公开(公告)号：US20100292154A1

公开(公告)日：2010-11-18

申请号：US11917661

申请日：2006-06-16

申请人： Peter J. Millett ,  David A. Sarracino ,  Bryan Krastins ,  Reuben Gobezie

发明人： Peter J. Millett ,  David A. Sarracino ,  Bryan Krastins ,  Reuben Gobezie

IPC分类号： A61K38/00 ,  C07H21/04 ,  C40B40/10 ,  G01N33/566 ,  C12Q1/68 ,  G01N33/573 ,  G01N33/53 ,  C40B30/04 ,  A61P19/02

CPC分类号： G01N33/6893 ,  G01N2800/105

摘要： The present invention relates to the identification and use of protein expression profiles with clinical relevance to osteoarthritis (OA). In particular, the invention provides the identity of marker proteins whose expression is correlated with OA and OA progression. Methods and kits are described for using these protein expression profiles in the study and/or diagnosis of OA, in the determination of the degree of advancement of OA, and in the selection and/or monitoring of treatment regimens. The invention also relates to the screening of drugs that modulate expression of these proteins or nucleic acid molecules encoding these proteins, in particular for the development of disease-modifying OA agents.

摘要翻译： 本发明涉及与骨关节炎（OA）临床相关的蛋白质表达谱的鉴定和应用。 特别地，本发明提供了表达与OA和OA进展相关的标记蛋白的身份。 描述了在研究和/或诊断OA中使用这些蛋白质表达谱的方法和试剂盒，以确定OA的进展程度，以及在治疗方案的选择和/或监测中。 本发明还涉及调节这些蛋白质或编码这些蛋白质的核酸分子的表达的药物的筛选，特别是用于发展改变疾病的OA剂。

公开(公告)号：US20120263724A1

公开(公告)日：2012-10-18

申请号：US11919021

申请日：2006-04-20

申请人： Manohar John ,  Indira T. Kudva ,  Stephen B. Calderwood ,  Bryan Krastins ,  David A. Sarracino

发明人： Manohar John ,  Indira T. Kudva ,  Stephen B. Calderwood ,  Bryan Krastins ,  David A. Sarracino

IPC分类号： C07K14/245 ,  C12N15/63 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  C12P21/00 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/566 ,  A61K39/108 ,  A61P37/04 ,  A61P31/04 ,  C12N15/31 ,  C07K16/12 ,  A61K39/40 ,  C40B20/08

CPC分类号： C07K14/245 ,  A61K39/00

摘要： Immunogenic Escherichia coli O157:H7 (0157) proteins expressed during infection of a mammal, are described. In particular, 0157 proteins expressed specifically in vivo during human and cattle infection were identified. These proteins, mapped to the backbone, O-islands (OIs) and pO157. Because these-proteins are expressed during infection, and might help pathogens adapt to and counter hostile in vivo environments, those proteins identified in this study are useful as targets for drug and vaccine development. Also, such proteins are useful as markers of 0157 infection in stool specimens. Also described are methods of identifying immunogenic proteins.

摘要翻译： 描述了在哺乳动物感染期间表达的免疫原性大肠杆菌O157：H7（0157）蛋白质。 特别地，鉴定了在人和牛感染期间在体内特异性表达的0157个蛋白质。 这些蛋白质，映射到骨架，O岛（OIs）和pO157。 因为这些蛋白质在感染期间表达，并且可能有助于病原体适应和反对敌对的体内环境，本研究中鉴定的蛋白质可用作药物和疫苗开发的靶标。 此外，这些蛋白质可用作粪便标本中0157感染的标志物。 还描述了鉴定免疫原性蛋白质的方法。

公开(公告)号：US20100216250A1

公开(公告)日：2010-08-26

申请号：US12710062

申请日：2010-02-22

申请人： Mary Frances LOPEZ ,  David A. Sarracino ,  Bryan E. Krastins ,  Taha Rezai ,  Amol Prakash ,  Michael J. Athanas

发明人： Mary Frances LOPEZ ,  David A. Sarracino ,  Bryan E. Krastins ,  Taha Rezai ,  Amol Prakash ,  Michael J. Athanas

IPC分类号： G01N33/50

CPC分类号： G01N33/689 ,  G01N33/6848 ,  G01N2333/775 ,  G01N2333/805 ,  G01N2800/387

摘要： The present invention relates to biomarkers in maternal serum samples and methods for predicting whether a fetus has Trisomy 21 during the first trimester of pregnancy based on the differential expression of a combination, or subcombination, of the same.

摘要翻译： 本发明涉及母体血清样品中的生物标志物和基于组合的差异表达或其亚组合来预测胎儿在妊娠前三个月是否具有三体性21的方法。

公开(公告)号：US20110151568A1

公开(公告)日：2011-06-23

申请号：US12644284

申请日：2009-12-22

申请人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

发明人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

IPC分类号： G01N33/00

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。

公开(公告)号：US08383417B2

公开(公告)日：2013-02-26

申请号：US12644284

申请日：2009-12-22

申请人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

发明人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

IPC分类号： G01N24/00 ,  G01N33/00

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。