公开(公告)号：US09388436B2

公开(公告)日：2016-07-12

申请号：US13511069

申请日：2010-11-23

申请人： Jörg Bauer ,  Xiao Qiu ,  Patricia Vrinten

发明人： Jörg Bauer ,  Xiao Qiu ,  Patricia Vrinten

IPC分类号： C12P7/64 ,  C12N9/02 ,  C12N15/82

CPC分类号： C12P7/6427 ,  C12N9/0071 ,  C12N15/8247 ,  Y02P20/52

摘要： The present invention relates to nucleic acids derived from Limonomyces roseipellis. The invention also relates to the individual coding sequences and to proteins encoded by these sequences as well to a process for converting oleic acid to linoleic acid to linoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.

摘要翻译： 本发明涉及源自蔷薇属植物的核酸。 本发明还涉及单个编码序列和由这些序列编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法以及在植物中产生花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸。

公开(公告)号：US20130160169A1

公开(公告)日：2013-06-20

申请号：US13330158

申请日：2011-12-19

申请人： Xiao QIU

发明人： Xiao QIU

IPC分类号： C12P7/64 ,  C12N15/52 ,  C12N15/63 ,  C12N15/81 ,  C12N15/80 ,  C12N5/10 ,  C12N15/85 ,  C12N1/21 ,  C12N1/19 ,  C12N1/15 ,  A01H5/00 ,  A01H5/10 ,  C12N15/53 ,  C12N15/82

CPC分类号： C12N9/0071 ,  C12N9/001 ,  C12N9/1029 ,  C12P7/6427 ,  C12Y114/19003

摘要： The invention is directed to isolated polynucleotide and polypeptides of the CoD5, CoD6 and CoE6 genes from Conidiobolus obscurus, the CtE6 gene from Conidiobolus thromboids, and the PgDesX gene from Puccinia graminis; nucleic acid constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same.

摘要翻译： 本发明涉及来自Conidiobolus obscurus的CoD5，CoD6和CoE6基因，来自Conidiobolus thromboids的CtE6基因和来自禾谷镰刀菌的PgDesX基因的分离的多核苷酸和多肽; 核酸构建体，载体和掺入多核苷酸序列的宿主细胞; 以及其制造和使用方法。

公开(公告)号：US08318914B2

公开(公告)日：2012-11-27

申请号：US12671097

申请日：2008-07-30

申请人： Jörg Bauer ,  Xiao Qiu ,  Patricia Vrinten

发明人： Jörg Bauer ,  Xiao Qiu ,  Patricia Vrinten

IPC分类号： C07H21/00 ,  C12N15/74 ,  C12N1/21 ,  C12P21/00 ,  C12P13/06 ,  C12P9/00 ,  C12P7/64 ,  C07K14/00 ,  C07K16/00 ,  A01H5/00

CPC分类号： C12N9/1029 ,  C12N15/8247

摘要： The present invention relates to polynucleotides from Pythium irregulare, Rhizopus oryzae and Euglena gracilis which code for elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

摘要翻译： 本发明涉及编码延长酶并可用于重组生产多不饱和脂肪酸的来自非腐霉的腐霉，米土根霉和小球虫的多核苷酸。 本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。 本发明还涉及针对根据本发明的多肽的抗体。 最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。

公开(公告)号：US20120240248A1

公开(公告)日：2012-09-20

申请号：US13511069

申请日：2010-11-23

申请人： Jörg Bauer ,  Xiao Qiu ,  Patricia Vrinten

发明人： Jörg Bauer ,  Xiao Qiu ,  Patricia Vrinten

IPC分类号： C12P7/64 ,  C12N9/02 ,  C12N1/19 ,  A01H5/10 ,  A01K67/027 ,  C07K16/40 ,  C12N15/53 ,  A01H5/00

CPC分类号： C12P7/6427 ,  C12N9/0071 ,  C12N15/8247 ,  Y02P20/52

摘要： The present invention relates to nucleic acids derived from Limonomyces roseipellis. The invention also relates to the individual coding sequences and to proteins encoded by these sequences as well to a process for converting oleic acid to linoleic acid to linoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.

摘要翻译： 本发明涉及源自蔷薇属植物的核酸。 本发明还涉及单个编码序列和由这些序列编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法以及在植物中产生花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸。

公开(公告)号：US20120094904A1

公开(公告)日：2012-04-19

申请号：US13339428

申请日：2011-12-29

申请人： Xiao Qiu ,  Haiping Hong

发明人： Xiao Qiu ,  Haiping Hong

IPC分类号： A61K38/16 ,  C12N15/63 ,  C12N5/10 ,  C12N1/00 ,  C12N1/11 ,  C12N1/15 ,  C12N1/13 ,  C12P21/06 ,  C07K14/00 ,  C12P7/64 ,  C12N15/82 ,  C12N15/74 ,  A23K1/18 ,  A61P3/10 ,  A61P9/00 ,  A61P35/00 ,  C07H21/04

CPC分类号： C12N15/8247 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  A61K31/202 ,  A61K38/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19003 ,  Y02P20/52

摘要： The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.

