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    Microorganisms and processes for enhanced production of pantothenate
    1.
    发明申请
    Microorganisms and processes for enhanced production of pantothenate 审中-公开
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US20060099692A1

    公开(公告)日：2006-05-11

    申请号：US10520210

    申请日：2002-07-03

    申请人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    发明人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    IPC分类号： C12P19/12 ,  C12P13/04 ,  C12N1/21

    CPC分类号： C07H21/04 ,  C12N9/0006 ,  C12N9/1003 ,  C12N9/1014 ,  C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    Microorganisms and processes for enhanced production of pantothenate
    2.
    发明申请
    Microorganisms and processes for enhanced production of pantothenate 有权
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US20060141585A1

    公开(公告)日：2006-06-29

    申请号：US10520220

    申请日：2003-07-03

    申请人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    发明人： R Yocum ,  Thomas Patterson ,  Janice Pero ,  Theron Hermann

    IPC分类号： C12P13/04 ,  C12N1/21

    CPC分类号： C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    Microorganisms and assays for the identification of antibiotics
    3.
    发明申请
    Microorganisms and assays for the identification of antibiotics 审中-公开
    用于鉴定抗生素的微生物和测定

    公开(公告)号：US20050158842A1

    公开(公告)日：2005-07-21

    申请号：US11011979

    申请日：2004-12-13

    申请人： R. Yocum ,  Thomas Patterson

    发明人： R. Yocum ,  Thomas Patterson

    IPC分类号： C12N9/12 ,  C12N15/52 ,  C12N15/54 ,  C12P13/02 ,  C12P17/04 ,  C12N1/21 ,  C07H21/04 ,  C12N15/74

    CPC分类号： C12N9/1205 ,  C12N15/52 ,  C12P13/02 ,  C12P17/04

    摘要： The present invention features methods for the identification of compounds and compositions useful as antibiotics and antibacterial agents. In particular, the invention features methods for the identification of modulators of a previously unidentified target protein, termed CoaX. High-throughput assay systems are featured as well as assay kits for the identification of CoaX modulators. Also featured are coaX nucleic acid molecules and purified CoaX proteins, as well as recombinant vectors and microorganisms including the gene, coaX.

    摘要翻译： 本发明的特征在于用于鉴定可用作抗生素和抗菌剂的化合物和组合物的方法。 特别地，本发明的特征在于用于鉴定称为CoaX的先前未鉴定的靶蛋白的调节剂的方法。 具有高通量测定系统以及用于鉴定CoaX调节剂的测定试剂盒。 还具有coaX核酸分子和纯化的CoaX蛋白，以及重组载体和微生物，包括该基因，coaX。

    Methods and microorganisms for production of panto-compounds
    4.
    发明申请
    Methods and microorganisms for production of panto-compounds 有权
    泛化合物生产方法和微生物

    公开(公告)号：US20050089973A1

    公开(公告)日：2005-04-28

    申请号：US10984449

    申请日：2004-11-08

    申请人： R. Yocum ,  Thomas Patterson ,  Theron Hermann ,  Janice Pero

    发明人： R. Yocum ,  Thomas Patterson ,  Theron Hermann ,  Janice Pero

    IPC分类号： C12N1/21 ,  C12N15/52 ,  C12P13/02 ,  C12P17/04 ,  C12P13/04 ,  C12P13/06

    CPC分类号： C12P17/04 ,  C12N15/52 ,  C12P13/02

    摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

    摘要翻译： 本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。 本发明提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。 描述了以前体非依赖性方式和高产率生产泛化合物的方法。 还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。 本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。 重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。 还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。

    System and method for downhole geothermal electrical power generation
    6.
    发明授权
    System and method for downhole geothermal electrical power generation 有权
    井下地热发电系统及方法

    公开(公告)号：US08610303B2

    公开(公告)日：2013-12-17

    申请号：US13343516

    申请日：2012-01-04

    申请人： John R. Yocum, Jr.

    发明人： John R. Yocum, Jr.

    IPC分类号： F03B13/00

    CPC分类号： F03B13/02

    摘要： There is provided herein a system and method for generating downhole electricity from wells or similar apertures that penetrate sufficiently deep into the subsurface to allow liquid water to be converted to steam. In the preferred embodiment, a well that reaches to a point in the subsurface where the ambient temperature at depth is significantly above the boiling point of water (i.e., greater than 212° F.) will be used, said steam providing the force necessary to turn the blades of a turbine which, in turn, provides rotational force a downhole generator, thereby resulting in the generation of electricity.

    摘要翻译： 这里提供了用于从井或相似的孔产生井下电力的系统和方法，其充分渗透到地下以允许液态水转化为蒸汽。 在优选实施例中，将使用达到深度周围环境温度明显高于水沸点（即大于212°F）的地下点的井，所述蒸汽提供所需的力 转动涡轮机的叶片，其又提供旋转力井下发电机，从而导致电力的产生。

    Microorganisms and processes for enhanced production of pantothenate
    7.
    发明授权
    Microorganisms and processes for enhanced production of pantothenate 有权
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US07989187B2

    公开(公告)日：2011-08-02

    申请号：US10520220

    申请日：2003-07-03

    申请人： Rogers R. Yocum ,  Thomas A. Patterson ,  Janice G. Pero ,  Theron Hermann

    发明人： Rogers R. Yocum ,  Thomas A. Patterson ,  Janice G. Pero ,  Theron Hermann

    IPC分类号： C12P13/04 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    Methods and organisms for production of b6 vitamers
    10.
    发明申请
    Methods and organisms for production of b6 vitamers 审中-公开
    生产b6维生素的方法和生物

    公开(公告)号：US20050164335A1

    公开(公告)日：2005-07-28

    申请号：US10508768

    申请日：2003-03-21

    申请人： R Yocum ,  Mark Williams ,  Janice Pero

    发明人： R Yocum ,  Mark Williams ,  Janice Pero

    IPC分类号： C12N1/12 ,  C12N1/18 ,  C12N1/21 ,  C12N9/00 ,  C12N9/10 ,  C12N15/52 ,  C12P17/12 ,  C12P25/00

    CPC分类号： C12N9/00 ,  C12N15/52 ,  C12P17/12

    摘要： The present invention features methods of producing B6 vitamers that involve culturing an organism overexpressing an enzyme that catalyzes a step in the biosynthesis of a B6 vitamer under conditions such that a B6 vitamer is produced. The present invention further features methods of producing B6 vitamers that involve culturing recombinant microorganisms having increased activity of at least one B6 vitamer biosynthetic enzyme, e.g., YaaD or YaaE, or a homologue thereof, or Epd, PdxA, PdxJ, PdxF, PdxB, PdxH, and/or Dxs, or a homologue thereof.

    摘要翻译： 本发明的特征在于生产B6维生素的方法，其涉及培养在产生B6维生素的条件下过量表达催化B6维生素生物合成步骤的酶的生物体的培养。 本发明还涉及生产B6维生素的方法，其涉及培养具有增加至少一种B6维生素生物合成酶的活性的重组微生物，例如YaaD或YaaE或其同源物，或Epd，PdxA，PdxJ，PdxF，PdxB，PdxH ，和/或Dxs，或其同源物。