公开(公告)号：US20050158842A1

公开(公告)日：2005-07-21

申请号：US11011979

申请日：2004-12-13

申请人： R. Yocum ,  Thomas Patterson

发明人： R. Yocum ,  Thomas Patterson

IPC分类号： C12N9/12 ,  C12N15/52 ,  C12N15/54 ,  C12P13/02 ,  C12P17/04 ,  C12N1/21 ,  C07H21/04 ,  C12N15/74

CPC分类号： C12N9/1205 ,  C12N15/52 ,  C12P13/02 ,  C12P17/04

摘要： The present invention features methods for the identification of compounds and compositions useful as antibiotics and antibacterial agents. In particular, the invention features methods for the identification of modulators of a previously unidentified target protein, termed CoaX. High-throughput assay systems are featured as well as assay kits for the identification of CoaX modulators. Also featured are coaX nucleic acid molecules and purified CoaX proteins, as well as recombinant vectors and microorganisms including the gene, coaX.

摘要翻译： 本发明的特征在于用于鉴定可用作抗生素和抗菌剂的化合物和组合物的方法。 特别地，本发明的特征在于用于鉴定称为CoaX的先前未鉴定的靶蛋白的调节剂的方法。 具有高通量测定系统以及用于鉴定CoaX调节剂的测定试剂盒。 还具有coaX核酸分子和纯化的CoaX蛋白，以及重组载体和微生物，包括该基因，coaX。

公开(公告)号：US20050089973A1

公开(公告)日：2005-04-28

申请号：US10984449

申请日：2004-11-08

申请人： R. Yocum ,  Thomas Patterson ,  Theron Hermann ,  Janice Pero

发明人： R. Yocum ,  Thomas Patterson ,  Theron Hermann ,  Janice Pero

IPC分类号： C12N1/21 ,  C12N15/52 ,  C12P13/02 ,  C12P17/04 ,  C12P13/04 ,  C12P13/06

CPC分类号： C12P17/04 ,  C12N15/52 ,  C12P13/02

摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

摘要翻译： 本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。 本发明提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。 描述了以前体非依赖性方式和高产率生产泛化合物的方法。 还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。 本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。 重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。 还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。

公开(公告)号：US20070196903A1

公开(公告)日：2007-08-23

申请号：US11786542

申请日：2007-04-11

申请人： Scott Van Arsdell ,  R. Yocum ,  John Perkins ,  Janice Pero

发明人： Scott Van Arsdell ,  R. Yocum ,  John Perkins ,  Janice Pero

IPC分类号： C12P17/18 ,  C12N9/10 ,  C12N1/21

CPC分类号： C07D495/04 ,  C12P17/186 ,  C12R1/125

摘要： A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. The method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.

摘要翻译： 公开了一种使用产生赖氨酸利用DAPA氨基转移酶的细菌来增加生物素和生物素前体脱硫生物素生产的方法。 该方法涉及使用在赖氨酸存在下生长或在对赖氨酸生物合成失调的细菌。