公开(公告)号：US09587036B2

公开(公告)日：2017-03-07

申请号：US13638223

申请日：2011-04-01

申请人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carola Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

发明人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carola Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

IPC分类号： C07K16/46 ,  A61K39/395 ,  C07K16/28 ,  C07K16/30

CPC分类号： C07K16/468 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20130129730A1

公开(公告)日：2013-05-23

申请号：US13638223

申请日：2011-04-01

申请人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carole Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

发明人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carole Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

IPC分类号： C07K16/46

CPC分类号： C07K16/468 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20110293619A1

公开(公告)日：2011-12-01

申请号：US13122245

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lütterbuse ,  Patrick Hoffmann ,  Doris Rau ,  Susanne Mangold ,  Matthias Klinger ,  Evelyne aschaller ,  Susanne Hausmann ,  Petra Fluhr ,  Carola Steiger

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lütterbuse ,  Patrick Hoffmann ,  Doris Rau ,  Susanne Mangold ,  Matthias Klinger ,  Evelyne aschaller ,  Susanne Hausmann ,  Petra Fluhr ,  Carola Steiger

IPC分类号： A61K39/395 ,  C12N5/10 ,  C12N1/00 ,  A61P35/00 ,  C12N15/63 ,  C07K16/18 ,  C07H21/04 ,  C12P21/00 ,  C12N1/21

CPC分类号： C07K16/3069 ,  A61K2039/505 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain anti-body molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20100183615A1

公开(公告)日：2010-07-22

申请号：US12594729

申请日：2008-04-03

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07H21/04 ,  A61P35/00 ,  A61P37/00 ,  C12N15/74 ,  C12N5/10

CPC分类号： C07K16/2809 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/40 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2319/32 ,  C07K2319/43

摘要： The present invention relates to a polypeptide comprising a first human binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 (epsilon) chain and a second binding domain capable of binding to EGFR, Her2/neu or IgE of a human and/or a non-chimpanzee primate as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acids encoding for the polypeptide, to vectors comprising the same and to host cells comprising the vector. In another aspect, the invention provides for a pharmaceutical composition comprising the mentioned polypeptide and medical uses of the polypeptide.

摘要翻译： 本发明涉及包含能够结合人和非黑猩猩灵长类CD3（ε）链的表位的第一人结合结构域和能够结合人的EGFR，Her2 / neu或IgE的第二结合结构域的多肽 和/或非黑猩猩灵长类动物以及生产所述多肽的方法。 本发明还涉及编码多肽的核酸，包含其的载体和包含载体的宿主细胞。 另一方面，本发明提供包含所述多肽的药物组合物和多肽的医学用途。

公开(公告)号：US20100150918A1

公开(公告)日：2010-06-17

申请号：US12594713

申请日：2008-04-03

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： A61K39/395 ,  C07K16/28 ,  C07H21/04 ,  C12N15/63 ,  A61P35/00 ,  A61P37/00

CPC分类号： C07K16/082 ,  A61K2039/505 ,  C07K14/7051 ,  C07K16/109 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/2863 ,  C07K16/2878 ,  C07K16/2884 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2319/30 ,  C07K2319/40

摘要： The present invention relates to a polypeptide comprising a human binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 (epsilon) chain as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acids encoding for the polypeptide, to vectors comprising the same and to host cells comprising the vector. In another aspect, the invention provides for a pharmaceutical composition comprising the mentioned polypeptide and medical uses of the polypeptide. In a further aspect the invention provides a method for the identification of polypeptides comprising a cross-species specific binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3ε (CD3 epsilon).

