公开(公告)号：US10190131B2

公开(公告)日：2019-01-29

申请号：US12280090

申请日：2007-02-21

申请人： Petra Cirpus ,  Jörg Bauer ,  Xiao Qiu ,  Guohai Wu ,  Bifang Cheng ,  Martin Truksa ,  Tom Wetjen

发明人： Petra Cirpus ,  Jörg Bauer ,  Xiao Qiu ,  Guohai Wu ,  Bifang Cheng ,  Martin Truksa ,  Tom Wetjen

IPC分类号： A61K31/20 ,  C12N15/82 ,  C12N9/02 ,  C12N9/10 ,  C12N15/52 ,  C12P7/64

摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

公开(公告)号：US20100227924A1

公开(公告)日：2010-09-09

申请号：US12280090

申请日：2007-02-21

申请人： Petra Cirpus ,  Jörg Bauer ,  Xiao Qiu ,  Guohai Wu ,  Bifang Cheng ,  Martin Truksa ,  Tom Wetjen

发明人： Petra Cirpus ,  Jörg Bauer ,  Xiao Qiu ,  Guohai Wu ,  Bifang Cheng ,  Martin Truksa ,  Tom Wetjen

IPC分类号： A61K31/20 ,  C12N15/82 ,  A01H5/00 ,  C07H21/04 ,  A23D9/00

CPC分类号： C12N15/8247 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/6427 ,  C12Y114/19

摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

摘要翻译： 本发明涉及在转基因植物中生产二十烷酸，二十二烷酸和/或二十二酸的方法。 根据所述方法，给植物提供至少一个编码具有＆Dgr .6去饱和酶活性的多肽的核酸序列，至少一个编码具有＆Dgr .6延长酶活性的多肽的核酸序列，至少一个核酸 编码具有＆Dgr。5去饱和酶活性的多肽的酸序列和编码具有＆Dgr.5延伸酶活性的多肽的至少一个核酸序列，编码具有＆Dgr。5延伸酶活性的多肽的核酸序列被修饰 相对于来自该序列的生物体中的核酸序列，使得其适于至少一种类型的植物中的密码子使用。 为了生产二十二碳六烯酸，还将至少一种编码具有＆Dgr。4去饱和酶活性的多肽的核酸序列引入植物中。