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公开(公告)号：US06677121B2
公开(公告)日：2004-01-13
申请号：US09855793
申请日：2001-05-15
申请人： Paul M. Lizardi , Matthew E. Roth , Li Feng , Cesar E. Guerra , Shane C. Weber , Joseph C. Kaufman , Darin R. Latimer
发明人： Paul M. Lizardi , Matthew E. Roth , Li Feng , Cesar E. Guerra , Shane C. Weber , Joseph C. Kaufman , Darin R. Latimer
IPC分类号： C12Q168
CPC分类号： C12Q1/6855 , C12Q1/6809 , C12Q1/6837 , Y10S977/924 , C12Q2525/191 , C12Q2525/179 , C12Q2521/313 , C12Q2565/513
摘要： Disclosed is a method for the comprehensive analysis of nucleic acid samples and a detector composition for use in the method. The method, referred to as Fixed Address Analysis of Sequence Tags (FAAST), involves generation of a set of nucleic acid fragments having a variety of sticky end sequences; indexing of the fragments into sets based on the sequence of sticky ends; associating a detector sequence with the fragments; sequence-based capture of the indexed fragments on a detector array; and detection of the fragment labels. Generation of the multiple sticky end sequences is accomplished by incubating the nucleic acid sample with one or more nucleic acid cleaving reagents. The indexed fragments are captured by hybridization and coupling, preferably by ligation, to a probe. The method allows a complex sample of nucleic acid to be quickly and easily cataloged in a reproducible and sequence-specific manner. One form of the method allows determination of associations, in a nucleic acid molecule, of different combinations of known or potential sequences. Another form of the method assesses modification of sequences in nucleic acid molecules by basing cleavage of the molecules on the presence or absence of modification.
摘要翻译： 公开了用于综合分析核酸样品的方法和用于该方法的检测器组合物。 称为序列标签的固定地址分析（FAAST）的方法涉及产生具有各种粘性末端序列的一组核酸片段; 基于粘性末端的顺序将片段索引到集合中; 将检测器序列与片段相关联; 检测器阵列上索引片段的基于序列的捕获; 并检测片段标签。 通过将核酸样品与一种或多种核酸切割试剂孵育来实现多粘性末端序列的产生。 索引的片段通过杂交和偶联，优选通过连接被捕获到探针。 该方法允许复制的核酸样品以可再现和序列特异性方式快速且容易地编目。 该方法的一种形式允许确定核酸分子中已知或潜在序列的不同组合的缔合。 该方法的另一种形式通过在存在或不存在修饰的基础上分解裂解来评估核酸分子中序列的修饰。
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公开(公告)号：US06261782B1
公开(公告)日：2001-07-17
申请号：US09544713
申请日：2000-04-06
申请人： Paul M. Lizardi , Matthew E. Roth , Li Feng , Cesar E. Guerra , Shane C. Weber , Joseph C. Kaufman , Darin R. Latimer
发明人： Paul M. Lizardi , Matthew E. Roth , Li Feng , Cesar E. Guerra , Shane C. Weber , Joseph C. Kaufman , Darin R. Latimer
IPC分类号： C12Q168
CPC分类号： C12Q1/6855 , C12Q1/6809 , C12Q1/6837 , Y10S977/924 , C12Q2525/191 , C12Q2525/179 , C12Q2521/313 , C12Q2565/513
摘要： Disclosed is a method for the comprehensive analysis of nucleic acid samples and a detector composition for use in the method. The method, referred to as Fixed Address Analysis of Sequence Tags (FAAST), involves generation of a set of nucleic acid fragments having a variety of sticky end sequences; indexing of the fragments into sets based on the sequence of sticky ends; associating a detector sequence with the fragments; sequence-based capture of the indexed fragments on a detector array; and detection of the fragment labels. Generation of the multiple sticky end sequences is accomplished by incubating the nucleic acid sample with one or more nucleic acid cleaving reagents. The indexed fragments are captured by hybridization and coupling, preferably by ligation, to a probe. The method allows a complex sample of nucleic acid to be quickly and easily cataloged in a reproducible and sequence-specific manner. One form of the method allows determination of associations, in a nucleic acid molecule, of different combinations of known or potential sequences. Another form of the method assesses modification of sequences in nucleic acid molecules by basing cleavage of the molecules on the presence or absence of modification.
