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公开(公告)号：US4843002A
公开(公告)日：1989-06-27
申请号：US655180
申请日：1984-09-27
CPC分类号： C12N15/76 , C12N15/65 , C12N9/1029 , Y10S435/872 , Y10S435/886 , Y10S435/889 , Y10S435/896
摘要： A novel method of selecting Streptomyces recombinant DNA-containing host cells and vectors useful in exemplifying the method are described. The vectors confer apramycin resistance to sensitive Streptomyces host cells and thus provide a convenient method of selecting Streptomyces transformants. The apramycin resistance-conferring gene used in the method is an acetyltransferase aac(3)IV gene and can be isolated from E. coli K12 BE1041/pKC309 (NRRL B-15827) on an .about.1.5 kb PstI-EcoRI restriction fragment.
摘要翻译： 描述了选择含有重链DNA的宿主细胞的新方法和用于举例说明该方法的载体。 这些载体赋予了对敏感链霉菌宿主细胞的安普霉素抗性，从而提供了选择链霉菌转化体的方便的方法。 该方法中使用的安普霉素抗性基因是乙酰转移酶aac（3）IV基因，并且可以在DIFFERENCE 1.5kb PstI-EcoRI限制性片段上从大肠杆菌K12 BE1041 / pKC309（NRRL B-15827）中分离。
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公开(公告)号：US4921801A
公开(公告)日：1990-05-01
申请号：US842102
申请日：1986-03-20
CPC分类号： C12N15/76 , Y10S435/849 , Y10S435/889
摘要： Novel recombinant DNA cosmid shuttle vectors and a method of using them in the construction of genomic DNA libraries are described. The vectors demonstrate the incorporation of both the size selection and in vitro packaging mechanisms of lambda into a Streptomyces-E. coli shuttle vector by the incorporation of two or more COS sequences of bacteriophage lambda.
摘要翻译： 描述了新的重组DNA粘粒穿梭载体及其在构建基因组DNA文库中的使用方法。 这些载体证明将λ的大小选择和体外包装机制纳入Streptomyces-E中。 大肠杆菌穿梭载体通过结合两个或多个COS序列的噬菌体λ。
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公开(公告)号：US4935340A
公开(公告)日：1990-06-19
申请号：US742349
申请日：1985-06-07
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N15/52 , C12N15/76 , C12Q1/68 , C12R1/03 , C12R1/045 , C12R1/07 , C12R1/15 , C12R1/29 , C12R1/365 , C12R1/465 , C12R1/625 , C12R1/745 , C12R1/80 , G01N33/50
摘要： The present invention is a method for isolating antibiotic biosynthetic genes. To practice the method, an antibiotic resistance-conferring DNA segment is labelled and used as a probe to find, via DNA hydridization, homologous DNA in a genetic library which comprises chromosomal and plasmid DNA of an antibiotic-producing organism. Individual vectors of the genetic library which hybridize to the antibiotic resistance-conferring gene, and which comprise .about.1-45 kb of contiguous DNA from the antibiotic-producing organism, which also comprise an antibiotic biosynthetic gene. The present method is exemplified by using the erythromycin resistance-conferring gene of Streptomyces erythreus to clone the erythromycin biosynthetic pathway from the same organism. The erythromycin biosynthetic pathway isolated with the present method synthesizes erythromycin when introduced into S. lividans TK23.