摘要翻译： 本发明提供编码新型脂肪酸去饱和酶家族成员的分离的核酸分子。 本发明还提供了含有去饱和酶核酸分子的重组表达载体，已引入表达载体的宿主细胞，以及大规模生产长链多不饱和脂肪酸（LCPUFA）如DHA的方法。

公开(公告)号：US08088906B2

公开(公告)日：2012-01-03

申请号：US13150656

申请日：2011-06-01

申请人： Xiao Qiu ,  Haiping Hong

发明人： Xiao Qiu ,  Haiping Hong

IPC分类号： C07H21/04 ,  C12N1/20 ,  C12N5/10

CPC分类号： C12N15/8247 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  A61K31/202 ,  A61K38/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19003 ,  Y02P20/52

摘要： The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.

摘要翻译： 本发明提供编码新型脂肪酸去饱和酶家族成员的分离的核酸分子。 本发明还提供了含有去饱和酶核酸分子的重组表达载体，已引入表达载体的宿主细胞，以及大规模生产长链多不饱和脂肪酸（LCPUFA）如DHA的方法。

公开(公告)号：US08003853B2

公开(公告)日：2011-08-23

申请号：US11776219

申请日：2007-07-11

申请人： Dauenpen Meesapyodsuk ,  Xiao Qiu

发明人： Dauenpen Meesapyodsuk ,  Xiao Qiu

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N1/20 ,  C12N5/10 ,  C07H21/04

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23K20/189 ,  A23L33/115 ,  A23L33/12 ,  A61K36/185 ,  A61K36/28 ,  A61K36/286 ,  A61K36/31 ,  A61K36/47 ,  A61K36/48 ,  A61K36/535 ,  A61K36/55 ,  A61K36/63 ,  A61K36/66 ,  A61K36/889 ,  C12N9/0083 ,  C12N15/8247 ,  C12P7/6472

摘要： The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., SDA, EPA and DHA.

摘要翻译： 本发明提供编码新型脂肪酸去饱和酶家族成员的分离的核酸分子。 本发明还提供含有去饱和酶核酸分子的重组表达载体，已经引入表达载体的宿主细胞，以及大规模生产长链多不饱和脂肪酸（LCPUFA）如SDA，EPA和DHA的方法。

公开(公告)号：US20110162105A1

公开(公告)日：2011-06-30

申请号：US13060458

申请日：2009-08-25

申请人： Jörg Bauer ,  Toralf Senger ,  Thorsten Zank ,  Xiao Qiu ,  Guohai Wu

发明人： Jörg Bauer ,  Toralf Senger ,  Thorsten Zank ,  Xiao Qiu ,  Guohai Wu

IPC分类号： A01H1/06 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12N1/19 ,  C12N1/15 ,  A01H5/00 ,  A01H5/10 ,  C12N9/02 ,  C12P7/64 ,  C07C57/03

CPC分类号： C12N15/8247 ,  C12N5/14 ,  C12N9/00 ,  C12N9/0004

摘要： The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.

摘要翻译： 本发明涉及来自Drechslera tritici-repentis，Cylindorcarpon herteronema，Diploida natalensis，Stagonospora nodorum，Microdochium nivalae和Periplaneta americana的核酸。 本发明还涉及个体编码序列和由这些序列与其它序列组合编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法，以及花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸的生产 酸在植物中。

公开(公告)号：US20080155705A1

公开(公告)日：2008-06-26

申请号：US10566944

申请日：2004-07-16

申请人： Thorsten Zank ,  Jorg Bauer ,  Petra Cirpus ,  Amine Abbadi ,  Ernst Heinz ,  Xiao Qiu ,  Patricia Vrinten ,  Petra Sperling ,  Frederic Domergue ,  Astrid Meyer ,  Jelena Kirsch

发明人： Thorsten Zank ,  Jorg Bauer ,  Petra Cirpus ,  Amine Abbadi ,  Ernst Heinz ,  Xiao Qiu ,  Patricia Vrinten ,  Petra Sperling ,  Frederic Domergue ,  Astrid Meyer ,  Jelena Kirsch

IPC分类号： A01K67/00 ,  C12N15/82 ,  C07C51/00 ,  C12N15/11 ,  A01H5/00 ,  C12N15/00 ,  C12N1/20

CPC分类号： C12N15/8247 ,  A23D9/00 ,  A23D9/02 ,  A23L33/115 ,  A23V2002/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/0093 ,  C12N9/1029 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12Y114/19 ,  C12Y114/19003 ,  C12Y203/01199 ,  Y02P20/52

摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

公开(公告)号：US20070192902A1

公开(公告)日：2007-08-16

申请号：US11655545

申请日：2007-01-19

申请人： Xiao Qiu ,  Martin Truksa ,  Zhiyuan Hu

发明人： Xiao Qiu ,  Martin Truksa ,  Zhiyuan Hu

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8234 ,  C07K14/415 ,  C12N15/8247

摘要： The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.

摘要翻译： 本发明涉及亚麻精蛋白和ω-3去饱和酶基因的启动子。 启动子引导高水平的表达专门在亚麻发育种子。 这种特异性表达模式伴随着储存脂质和蛋白质的生物合成，使得这些启动子特别适用于植物种子中脂肪酸和蛋白质组成的种子特异性修饰。