来引导。 但是，在这些条件下，mRNA合成的较大部分似乎过早终止。 然而，M2（ORF1）基因的共表达导致全长mRNA的有效产生。 因此，这些结果表明编码前述的22-kDa M2蛋白的上游ORF1的表达与转录延伸有关。 因此，要求保护的发明涉及包含重组基因组或反向原子团以及RSV蛋白N，P，L和RNA聚合酶延伸因子M2的感染性重组RSV颗粒。 该系统将允许将感染性RSV引入定义的变化，这将在各种应用中有用，例如RSV分子生物学和发病机理的分析，用于制备RSV特异性免疫试剂的减毒RSV免疫原性组合物的开发， 和外源抗原的表达。

    Human respiratory virus preparahons and processess
    6.
    发明授权
    Human respiratory virus preparahons and processess 失效
    人类呼吸道病毒预处理和过程

    公开(公告)号：US5716823A

    公开(公告)日：1998-02-10

    申请号：US854783

    申请日：1997-05-12

    申请人： Gail W. Wertz ,  Peter L. Collins

    发明人： Gail W. Wertz ,  Peter L. Collins

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/155 ,  C07K14/135 ,  C12N15/00 ,  C12N1/12 ,  C12N1/20 ,  C12N7/00

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/155 ,  A61K38/00 ,  A61K39/00 ,  C12N2760/18522 ,  C12N2760/18534

    摘要： This invention discloses compositions of DNA and proteins that are useful for preparing vaccines against human respiratory syncytial virus �HRSV!. The DNA compositions include structural genes coding for native structural viral proteins and immunogenic fragments of these proteins. Host cells transformed with the above DNA compositions are also disclosed. Vaccines made from the native structural viral proteins or immunogenic fragments are also disclosed as well as methods for protecting humans by inoculation with these vaccines.

    摘要翻译： 本发明公开了可用于制备针对人呼吸道合胞病毒（HRSV）的疫苗的DNA和蛋白质组合物。 DNA组合物包括编码天然结构病毒蛋白的结构基因和这些蛋白质的免疫原性片段。 还公开了用上述DNA组合物转化的宿主细胞。 还公开了由天然结构病毒蛋白质或免疫原性片段制备的疫苗以及通过接种这些疫苗来保护人的方法。

    Vaccines for human respiratory virus
    7.
    发明授权
    Vaccines for human respiratory virus 失效
    人类呼吸道病毒疫苗

    公开(公告)号：US5149650A

    公开(公告)日：1992-09-22

    申请号：US218737

    申请日：1988-07-13

    申请人： Gail W. Wertz ,  Peter L. Collins

    发明人： Gail W. Wertz ,  Peter L. Collins

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/155 ,  C07K14/135

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/155 ,  A61K38/00 ,  A61K39/00 ,  C12N2760/18522 ,  C12N2760/18534

    摘要： This invention discloses compositions of DNA and proteins that are useful for preparing vaccines against human respiratory syncytial virus [HRSV]. The DNA compositions include structural genes coding for native structural viral proteins and immunogenic fragments of these proteins. Host cells transformed with the above DNA compositions are also disclosed. Vaccines made from the native structural viral proteins or immunogenic fragments are also disclosed as well as methods for protecting humans by inoculation with these vaccines.

    摘要翻译： 本发明公开了可用于制备针对人呼吸道合胞病毒（HRSV）的疫苗的DNA和蛋白质组合物。 DNA组合物包括编码天然结构病毒蛋白的结构基因和这些蛋白质的免疫原性片段。 还公开了用上述DNA组合物转化的宿主细胞。 还公开了由天然结构病毒蛋白质或免疫原性片段制备的疫苗以及通过接种这些疫苗来保护人的方法。