公开(公告)号：US20020086326A1

公开(公告)日：2002-07-04

申请号：US10041890

申请日：2002-01-07

申请人： Promega Corporation

发明人： Craig E. Smith ,  Charles K. York

IPC分类号： C12Q001/68 ,  C12N015/00

CPC分类号： C12N15/1013 ,  C12Q1/6806 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/916 ,  Y10S977/92 ,  Y10S977/924 ,  Y10S977/96

摘要： The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the biological target material in a mixture of the medium and particles, separating the complex from the mixture using external magnetic force, and eluting the biological target material from the complex. The preferred embodiments of magnetic silica particles used in the methods and kits of the present invention are capable of forming a complex with at least 2 nullg of biological target material per milligram of particle, and of releasing at least 60% of the material from the complex in the elution step of the method. The methods of the present invention produce isolated biological target material which is substantially free of contaminants, such as metals or macromolecular substances, which can interfere with further processing or analysis, if present.

摘要翻译： 本发明提供了使用二氧化硅磁性颗粒从介质中的其他物质分离生物靶材料，特别是DNA或RNA或DNA和RNA的混合分子的方法。 本发明的方法包括在介质和颗粒的混合物中形成二氧化硅磁性颗粒和生物目标材料的复合物，使用外部磁力从混合物中分离复合物，并从复合物中洗脱生物目标物质。 在本发明的方法和试剂盒中使用的磁性二氧化硅颗粒的优选实施方案能够与每毫克颗粒至少2个生物靶材料形成复合物，并且从复合物中释放至少60％的材料 在该方法的洗脱步骤中。 本发明的方法产生基本上不含污染物（例如金属或大分子物质）的分离的生物靶材料，如果存在可能会干扰进一步的加工或分析。