摘要翻译： 本发明涉及包含能够结合人和非黑猩猩灵长类CD3（ε）链的表位的人结合结构域的多肽以及生产所述多肽的方法。 本发明还涉及编码多肽的核酸，包含其的载体和包含载体的宿主细胞。 另一方面，本发明提供包含所述多肽的药物组合物和多肽的医学用途。 在另一方面，本发明提供了用于鉴定多肽的方法，所述多肽包含能够结合人和非黑猩猩灵长类CD3抗原决定簇的物种间特异性结合结构域; （CD3ε）。

公开(公告)号：US20120244162A1

公开(公告)日：2012-09-27

申请号：US13408363

申请日：2012-02-29

申请人： Peter KUFER ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter KUFER ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： C07K16/46 ,  A61K39/395 ,  A61P35/00 ,  C12N1/19 ,  C12N15/63 ,  C12P21/00 ,  C12N1/21 ,  C12N5/10 ,  C12N15/13 ,  A61P37/00

CPC分类号： C07K16/2809 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/40 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2319/32 ,  C07K2319/43

摘要： The present invention relates to a polypeptide comprising a first human binding domain capbable of binding to an epitope of human and non-chimpanzee CD3ε (epsilon) chain and a second binding domain capable of binding to EGFR, Her2/neu or IgE of a human and/or a non-chimpanzee primate as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acid sequences encoding the polypeptide, to vectors comprising the nucleic acid sequences and to host cells comprising the nucleic acid sequences or vectors containing the nucleic acid sequences. In another aspect, the invention provides for a pharmaceutical composition comprising the polypeptide and methods of medical treatment or use of the polypeptide.

摘要翻译： 本发明涉及包含可与人和非黑猩猩CD3抗原表位结合的第一人结合结构域的多肽; （ε）链和能够结合人和/或非黑猩猩灵长类动物的EGFR，Her2 / neu或IgE的第二结合结构域以及生产所述多肽的方法。 本发明还涉及编码该多肽的核酸序列，包含核酸序列的载体和包含核酸序列或含有该核酸序列的载体的宿主细胞。 另一方面，本发明提供了包含该多肽的药物组合物和该多肽的医学治疗或使用方法。

公开(公告)号：US20120034228A1

公开(公告)日：2012-02-09

申请号：US13122242

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Doris Rau ,  Matthias Klinger ,  Susanne Mangold ,  Claudia Blümel

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Doris Rau ,  Matthias Klinger ,  Susanne Mangold ,  Claudia Blümel

IPC分类号： A61K39/395 ,  C12N15/62 ,  C12N15/63 ,  C12P21/02 ,  A61P35/02 ,  C12N1/19 ,  C12N5/10 ,  C12N1/15 ,  A61P35/00 ,  C07K16/46 ,  C12N1/21

CPC分类号： C07K16/2809 ,  A61K2039/505 ,  C07K16/22 ,  C07K16/2803 ,  C07K16/2851 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3069 ,  C07K16/40 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to an antigen selected from the group consisting of Prostate Stem Cell Antigen (PSCA), B-Lymphocyte antigen CD19 (CD19), hepatocyte growth factor receptor (C-MET), Endosialin, the EGF-like domain 1 of EpCAM, encoded by exon 2, Fibroblast activation protein alpha (FAP alpha) and Insulin-like growth factor I receptor (IGF-IR or IGF-1R). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合选自前列腺干细胞抗原（PSCA），B-淋巴细胞抗原CD19（CD19），肝细胞生长因子受体（C- MET），内皮唾液酸蛋白，由外显子2编码的EpCAM的EGF样结构域1，成纤维细胞激活蛋白α（FAPα）和胰岛素样生长因子I受体（IGF-1R或IGF-1R）。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US10981998B2

公开(公告)日：2021-04-20

申请号：US13122280

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffman ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffman ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： C07K16/28

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain consisting of one antibody variable domain capable of binding to an epitope of the human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to an epitope of a human and a non-chimpanzee primate tumor target antigen. The invention further relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3ϵ (epsilon) chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain consisting of one antibody variable domain capable of binding to an epitope of a human and a non-chimpanzee primate tumor target antigen. The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

公开(公告)号：US20090226432A1

公开(公告)日：2009-09-10

申请号：US12158611

申请日：2006-12-21

申请人： Ralf Lutterbüse ,  Petra Mayer ,  Evelyne Schaller ,  Doris Rau ,  Susanne Mangold ,  Peter Kufer ,  Alexander Murr ,  Tobias Raum ,  Monika Wissinger

