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    SYSTEMS AND METHODS FOR PROTEOMIC ACTIVITY ANALYSIS USING DNA-ENCODED PROBES
    1.
    发明申请
    SYSTEMS AND METHODS FOR PROTEOMIC ACTIVITY ANALYSIS USING DNA-ENCODED PROBES 有权

    公开(公告)号：US20210269863A1

    公开(公告)日：2021-09-02

    申请号：US17323695

    申请日：2021-05-18

    申请人： Purdue Research Foundation

    发明人： Casey J. Krusemark ,  Kyle Denton ,  Dongwook Kim ,  Rachael Jetson

    IPC分类号： C12Q1/6823 ,  C12N15/10 ,  C07H21/04 ,  C12Q1/686 ,  G01N33/68

    摘要： Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.

    AFFINITY LABELING OF DNA-LINKED LIGANDS FOR HIGH THROUGHPUT LIGAND BINDING ASSAYS AND THE USES THEREOF
    2.
    发明申请
    AFFINITY LABELING OF DNA-LINKED LIGANDS FOR HIGH THROUGHPUT LIGAND BINDING ASSAYS AND THE USES THEREOF 有权

    公开(公告)号：US20220098643A1

    公开(公告)日：2022-03-31

    申请号：US17472756

    申请日：2021-09-13

    申请人： Purdue Research Foundation

    发明人： Casey J. Krusemark ,  Bo Cai

    IPC分类号： C12Q1/686 ,  C12Q1/48

    摘要： The present application relates to an assay method using affinity crosslinking of DNA-linked ligands to proteins to enable small molecule screening and determination of apparent affinity constants of ligands to the proteins. This method has been applied to determine 96 compounds' dissociation constants to a protein target simultaneously, and directly determine a compound's IC50 against five protein targets concurrently in crude cell lysates. Additionally, this approach was used to screen a Library of Pharmacologically Active Compounds (LOPAC) library against dihydrofolate reductase (eDHFR), enabling the discovery of a novel eDHFR inhibitor (IC50=7.9 μM). An assay kit and the method of uses are within the scope of this disclosure.

    Systems and methods for proteomic activity analysis using DNA-encoded probes
    5.
    发明授权
    Systems and methods for proteomic activity analysis using DNA-encoded probes 有权

    公开(公告)号：US11028427B2

    公开(公告)日：2021-06-08

    申请号：US15753317

    申请日：2016-08-18

    申请人： Purdue Research Foundation

    发明人： Casey J. Krusemark ,  Kyle E. Denton ,  Dongwook Kim ,  Rachael R. Jetson

    IPC分类号： C12Q1/6823 ,  C12N15/10 ,  C07H21/04 ,  C12Q1/686 ,  G01N33/68

    摘要： Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.