公开(公告)号：US20110092380A1

公开(公告)日：2011-04-21

申请号：US12521765

申请日：2007-12-31

申请人： Peer Stahler ,  Markus Beier ,  Cord Stahler ,  Daniel Summerer ,  Mark Matzas ,  Sonja Vorwerk

发明人： Peer Stahler ,  Markus Beier ,  Cord Stahler ,  Daniel Summerer ,  Mark Matzas ,  Sonja Vorwerk

IPC分类号： C40B30/04 ,  C40B60/14 ,  C12Q1/68 ,  C12P19/34 ,  C40B50/00 ,  C40B30/00 ,  C40B50/06 ,  C40B40/00 ,  C40B40/06

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00702 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729

摘要： The invention relates to improved molecular-biological processing equipment and an improved method of processing biological samples. The invention combines the provision of biologically functional molecules such as nucleic acids and peptides and of derivatives or analogs of these two classes of molecules in miniaturized flow cells with the sequential addition of reagents or fluids and serves for the processing of biological samples, such as proteins, nucleic acids, biogenic small molecules such as e.g. metabolites, viruses or cells, which for this purpose are introduced into the miniaturized flow cells. The invention further relates to methods and to the use of the molecular-biological processing equipment according to the invention for the detection and/or for the isolation of nucleic acids; for sequencing; for point mutation analysis; for the analysis of genomes and/or chromosomes; for the production of synthetic nucleic acids; for the production of arrays of primers, probes and/or antisense molecules; and other analytical and synthetic methods.

摘要翻译： 本发明涉及改进的分子生物处理设备和改进的生物样品处理方法。 本发明结合了在微型流动池中提供生物功能分子例如核酸和肽以及这两类分子的衍生物或类似物，连续添加试剂或液体，并用于加工生物样品如蛋白质 ，核酸，生物来源的小分子，例如 代谢物，病毒或细胞，为此目的被引入到小型化的流动池中。 本发明还涉及根据本发明的分子生物处理设备的方法和用途，用于检测和/或分离核酸; 用于测序; 用于点突变分析; 用于分析基因组和/或染色体; 用于生产合成核酸; 用于产生引物，探针和/或反义分子的阵列; 和其他分析和合成方法。