公开(公告)号：US20250067705A1

公开(公告)日：2025-02-27

申请号：US18294759

申请日：2022-06-13

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Young-Ha Hwang ,  Henry Julian Hinton ,  Han Sae Jung ,  Woo-Bin Jung

IPC: G01N27/416

Abstract: An electrochemical apparatus includes an array of pixels disposed on a chip, stimulator circuitry disposed on the chip and configured to provide electrical input signals to cause stimulation of the pixels of the array, and sensor circuitry disposed on the chip and configured to read electrical output signals from the pixels of the array. The stimulator circuitry is configured to provide the input signals to cause stimulation of the pixels individually, and the sensor circuitry is configured to selectively read the output signals from the pixels while the pixels are being stimulated. The sensor circuitry is configured to measure an open-circuit voltage at each of the pixels and a current flow at each of the pixels while the pixels are being stimulated by the stimulator circuitry. The open-circuit voltage may be measured while the current flow is being measured.

公开(公告)号：US20250160718A1

公开(公告)日：2025-05-22

申请号：US18839599

申请日：2023-03-24

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Jun Wang ,  Woo-Bin Jung ,  Hongkun Park

IPC: A61B5/294

Abstract: A device is provided for performing an electrical assessment in a biological environment. The device includes a substrate having an exposed surface having holes. At least one of the holes includes surfaces coated with a conductive material that is in electrical contact with an electrode. The device also includes circuitry controllable to apply a stimulus to the conductive material of the hole by applying the stimulus to the electrode in electrical contact with the hole. The hole may be disposed in a well structure that includes a wall surrounding the hole. The well structure may have a dimension such that a cell of interest in the biological environment is able to cover an entirety of a rim of the wall of the well structure. The wall of the well structure may surround one or more of the holes, which may be in electrical contact with a same electrode.

公开(公告)号：US20240280535A1

公开(公告)日：2024-08-22

申请号：US18569809

申请日：2022-06-13

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Woo-Bin Jung ,  Han Sae Jung ,  Jun Wang ,  Young-Ha Hwang ,  Henry Julian Hinton ,  Jeffrey T. Abbott ,  Hongkun Park

IPC: G01N27/416 ,  G01N27/27 ,  G01N27/30

CPC classification number: G01N27/4167 ,  G01N27/27 ,  G01N27/302

Abstract: Devices and methods for controlling the local pH of solutions (e.g., for parallelized polymer synthesis) are generally described. These may offer several advantages, including the ability to control pH using a plurality of pixels, and/or the ability to sense the pH associated with each pixel, according to certain embodiments. In some embodiments, such devices are used to selectively synthesize polymer sequences (e.g., DNA sequences) associated with each pixel. The pixels can, in some embodiments, comprise electrodes that can apply an electrical potential or current to a solution comprising an electrically sensitive pH modifier. In some cases, reaction of the electrically sensitive pH modifier may cause a change in pH. The pixels may comprise circuit components that can operate in multiple modes (e.g. as potentiostats, galvanostats, or open-circuit potential sensors capable of sensing local pH).