摘要翻译： 公开了用于综合分析核酸样品的方法和用于该方法的检测器组合物。 称为序列标签的固定地址分析（FAAST）的方法涉及产生具有各种粘性末端序列的一组核酸片段; 基于粘性末端的序列将片段索引到集合中; 将检测器序列与片段相关联; 检测器阵列上索引片段的基于序列的捕获; 并检测片段标签。 通过将核酸样品与一种或多种核酸切割试剂孵育来实现多粘性末端序列的产生。 索引的片段通过杂交和偶联，优选通过连接被捕获到探针。 该方法允许复制的核酸样品以可再现和序列特异性方式快速且容易地编目。 该方法的一种形式允许确定核酸分子中已知或潜在序列的不同组合的缔合。 该方法的另一种形式通过在存在或不存在修饰的基础上分解裂解来评估核酸分子中序列的修饰。
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3.发明授权Silver halide emulsions, elements and methods of making same using synthetic biopolymer peptizers 失效卤化银乳剂，使用合成生物聚合物胶溶剂制备其的元素和方法
公开(公告)号：US5580712A
公开(公告)日：1996-12-03
申请号：US383348
申请日：1995-02-03
CPC分类号： G03C1/0051 , C07K14/78 , G03C1/047 , G03C1/015
摘要： Certain synthetically prepared biopolymers are useful as peptizers in the preparation of photographic silver halide emulsions and elements. Such materials can be used as either nucleation or growth peptizers in place of common peptizing colloids, such as gelatins. The biopolymers can be prepared using recombinant or chemical synthetic methods and designed to have a particular affinity (either high or low) for silver ions. Thus, they can be used to control silver halide grain morphology in the emulsions.
摘要翻译： 某些合成制备的生物聚合物可用作制备照相卤化银乳剂和元素的胶溶剂。 这种材料可以用作成核或生长胶溶剂代替普通的胶溶胶体，例如明胶。 生物聚合物可以使用重组或化学合成方法制备，并设计为对银离子具有特殊的亲和力（高或低）。 因此，它们可用于控制乳液中的卤化银颗粒形态。
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4.发明授权Preparation of thin tabular grain silver halide emulsions using synthetic polymeric peptizers 失效使用合成聚合物胶溶剂制备薄的片状银卤化银乳剂
公开(公告)号：US5385819A
公开(公告)日：1995-01-31
申请号：US171588
申请日：1993-12-22
CPC分类号： G03C1/053 , G03C1/0051
摘要： A process for preparing a thin tabular grain silver halide emulsion comprised of silver halide grains which have a halide content of at least 50 mole percent bromide, wherein tabular grains of less than 0.15 micrometers in thickness and having an aspect ratio of greater than 8 account for greater than 50 percent of the total grain projected area, comprises the steps of nucleating the silver halide grains with a gelatino-peptizer or with the use of certain synthetic polymers that serve as effective nucleation peptizers and then growing the silver halide grains with the use of either a gelatino-peptizer or certain synthetic polymers that serve as effective growth peptizers.
摘要翻译： 一种制备由卤化银颗粒组成的薄片状颗粒卤化银乳剂的方法，该卤化银颗粒的卤化物含量至少为50％（摩尔）溴化物，其中厚度小于0.15微米，长宽比大于8的片状颗粒占据 占总颗粒投影面积的大于50％的步骤包括用明胶胶溶剂将卤化银颗粒成核的步骤或使用某些合成聚合物作为有效的成核胶溶剂，然后使用 用作有效成长胶溶剂的明胶胶溶剂或某些合成聚合物。
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