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公开(公告)号：US10190131B2
公开(公告)日：2019-01-29
申请号：US12280090
申请日：2007-02-21
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
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公开(公告)号：US20100227924A1
公开(公告)日：2010-09-09
申请号：US12280090
申请日：2007-02-21
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
CPC分类号： C12N15/8247 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , C12Y114/19
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
摘要翻译： 本发明涉及在转基因植物中生产二十烷酸，二十二烷酸和/或二十二酸的方法。 根据所述方法，给植物提供至少一个编码具有＆Dgr .6去饱和酶活性的多肽的核酸序列，至少一个编码具有＆Dgr .6延长酶活性的多肽的核酸序列，至少一个核酸 编码具有＆Dgr。5去饱和酶活性的多肽的酸序列和编码具有＆Dgr.5延伸酶活性的多肽的至少一个核酸序列，编码具有＆Dgr。5延伸酶活性的多肽的核酸序列被修饰 相对于来自该序列的生物体中的核酸序列，使得其适于至少一种类型的植物中的密码子使用。 为了生产二十二碳六烯酸，还将至少一种编码具有＆Dgr。4去饱和酶活性的多肽的核酸序列引入植物中。
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3.发明授权Method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants 有权在有用的转基因植物中生产花生四烯酸和/或二十碳五烯酸的方法公开(公告)号：US08134046B2
公开(公告)日：2012-03-13
申请号：US11990244
申请日：2006-08-01
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A61K8/361 , A61Q19/00 , C12N9/0083 , C12N9/1029
摘要： The present invention relates to a process for producing arachidonic acid and/or eicosapentaenoic acid in transgenic useful plants by introducing into the plant nucleic acids coding for polypeptides having Δ6-desaturase, Δ6-elongase or Δ5-desaturase activity. Furthermore, a gene coding for an ω3-desaturase is advantageously expressed in said useful plants. In another advantageous embodiment of the process, further nucleic acid sequences coding for polypeptides of fatty acid or lipid metabolism biosynthesis may be expressed in the plants. Particularly advantageous nucleic acid sequences here are those coding for a Δ8-desaturase, Δ12-desaturase, Δ15-desaturase, Δ4-desaturase, Δ9-elongase and/or Δ5-elongase activity.The invention further relates to the use of the oils, lipids and/or fatty acids produced in the process according to the invention in feedstuffs or foodstuffs, cosmetics or pharmaceuticals.
摘要翻译： 本发明涉及在转基因有用植物中通过向植物核酸编码具有＆Dgr.6-去饱和酶，6'-延伸酶或者5'-去饱和酶活性的多肽的方法来生产花生四烯酸和/或二十碳五烯酸的方法。 此外，编码ω3-去饱和酶的基因有利地在所述有用植物中表达。 在该方法的另一有利实施方案中，可以在植物中表达编码脂肪酸多肽或脂质代谢生物合成的其它核酸序列。 这里特别有利的核酸序列是那些编码“去饱和酶”，“去饱和酶”，“去饱和酶”，“去饱和酶”，“去饱和酶”，“去饱和酶”和“Dgr”延长酶和/或“ 本发明还涉及根据本发明的方法中生产的油，脂和/或脂肪酸在饲料或食品，化妆品或药物中的用途。
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4.发明申请Method for Producing Arachidonic Acid and/or Eicosapentaenoic Acid in Useful Transgenic Plants 有权在有用的转基因植物中生产花生四烯酸和/或二十碳五烯酸的方法公开(公告)号：US20090234006A1
公开(公告)日：2009-09-17
申请号：US11990244
申请日：2006-08-01
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla , Martin Truksa
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A61K8/361 , A61Q19/00 , C12N9/0083 , C12N9/1029
摘要： The invention relates to a method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants, according to which nucleic acids, which encode polypeptides with Δ-6-desaturase activity, Δ-6-elongase activity or Δ-5-desaturase activity, are introduced into the plants. As an added advantage, a gene that encodes a Δ-3-desaturase is expressed in the useful plants. In an additional embodiment of the method, additional nucleic acid sequences, which encode polypeptides involved in the biosynthesis of the fatty acid or lipid metabolism, can be expressed in the plants. Particularly advantageous nucleic acid sequences are those that encode Δ-8-desaturase activity, Δ-12-desaturase activity, Δ-15-desaturase activity, Δ-4-desaturase activity, Δ-9-elongase activity and/or Δ-5-elongase activity. The invention also relates to the use of the oils, lipids and/or fatty acids that have been produced by said method in animal feed or foodstuffs, cosmetics or pharmaceutical products.