发明人： Ralf Lutterbüse ,  Petra Mayer ,  Evelyne Schaller ,  Doris Rau ,  Susanne Mangold ,  Peter Kufer ,  Alexander Murr ,  Tobias Raum ,  Monika Wissinger

IPC分类号： A61K39/395 ,  A61K31/7088 ,  C12P21/04

CPC分类号： C07K16/468 ,  A61K39/39558 ,  A61K45/06 ,  C07K16/2809 ,  C07K16/3007 ,  C07K16/46 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2319/00

摘要： The present invention relates to pharmaceutical compositions for the treatment of an epithelial tumor in a human, said pharmaceutical composition comprising a bispecific single chain antibody which has a first binding domain specifically binding to human CD3, and a second binding domain specifically binding to human CEA, wherein said second binding domain comprises at least a part of the CDR-H3 or the complete CDR-H3 of murine monoclonal antibody A5B7. Furthermore, processes for the production of said pharmaceutical compositions as well as medical/pharmaceutical uses for the specific bispecific single chain antibody molecules bearing specificities for the human CD3 antigen and the human CEA antigen are disclosed.

摘要翻译： 本发明涉及用于治疗人类上皮肿瘤的药物组合物，所述药物组合物包含具有特异性结合人CD3的第一结合结构域的双特异性单链抗体和特异性结合人CEA的第二结合结构域， 其中所述第二结合结构域包含鼠单克隆抗体A5B7的CDR-H3或完整CDR-H3的至少一部分。 此外，公开了用于生产所述药物组合物的方法以及对于人CD3抗原和人CEA抗原具有特异性的特异性双特异性单链抗体分子的医学/药物用途。

公开(公告)号：US08394926B2

公开(公告)日：2013-03-12

申请号：US12158611

申请日：2006-12-21

申请人： Ralf Lutterbüse ,  Petra Mayer ,  Evelyne Schaller ,  Doris Rau ,  Susanne Mangold ,  Peter Kufer ,  Alexander Murr ,  Tobias Raum ,  Monika Wissinger

发明人： Ralf Lutterbüse ,  Petra Mayer ,  Evelyne Schaller ,  Doris Rau ,  Susanne Mangold ,  Peter Kufer ,  Alexander Murr ,  Tobias Raum ,  Monika Wissinger

IPC分类号： C12P21/08

CPC分类号： C07K16/468 ,  A61K39/39558 ,  A61K45/06 ,  C07K16/2809 ,  C07K16/3007 ,  C07K16/46 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2319/00

摘要： The present disclosure relates to a bispecific single chain antibody which has a first binding domain specifically binding to human CD3, and a second binding domain specifically binding to human CEA, where the second binding domain comprises at least a part of the CDR-H3 or the complete CDR-H3 of murine monoclonal antibody A5B7, a pharmaceutical composition comprising the bispecific single chain antibody, and methods for the treatment of an epithelial tumor in a human with the pharmaceutical compositions containing the bispecific single chain antibody. Furthermore, processes for the production of the pharmaceutical compositions as well as medical/pharmaceutical uses for the specific bispecific single chain antibody molecules bearing specificities for the human CD3 antigen and the human CEA antigen are disclosed.

摘要翻译： 本公开内容涉及具有特异性结合人CD3的第一结合结构域和特异性结合人CEA的第二结合结构域的双特异性单链抗体，其中第二结合结构域包含至少一部分CDR-H3或 含有双特异性单链抗体的单克隆抗体A5B7的完整CDR-H3，包含双特异性单链抗体的药物组合物，以及用含有双特异性单链抗体的药物组合物治疗人的上皮肿瘤的方法。 此外，公开了用于生产药物组合物的方法以及具有对人CD3抗原和人CEA抗原具有特异性的特异性双特异性单链抗体分子的医学/药物用途。