摘要翻译： 本发明是分离抗生素生物合成基因的方法。 为了实施该方法，将抗生素抗性赋予DNA片段标记并用作探针，通过DNA杂交在包含产生抗生素的生物体的染色体和质粒DNA的遗传文库中发现同源DNA。 与抗生素耐药基因杂交的基因文库的单个载体，其包含来自产生抗生素的生物体的差异1-45kb的连续DNA，其也包含抗生素生物合成基因。 本发明的方法通过使用红霉素抗性赋予基因，从相同的生物体克隆红霉素生物合成途径。 用本发明方法分离的红霉素生物合成途径将红霉素引入青光眼链霉菌TK23后合成红霉素。
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公开(公告)号：US5586501A
公开(公告)日：1996-12-24
申请号：US547947
申请日：1995-10-25
CPC分类号： A43B1/0027 , A43B3/0078 , B41M1/12 , B41M3/005
摘要： A disappearing ink marking system adaptable to a sole of a user that includes an upper layer, an intermediate layer affixed to the upper layer, a lower layer affixed to the intermediate layer, a reservoir tube passing into the intermediate layer and in fluid communication therewith, disappearing ink, and at least one image block affixed to the lower layer and in fluid communication with the intermediate layer. Disappearing ink flowing through the reservoir tube escapes through the plurality of reservoir tube apertures and enters the intermediate layer and is absorbed therein where upon when pressure is applied to the intermediate layer and the at least one image block the disappearing ink is forced from the intermediate layer through the at least one lower layer aperture and into the at least one image block which in turn causes the at least one image block to disperse the disappearing ink and leave a temporary image.
摘要翻译： 一种适用于使用者鞋底的消失油墨标记系统，包括上层，固定在上层的中间层，固定在中间层上的下层，进入中间层并与之流体连通的储存管， 消失的墨水和至少一个图像块固定到下层并与中间层流体连通。 流过储存器管的消失油墨通过多个储存器管孔逸出并进入中间层并被吸收在其中，当压力施加到中间层和至少一个图像块时，消失的油墨被迫从中间层 通过所述至少一个下层孔径并进入所述至少一个图像块，所述至少一个图像块又导致所述至少一个图像块分散所述消失的墨水并且留下临时图像。
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公开(公告)号：US5393665A
公开(公告)日：1995-02-28
申请号：US833596
申请日：1992-02-10
CPC分类号： C12N15/76
摘要： Novel vectors and methods for a single-stranded DNA mediated gene transfer system via transformation, fusion or transduction of Streptomyces, other actinomycetes, and E. coli using a variety of vectors. Phasmid shuttle vectors of the invention are particularly useful as single-stranded vectors that appear to bypass one or more host cell restriction systems, and thus increase the efficiency of gene transfer into highly restrictive host cell systems. New and useful vectors are provided that allow for the cloning of genes both for increasing the yields of known antibiotics and also for producing new antibiotics, antibiotic derivatives, or any other useful gene product, including a variety of mammalian protein products.
摘要翻译： 通过使用多种载体转化，融合或转导链霉菌，其他放线菌和大肠杆菌的单链DNA介导的基因转移系统的新型载体和方法。 本发明的噬粒穿梭载体特别可用作看起来绕过一个或多个宿主细胞限制系统的单链载体，从而提高基因转移到高度限制性的宿主细胞系统中的效率。 提供了新的和有用的载体，其允许克隆基因以增加已知抗生素的产量，并且还用于生产新的抗生素，抗生素衍生物或任何其它有用的基因产物，包括多种哺乳动物蛋白质产物。
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6.发明授权Picromycin resistance-conferring gene, designated pica, for use in streptomyces and other organisms 失效PICROMYCIN RESISTANCE-CONFERRING GENE，指定PICA，用于结构和其他有机体
公开(公告)号：US5057425A
公开(公告)日：1991-10-15
申请号：US226360
申请日：1988-07-29
申请人： Richard K. Stanzak
发明人： Richard K. Stanzak
CPC分类号： C12N15/65 , C12N15/76 , Y10S435/886
摘要： A novel gene conferring resistance to picromycin in Streptomyces and related organisms was cloned from a genomic library of Streptomyces felleus DNA. The novel picromycin resistance-conferring gene can be isolated on an .about.2.8 kb PvuII fragment by subcloning the restriction fragment from plasmid pOJ321. This PvuII fragment contains all of the information required for the expression of the picromycin resistant phenotype in Streptomyces. Methods for inducing resistance, as well as vectors and transformants containing the novel picromycin resistance gene, are provided.
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