摘要翻译： 本发明涉及在有用的转基因植物中生产花生四烯酸和/或二十碳五烯酸的方法，根据该方法编码具有Δ-6-去饱和酶活性的多肽，Δ-6-延伸酶活性或Δ-5-去饱和酶活性 ，被引入到植物中。 作为另外的优点，编码Δ-3-去饱和酶的基因在有用植物中表达。 在该方法的另外的实施方案中，编码参与脂肪酸或脂质代谢的生物合成的多肽的另外的核酸序列可以在植物中表达。 特别优选的核酸序列是编码Δ-8-去饱和酶活性，Δ-12-去饱和酶活性，Δ-15-去饱和酶活性，Δ-4-去饱和酶活性，Δ-9-延伸酶活性和/或Delta-5- 延长酶活性。 本发明还涉及通过所述方法在动物饲料或食品，化妆品或药物产品中产生的油，脂和/或脂肪酸的用途。
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公开(公告)号：US07473820B2
公开(公告)日：2009-01-06
申请号：US11655545
申请日：2007-01-19
申请人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
发明人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8247
摘要： The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
摘要翻译： 本发明涉及亚麻精蛋白和ω-3去饱和酶基因的启动子。 启动子引导高水平的表达专门在亚麻发育种子。 这种特异性表达模式伴随着储存脂质和蛋白质的生物合成，使得这些启动子特别适用于植物种子中脂肪酸和蛋白质组成的种子特异性修饰。
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公开(公告)号：US20070192902A1
公开(公告)日：2007-08-16
申请号：US11655545
申请日：2007-01-19
申请人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
发明人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8247
摘要： The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
摘要翻译： 本发明涉及亚麻精蛋白和ω-3去饱和酶基因的启动子。 启动子引导高水平的表达专门在亚麻发育种子。 这种特异性表达模式伴随着储存脂质和蛋白质的生物合成，使得这些启动子特别适用于植物种子中脂肪酸和蛋白质组成的种子特异性修饰。
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公开(公告)号：US08841512B2
公开(公告)日：2014-09-23
申请号：US12336184
申请日：2008-12-16
申请人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
发明人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10 , C07H21/04 , C07K14/415
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8247
摘要： The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
摘要翻译： 本发明涉及亚麻精蛋白和ω-3去饱和酶基因的启动子。 启动子引导高水平的表达专门在亚麻发育种子。 这种特异性表达模式伴随着储存脂质和蛋白质的生物合成，使得这些启动子特别适用于植物种子中脂肪酸和蛋白质组成的种子特异性修饰。
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8.发明申请公开(公告)号：US20090172835A1
公开(公告)日：2009-07-02
申请号：US12336184
申请日：2008-12-16
申请人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
发明人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8247
摘要： The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
摘要翻译： 本发明涉及亚麻精蛋白和ω-3去饱和酶基因的启动子。 启动子引导高水平的表达专门在亚麻发育种子。 这种特异性表达模式伴随着储存脂质和蛋白质的生物合成，使得这些启动子特别适用于植物种子中脂肪酸和蛋白质组成的种子特异性修饰。
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公开(公告)号：US07193134B2
公开(公告)日：2007-03-20
申请号：US10165289
申请日：2002-06-06
申请人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
发明人： Xiao Qiu , Martin Truksa , Zhiyuan Hu
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8247
摘要： The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
摘要翻译： 本发明涉及亚麻精蛋白和ω-3去饱和酶基因的启动子。 启动子引导高水平的表达专门在亚麻发育种子。 这种特异性表达模式伴随着储存脂质和蛋白质的生物合成，使得这些启动子特别适用于植物种子中脂肪酸和蛋白质组成的种子特异性修饰。
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