公开(公告)号：US09587036B2

公开(公告)日：2017-03-07

申请号：US13638223

申请日：2011-04-01

申请人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carola Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

发明人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carola Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

IPC分类号： C07K16/46 ,  A61K39/395 ,  C07K16/28 ,  C07K16/30

CPC分类号： C07K16/468 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20130129730A1

公开(公告)日：2013-05-23

申请号：US13638223

申请日：2011-04-01

申请人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carole Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

发明人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carole Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

IPC分类号： C07K16/46

CPC分类号： C07K16/468 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20110293619A1

公开(公告)日：2011-12-01

申请号：US13122245

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lütterbuse ,  Patrick Hoffmann ,  Doris Rau ,  Susanne Mangold ,  Matthias Klinger ,  Evelyne aschaller ,  Susanne Hausmann ,  Petra Fluhr ,  Carola Steiger

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lütterbuse ,  Patrick Hoffmann ,  Doris Rau ,  Susanne Mangold ,  Matthias Klinger ,  Evelyne aschaller ,  Susanne Hausmann ,  Petra Fluhr ,  Carola Steiger

IPC分类号： A61K39/395 ,  C12N5/10 ,  C12N1/00 ,  A61P35/00 ,  C12N15/63 ,  C07K16/18 ,  C07H21/04 ,  C12P21/00 ,  C12N1/21

CPC分类号： C07K16/3069 ,  A61K2039/505 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain anti-body molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20100183615A1

公开(公告)日：2010-07-22

申请号：US12594729

申请日：2008-04-03

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07H21/04 ,  A61P35/00 ,  A61P37/00 ,  C12N15/74 ,  C12N5/10

CPC分类号： C07K16/2809 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/40 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2319/32 ,  C07K2319/43

摘要： The present invention relates to a polypeptide comprising a first human binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 (epsilon) chain and a second binding domain capable of binding to EGFR, Her2/neu or IgE of a human and/or a non-chimpanzee primate as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acids encoding for the polypeptide, to vectors comprising the same and to host cells comprising the vector. In another aspect, the invention provides for a pharmaceutical composition comprising the mentioned polypeptide and medical uses of the polypeptide.

摘要翻译： 本发明涉及包含能够结合人和非黑猩猩灵长类CD3（ε）链的表位的第一人结合结构域和能够结合人的EGFR，Her2 / neu或IgE的第二结合结构域的多肽 和/或非黑猩猩灵长类动物以及生产所述多肽的方法。 本发明还涉及编码多肽的核酸，包含其的载体和包含载体的宿主细胞。 另一方面，本发明提供包含所述多肽的药物组合物和多肽的医学用途。

公开(公告)号：US20100150918A1

公开(公告)日：2010-06-17

申请号：US12594713

申请日：2008-04-03

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： A61K39/395 ,  C07K16/28 ,  C07H21/04 ,  C12N15/63 ,  A61P35/00 ,  A61P37/00

CPC分类号： C07K16/082 ,  A61K2039/505 ,  C07K14/7051 ,  C07K16/109 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/2863 ,  C07K16/2878 ,  C07K16/2884 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2319/30 ,  C07K2319/40

摘要： The present invention relates to a polypeptide comprising a human binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 (epsilon) chain as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acids encoding for the polypeptide, to vectors comprising the same and to host cells comprising the vector. In another aspect, the invention provides for a pharmaceutical composition comprising the mentioned polypeptide and medical uses of the polypeptide. In a further aspect the invention provides a method for the identification of polypeptides comprising a cross-species specific binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3ε (CD3 epsilon).

摘要翻译： 本发明涉及包含能够结合人和非黑猩猩灵长类CD3（ε）链的表位的人结合结构域的多肽以及生产所述多肽的方法。 本发明还涉及编码多肽的核酸，包含其的载体和包含载体的宿主细胞。 另一方面，本发明提供包含所述多肽的药物组合物和多肽的医学用途。 在另一方面，本发明提供了用于鉴定多肽的方法，所述多肽包含能够结合人和非黑猩猩灵长类CD3抗原决定簇的物种间特异性结合结构域; （CD3ε）。

公开(公告)号：US20120244162A1

公开(公告)日：2012-09-27

申请号：US13408363

申请日：2012-02-29

申请人： Peter KUFER ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter KUFER ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： C07K16/46 ,  A61K39/395 ,  A61P35/00 ,  C12N1/19 ,  C12N15/63 ,  C12P21/00 ,  C12N1/21 ,  C12N5/10 ,  C12N15/13 ,  A61P37/00

CPC分类号： C07K16/2809 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/40 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2319/32 ,  C07K2319/43

摘要： The present invention relates to a polypeptide comprising a first human binding domain capbable of binding to an epitope of human and non-chimpanzee CD3ε (epsilon) chain and a second binding domain capable of binding to EGFR, Her2/neu or IgE of a human and/or a non-chimpanzee primate as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acid sequences encoding the polypeptide, to vectors comprising the nucleic acid sequences and to host cells comprising the nucleic acid sequences or vectors containing the nucleic acid sequences. In another aspect, the invention provides for a pharmaceutical composition comprising the polypeptide and methods of medical treatment or use of the polypeptide.

