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24 条记录 (耗时 0.066 秒)

    PHOSPHO-SPECIFIC ANTIBODIES TO PI3K REGULATORY SUBUNIT AND USES THEREOF
    7.
    发明申请
    PHOSPHO-SPECIFIC ANTIBODIES TO PI3K REGULATORY SUBUNIT AND USES THEREOF 审中-公开
    磷酸特异性抗PI3K调节子及其用途

    公开(公告)号：US20080014598A1

    公开(公告)日：2008-01-17

    申请号：US11744753

    申请日：2007-05-04

    申请人： Thorsten Wiederhold ,  Valerie Goss ,  Albrecht Moritz ,  Klarisa Rikova ,  Ting-Lei Gu ,  Peter Hornbeck

    发明人： Thorsten Wiederhold ,  Valerie Goss ,  Albrecht Moritz ,  Klarisa Rikova ,  Ting-Lei Gu ,  Peter Hornbeck

    IPC分类号： G01N33/573 ,  C07K16/18 ,  C12N5/06

    CPC分类号： C07K16/40 ,  C12Q1/485 ,  G01N33/6842 ,  G01N2333/91215

    摘要： The invention discloses ten newly discovered PI3K regulatory subunit phosphorylation sites, tyrosines 467, 452, 463, and 470 in PI3KR1 (PI3Kp85 alpha), tyrosines 464, 460, and 467 in PI3KR2 (PI3Kp85 beta), and tyrosines 199, 184, and 202 in PI3KR3 (PI3Kp55 gamma), and provides reagents, including polyclonal and monoclonal antibodies, that selectively bind to PI3K when phosphorylated at one of the disclosed sites. Also provided are assays utilizing this reagent, including methods for determining the phosphorylation of PI3K in a biological sample, selecting a patient suitable for PI3K inhibitor therapy, profiling PI3K activation in a test tissue, and identifying a compound that modulates phosphorylation of PI3K in a test tissue, by using a detectable reagent, such as the disclosed antibody, that binds to PI3K only when phosphorylated at a disclosed site. The sample or test tissue may be taken from a subject suspected of having cancer, such as lymphoma, glioma, and colon cancer, involving altered PI3K signaling.

    摘要翻译： 本发明公开了PI3KR1（PI3Kp85α），PI3KR2（PI3Kp85β）中酪氨酸464,460和467和酪氨酸199,184和202中的10个新发现的PI3K调节亚基磷酸化位点，酪氨酸467,452,463和470 在PI3KR3（PI3Kp55γ）中，并且提供试剂，包括多克隆和单克隆抗体，当在所公开的位点之一磷酸化时选择性地结合PI3K。 还提供了使用该试剂的测定法，包括用于测定生物样品中PI3K的磷酸化的方法，选择适合于PI3K抑制剂治疗的患者，在测试组织中分析PI3K活化，以及鉴定在测试中调节PI3K磷酸化的化合物 组织，通过使用仅在所公开的位点磷酸化时与PI3K结合的可检测试剂，例如所公开的抗体。 样品或测试组织可以取自怀疑患有癌症的受试者，例如淋巴瘤，神经胶质瘤和结肠癌，涉及改变的PI3K信号传导。

    Reagens for the detection of protein acetylation signaling pathways
    10.
    发明申请
    Reagens for the detection of protein acetylation signaling pathways 审中-公开
    用于检测蛋白质乙酰化信号通路的试剂

    公开(公告)号：US20090325312A1

    公开(公告)日：2009-12-31

    申请号：US12227320

    申请日：2007-05-11

    申请人： Ailan Guo ,  Ting-Lei Gu ,  Peter Hornbeck ,  Jeffrey Mitchell ,  Yu Li

    发明人： Ailan Guo ,  Ting-Lei Gu ,  Peter Hornbeck ,  Jeffrey Mitchell ,  Yu Li

    IPC分类号： G01N33/566 ,  C07K16/18

    CPC分类号： G01N33/6842 ,  C07K16/44

    摘要： The invention discloses 426 novel acetylation sites identified in signal transduction proteins and pathways underlying human protein acetylation signaling pathways, and provides acetylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these acetylated sites/proteins, as well as methods of using the reagents for such purpose. Among the acetylation sites identified are sites occurring in the following protein types: Methyltransferases, Transcription factors, Transcription coactivators, Translation initiation complex proteins, Oxireductases, Protein kinases, RNA binding proteins, Secreted proteins, Transferases, Transporter proteins, Ubiquitin conjugating system proteins, Motor proteins, Phosphotases, Proteases, Phospholipases, Receptor proteins and Vesicle proteins.

    摘要翻译： 本发明公开了在信号转导蛋白中鉴定的426个新的乙酰化位点和人蛋白质乙酰化信号通路的途径，并提供乙酰化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些乙酰化位点/蛋白质 ，以及使用试剂用于此目的的方法。 确定的乙酰化位点之间的位点是发生在以下蛋白质类型中的位点：甲基转移酶，转录因子，转录共激活因子，翻译起始复合蛋白​​，氧化还原酶，蛋白激酶，RNA结合蛋白，分泌蛋白，转移酶，转运蛋白，泛素缀合系统蛋白，马达 蛋白质，磷酸酶，蛋白酶，磷脂酶，受体蛋白和囊泡蛋白。