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公开(公告)号:US09006419B2
公开(公告)日:2015-04-14
申请号:US12905777
申请日:2010-10-15
申请人: Pei-Shin Jiang , Kun-Chan Wu , Yu-Ting Su , Chia-Yun Lin , Siou-Cing Su , Yuh-Jiuan Lin
发明人: Pei-Shin Jiang , Kun-Chan Wu , Yu-Ting Su , Chia-Yun Lin , Siou-Cing Su , Yuh-Jiuan Lin
CPC分类号: C12N15/1013 , C12N15/1006
摘要: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.
摘要翻译: 提供了分离核酸的方法。 该方法包括提供含有至少一种核酸的生物样品,并在合适的条件下将生物样品与分离剂混合,以在单步骤中分离生物样品中的核酸,其中所述分离剂含有1-40重量% 的PEG和/或大于30重量%的低分子量醇,盐和洗涤剂。 分离的核酸通过核酸溶解度的变化与固体支持物结合。 另外,本发明还提供了用于分离核酸的分离剂和试剂盒。
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公开(公告)号:US20110097782A1
公开(公告)日:2011-04-28
申请号:US12905777
申请日:2010-10-15
申请人: Pei-Shin JIANG , Kun-Chan Wu , Yu-Ting Su , Chia-Yun Lin , Siou-Cing Su , Yuh-Jiuan Lin
发明人: Pei-Shin JIANG , Kun-Chan Wu , Yu-Ting Su , Chia-Yun Lin , Siou-Cing Su , Yuh-Jiuan Lin
CPC分类号: C12N15/1013 , C12N15/1006
摘要: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.
摘要翻译: 提供了分离核酸的方法。 该方法包括提供含有至少一种核酸的生物样品,并在合适的条件下将生物样品与分离剂混合,以在单步骤中分离生物样品中的核酸,其中所述分离剂含有1-40重量% 的PEG和/或大于30重量%的低分子量醇,盐和洗涤剂。 分离的核酸通过核酸溶解度的变化与固体支持物结合。 另外,本发明还提供了用于分离核酸的分离剂和试剂盒。
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