摘要翻译： 本发明涉及包含可与人和非黑猩猩CD3抗原表位结合的第一人结合结构域的多肽; （ε）链和能够结合人和/或非黑猩猩灵长类动物的EGFR，Her2 / neu或IgE的第二结合结构域以及生产所述多肽的方法。 本发明还涉及编码该多肽的核酸序列，包含核酸序列的载体和包含核酸序列或含有该核酸序列的载体的宿主细胞。 另一方面，本发明提供了包含该多肽的药物组合物和该多肽的医学治疗或使用方法。

公开(公告)号：US20120034228A1

公开(公告)日：2012-02-09

申请号：US13122242

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Doris Rau ,  Matthias Klinger ,  Susanne Mangold ,  Claudia Blümel

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Doris Rau ,  Matthias Klinger ,  Susanne Mangold ,  Claudia Blümel

IPC分类号： A61K39/395 ,  C12N15/62 ,  C12N15/63 ,  C12P21/02 ,  A61P35/02 ,  C12N1/19 ,  C12N5/10 ,  C12N1/15 ,  A61P35/00 ,  C07K16/46 ,  C12N1/21

CPC分类号： C07K16/2809 ,  A61K2039/505 ,  C07K16/22 ,  C07K16/2803 ,  C07K16/2851 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3069 ,  C07K16/40 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to an antigen selected from the group consisting of Prostate Stem Cell Antigen (PSCA), B-Lymphocyte antigen CD19 (CD19), hepatocyte growth factor receptor (C-MET), Endosialin, the EGF-like domain 1 of EpCAM, encoded by exon 2, Fibroblast activation protein alpha (FAP alpha) and Insulin-like growth factor I receptor (IGF-IR or IGF-1R). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合选自前列腺干细胞抗原（PSCA），B-淋巴细胞抗原CD19（CD19），肝细胞生长因子受体（C- MET），内皮唾液酸蛋白，由外显子2编码的EpCAM的EGF样结构域1，成纤维细胞激活蛋白α（FAPα）和胰岛素样生长因子I受体（IGF-1R或IGF-1R）。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US10981998B2

公开(公告)日：2021-04-20

申请号：US13122280

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffman ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffman ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： C07K16/28

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain consisting of one antibody variable domain capable of binding to an epitope of the human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to an epitope of a human and a non-chimpanzee primate tumor target antigen. The invention further relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3ϵ (epsilon) chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain consisting of one antibody variable domain capable of binding to an epitope of a human and a non-chimpanzee primate tumor target antigen. The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

公开(公告)号：US08784821B1

公开(公告)日：2014-07-22

申请号：US10554731

申请日：2004-05-26

申请人： Peter Kufer ,  Tobias Raum ,  Meera Berry ,  Roman Kischel ,  Susanne Mangold ,  Eva Krinner ,  Birgit Kohleisen ,  Steven Zeman ,  Christian Itin ,  Patrick Bäuerle

发明人： Peter Kufer ,  Tobias Raum ,  Meera Berry ,  Roman Kischel ,  Susanne Mangold ,  Eva Krinner ,  Birgit Kohleisen ,  Steven Zeman ,  Christian Itin ,  Patrick Bäuerle

IPC分类号： A61K38/00

CPC分类号： C07K16/24 ,  A61K2039/505 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2896 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention provides a method for the preparation of a human binding molecule, fragment or derivative thereof which specifically binds to the human CD3 complex. Furthermore, the invention provides a human binding molecules specifically binding to the human CD3 complex and means comprising said human binding molecules.

摘要翻译： 本发明提供了制备与人CD3复合物特异性结合的人结合分子，其片段或衍生物的方法。 此外，本发明提供了特异性结合人CD3复合物的人结合分子和包含所述人结合分子的手段。

公开(公告)号：US08236308B2

公开(公告)日：2012-08-07

申请号：US12083351

申请日：2006-10-10

申请人： Roman Kischel ,  Tobias Raum ,  Bernd Schlereth ,  Doris Rau ,  Ronny Cierpka ,  Peter Kufer

发明人： Roman Kischel ,  Tobias Raum ,  Bernd Schlereth ,  Doris Rau ,  Ronny Cierpka ,  Peter Kufer

IPC分类号： A61K39/395

CPC分类号： A61K39/39558 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/40 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/622

摘要： The present invention relates to uses of bispecific antibodies exhibiting cross-species specificity for evaluating the in vivo safety and/or activity and/or pharmacokinetic profile of the same in non-human species and humans. The present invention moreover relates to methods for evaluating the in vivo safety and/or activity and/or pharmacokinetic profile of said bispecific anti-bodies exhibiting cross-species specificity. The present invention also relates to methods of measuring the biological activity and/or efficacy of such bispecific antibodies exhibiting cross-species specificity. In addition, the present invention relates to pharmaceutical compositions comprising bispecific single chain antibodies exhibiting cross-species specificity and to methods for the preparation of pharmaceutical compositions comprising said bispecific single chain antibodies exhibiting cross-species specificity for the treatment of diseases.

摘要翻译： 本发明涉及显示跨物种特异性的双特异性抗体用于评估其在非人物种和人中的体内安全性和/或其活性和/或药代动力学特征的用途。 本发明还涉及用于评估展示跨物种特异性的所述双特异性抗体的体内安全性和/或活性和/或药代动力学特征的方法。 本发明还涉及测量显示跨物种特异性的这种双特异性抗体的生物活性和/或功效的方法。 此外，本发明涉及包含显示跨物种特异性的双特异性单链抗体的药物组合物和用于制备药物组合物的方法，所述药物组合物包含显示针对疾病治疗疾病的跨物种特异性的所述双特异性单链抗体。