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公开(公告)号：US07605140B2
公开(公告)日：2009-10-20
申请号：US11115922
申请日：2005-04-26
申请人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
发明人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
IPC分类号： A61K48/00
CPC分类号： A61K48/005 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/52 , C07K2319/00 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2799/021 , C12N2810/85 , C12N2830/008
摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
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公开(公告)号：US20060024726A1
公开(公告)日：2006-02-02
申请号：US11202009
申请日：2005-08-11
申请人： Qiang Liu , Edward Rebar , Andrew Jamieson
发明人： Qiang Liu , Edward Rebar , Andrew Jamieson
CPC分类号： C07K14/00 , C07K1/00 , C07K14/47 , C07K14/4702 , C07K2299/00 , C12N15/102 , C12N15/62 , C12Q1/6886 , C12Q2600/156 , G01N33/5308 , G01N33/57484 , G01N33/6875 , G01N2500/04
摘要： The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
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公开(公告)号：US08071564B2
公开(公告)日：2011-12-06
申请号：US12553826
申请日：2009-09-03
申请人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
发明人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
CPC分类号： A61K48/005 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/52 , C07K2319/00 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2799/021 , C12N2810/85 , C12N2830/008
摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。
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公开(公告)号：US06794136B1
公开(公告)日：2004-09-21
申请号：US09716637
申请日：2000-11-20
申请人： Stephen P. Eisenberg , Qiang Liu , Andrew Jamieson , Edward Rebar
发明人： Stephen P. Eisenberg , Qiang Liu , Andrew Jamieson , Edward Rebar
IPC分类号： C12Q168
CPC分类号： C07K14/4702 , C12N15/102
摘要： Disclosed herein are design methods for optimizing the specificity of a binding protein. The methods comprise iterative cycles of rational design, site selection, redesign and site selection of the redesigned molecule.
摘要翻译： 本文公开了用于优化结合蛋白的特异性的设计方法。 该方法包括重新设计的分子的合理设计，位点选择，重新设计和位点选择的迭代循环。
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公开(公告)号：US07560440B2
公开(公告)日：2009-07-14
申请号：US11486994
申请日：2006-07-14
申请人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
发明人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
IPC分类号： A61K48/00
CPC分类号： A61K48/005 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/52 , C07K2319/00 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2799/021 , C12N2810/85 , C12N2830/008
摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。
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公开(公告)号：US20060019349A1
公开(公告)日：2006-01-26
申请号：US11225686
申请日：2005-09-12
申请人： Qiang Liu , Edward Rebar , Andrew Jamieson
发明人： Qiang Liu , Edward Rebar , Andrew Jamieson
CPC分类号： C07K14/00 , C07K1/00 , C07K14/47 , C07K14/4702 , C07K2299/00 , C12N15/102 , C12N15/62 , C12Q1/6886 , C12Q2600/156 , G01N33/5308 , G01N33/57484 , G01N33/6875 , G01N2500/04
摘要： The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
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7.发明授权Compositions and methods for regulation of plant gamma-tocopherol methyltransferase 有权用于调节植物γ-生育酚甲基转移酶的组合物和方法公开(公告)号：US07947873B2
公开(公告)日：2011-05-24
申请号：US10418552
申请日：2003-04-17
申请人： Guofu Li , Qiang Liu , Andrew Jamieson , Edward Rebar , Mylavarapu Venkatramesh
发明人： Guofu Li , Qiang Liu , Andrew Jamieson , Edward Rebar , Mylavarapu Venkatramesh
CPC分类号： C12N9/1007 , C07K14/415 , C07K14/4702 , C07K2319/00 , C12N15/8243 , C12N15/8247 , C12P17/06
摘要： Disclosed herein are zinc finger proteins that bind to target sites in a plant gamma-tocopherol methyl transferase (GMT) gene; compositions comprising these GMT-targeted zinc finger proteins and methods of making and using such zinc finger proteins. Also disclosed are methods for modulating the alpha-tocopherol content in various plant organs, particularly seeds, in transgenic plants.
摘要翻译： 本文公开了结合植物γ-生育酚甲基转移酶（GMT）基因中的靶位点的锌指蛋白; 包含这些GMT靶向锌指蛋白的组合物以及制备和使用这种锌指蛋白的方法。 还公开了用于在转基因植物中调节各种植物器官，特别是种子中的α-生育酚含量的方法。
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公开(公告)号：US20100093838A1
公开(公告)日：2010-04-15
申请号：US12553826
申请日：2009-09-03
申请人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
发明人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
IPC分类号： A61K31/7088 , A61P9/00
CPC分类号： A61K48/005 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/52 , C07K2319/00 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2799/021 , C12N2810/85 , C12N2830/008
摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。
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公开(公告)号：US20070072818A1
公开(公告)日：2007-03-29
申请号：US11486994
申请日：2006-07-14
申请人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen Eisenberg , Eric Jarvis
发明人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen Eisenberg , Eric Jarvis
IPC分类号： A61K48/00
CPC分类号： A61K48/005 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/52 , C07K2319/00 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2799/021 , C12N2810/85 , C12N2830/008
摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。
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公开(公告)号：US07067317B2
公开(公告)日：2006-06-27
申请号：US09846033
申请日：2001-04-30
申请人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
发明人： Edward Rebar , Andrew Jamieson , Qiang Liu , Pei-Qi Liu , Alan Wolffe , Stephen P. Eisenberg , Eric Jarvis
CPC分类号： C07K14/52 , A61K38/00 , A61K48/00 , C07K14/47 , C07K2319/00 , C12N2799/021
摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
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