Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
    8.
    发明申请
    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways 审中-公开
    用于检测癌症信号通路中蛋白质磷酸化的试剂

    公开(公告)号:US20100151495A9

    公开(公告)日:2010-06-17

    申请号:US12074228

    申请日:2008-02-29

    CPC分类号: C07K16/44 G01N33/57426

    摘要: The invention discloses nearly 443 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Protein kinases (including Serine/Threonine dual specificity, and Tyrosine kinases), Adaptor/Scaffold proteins, Transcription factors, Phospoatases, Tumor supressors, Ubiquitin conjugating system proteins, Translation initiation complex proteins, RNA binding proteins, Apoptosis proteins, Adhesion proteins, G protein regulators/GTPase activating protein/Guanine nucleotide exchange factor proteins, and DNA binding/replication/repair proteins, as well as other protein types.

    摘要翻译: 本发明公开了在信号转导蛋白中鉴定的近443个新的磷酸化位点和人癌基因的途径,并提供磷酸化位点特异性抗体和重同位素标记肽(AQUA肽),用于选择性检测和定量这些磷酸化位点/蛋白质,如 以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点:蛋白激酶(包括丝氨酸/苏氨酸双特异性和酪氨酸激酶),适配器/支架蛋白,转录因子,磷酸酶,肿瘤抑制因子,泛素缀合系统蛋白,翻译起始复合体 蛋白质,RNA结合蛋白,细胞凋亡蛋白,粘附蛋白,G蛋白调节剂/ GTPase激活蛋白/鸟嘌呤核苷酸交换因子蛋白,以及DNA结合/复制/修复蛋白,以及其他蛋白质类型。

    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
    9.
    发明申请
    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways 审中-公开
    用于检测癌症信号通路中蛋白质磷酸化的试剂

    公开(公告)号:US20090258442A1

    公开(公告)日:2009-10-15

    申请号:US12074199

    申请日:2008-02-29

    IPC分类号: G01N33/536 C07K16/18

    摘要: The invention discloses nearly 474 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Kinase, Adaptor/Scaffold proteins, Phosphatase, G protein Regulator/Guanine Nucleotide Exchange Factors/GTPase Activating Proteins, Cytoskeleton Proteins, DNA Binding Proteins, Phospholipase, Receptor Proteins, Enzymes, DNA Repair/Replication Proteins, Adhesion Proteins, and Proteases, as well as other protein types.

    摘要翻译: 本发明公开了在信号转导蛋白中鉴定的近474个新的磷酸化位点和人癌基因的途径,并提供磷酸化位点特异性抗体和重同位素标记肽(AQUA肽),用于选择性检测和定量这些磷酸化位点/蛋白质,如 以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点:激酶,适配器/支架蛋白,磷酸酶,G蛋白调节剂/鸟嘌呤核苷酸交换因子/ GTP酶活化蛋白,细胞骨架蛋白,DNA结合蛋白,磷脂酶,受体蛋白,酶, DNA修复/复制蛋白,粘附蛋白和蛋白酶,以及其他蛋白质类型。

    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
    10.
    发明申请
    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways 审中-公开
    用于检测癌症信号通路中蛋白质磷酸化的试剂

    公开(公告)号:US20090061459A1

    公开(公告)日:2009-03-05

    申请号:US12074228

    申请日:2008-02-29

    CPC分类号: C07K16/44 G01N33/57426

    摘要: The invention discloses nearly 443 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Protein kinases (including Serine/Threonine dual specificity, and Tyrosine kinases), Adaptor/Scaffold proteins, Transcription factors, Phospoatases, Tumor supressors, Ubiquitin conjugating system proteins, Translation initiation complex proteins, RNA binding proteins, Apoptosis proteins, Adhesion proteins, G protein regulators/GTPase activating protein/Guanine nucleotide exchange factor proteins, and DNA binding/replication/repair proteins, as well as other protein types.

    摘要翻译: 本发明公开了在信号转导蛋白中鉴定的近443个新的磷酸化位点和人癌基因的途径,并提供磷酸化位点特异性抗体和重同位素标记肽(AQUA肽),用于选择性检测和定量这些磷酸化位点/蛋白质,如 以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点:蛋白激酶(包括丝氨酸/苏氨酸双特异性和酪氨酸激酶),适配器/支架蛋白,转录因子,磷酸酶,肿瘤抑制因子,泛素缀合系统蛋白,翻译起始复合体 蛋白质,RNA结合蛋白,细胞凋亡蛋白,粘附蛋白,G蛋白调节剂/ GTPase激活蛋白/鸟嘌呤核苷酸交换因子蛋白,以及DNA结合/复制/修复蛋白,以及其他蛋白质类型。