公开(公告)号：US20050019956A1

公开(公告)日：2005-01-27

申请号：US10616251

申请日：2003-07-08

申请人： Peter Martin ,  Keiki-Pua Dancil ,  Arthur Morsell ,  Hus Tigli

发明人： Peter Martin ,  Keiki-Pua Dancil ,  Arthur Morsell ,  Hus Tigli

IPC分类号： B01L3/00 ,  G01N21/84 ,  G01N33/552 ,  G01N35/00

CPC分类号： B01L3/502753 ,  B01L3/5023 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0655 ,  G01N21/77 ,  G01N33/552 ,  G01N2021/752 ,  G01N2021/755 ,  G01N2021/757 ,  G01N2021/758 ,  G01N2021/7779 ,  G01N2021/7796 ,  G01N2035/0097 ,  Y10S435/808

摘要： Methods and devices for the measurement of molecular binding interactions. Preferred embodiments provide real-time measurements of kinetic binding and disassociation of molecules including binding and disassociation of protein molecules with other protein molecules and with other molecules. In preferred embodiments ligands are immobilized within pores of a porous silicon interaction region produced in a silicon substrate, after which analytes suspended in a fluid are flowed over the porous silicon region. Binding reactions occur when analyte molecules diffuse closely enough to the ligands to become bound. Preferably the binding and subsequent disassociation reactions are observed utilizing a white light source and thin film interference techniques with spectrometers arranged to detect changes in indices of refraction in the region where the binding and disassociation reactions occur. In preferred embodiments both ligands and analytes are delivered by computer controlled robotic fluid flow control techniques to the porous silicon interaction regions through microfluidic flow channels.

摘要翻译： 用于测量分子结合相互作用的方法和装置。 优选的实施方案提供动力学结合和分离的实时测量，包括蛋白质分子与其它蛋白质分子和其它分子的结合和解离。 在优选的实施方案中，将配体固定在硅衬底中产生的多孔硅相互作用区域的孔内，之后悬浮在流体中的分析物流过多孔硅区域。 结合反应发生在分析物分子向配体扩散得足够接近以成为结合物时。 优选地，使用白光源和薄膜干涉技术观察结合和随后的分离反应，其中光谱仪布置成检测发生结合和解离反应的区域中的折射指数的变化。 在优选实施方案中，配体和分析物通过计算机控制的机器人流体控制技术通过微流体流动通道传递到多孔硅相互作用区域。