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    Efficient Selectivity of Recombinant Proteins
    4.
    发明申请
    Efficient Selectivity of Recombinant Proteins 有权
    重组蛋白的高效选择性

    公开(公告)号：US20160053280A1

    公开(公告)日：2016-02-25

    申请号：US14829834

    申请日：2015-08-19

    Applicant: REGENERON PHARMACEUTICALS, INC.

    Inventor： Dipali DESHPANDE ,  Darya BURAKOV ,  Gang CHEN ,  James FANDL

    IPC: C12N15/85 ,  C07K16/00 ,  C12P21/00

    CPC classification number: C12N15/85 ,  C07K2319/30 ,  C12P21/005

    Abstract: The invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems.

    Abstract translation: 本发明提供了一种新的表达系统，其包括促进糖蛋白的期望的翻译后修饰的哺乳动物选择性标记。 特别地，本发明包括通过使用基于哺乳动物来源的GPT基因的选择标记系统来在哺乳动物细胞中优化重组蛋白表达的方法和组合物。 本发明包括促进哺乳动物细胞中重组蛋白质的选择性和增强的表达拷贝以及蛋白质产量的方法，以及使用GPT表达系统的方法。

    INDUCIBLE EUKARYOTIC EXPRESSION SYSTEM
    5.
    发明申请
    INDUCIBLE EUKARYOTIC EXPRESSION SYSTEM 有权
    可诱导的真核表达系统

    公开(公告)号：US20140315249A1

    公开(公告)日：2014-10-23

    申请号：US14182863

    申请日：2014-02-18

    Applicant: REGENERON PHARMACEUTICALS, INC.

    Inventor： Changlin DOU ,  Gang CHEN ,  James P. FANDL

    IPC: C12N15/63 ,  C07K16/00

    CPC classification number: C12N15/635 ,  A01K2217/05 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2319/00 ,  C12N15/63 ,  C12N15/85 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/42 ,  C12N2840/20 ,  C12P21/00

    Abstract: Compositions and methods for the inducible expression of genes in eukaryotic cells are provided. Expression of a nucleotide sequence of interest encoding a protein of interest is controlled by a regulatory fusion protein that consists of a transcription blocking domain and a ligand-binding domain. When a cognate ligand for the ligand-binding domain is present, transcription of the nucleotide sequence of interest is blocked. Upon removal of the cognate ligand, the nucleotide sequence of interest is transcribed. The method is useful for large scale bioreactor production of a desired protein of interest in eukaryotic cells.

    Abstract translation: 提供了在真核细胞中可诱导表达基因的组合物和方法。 通过由转录阻断结构域和配体结合结构域组成的调节融合蛋白来控制感兴趣的蛋白质的目标核苷酸序列的表达。 当存在配体结合结构域的同源配体时，感兴趣的核苷酸序列的转录被阻断。 去除同源配体后，转录目的核苷酸序列。 该方法可用于在真核细胞中大规模生物反应器生产期望的蛋白质。

    METHOD FOR GENERATING HIGH AFFINITY ANTIBODIES
    8.
    发明申请
    METHOD FOR GENERATING HIGH AFFINITY ANTIBODIES 审中-公开

    公开(公告)号：US20200369783A1

    公开(公告)日：2020-11-26

    申请号：US16987639

    申请日：2020-08-07

    Applicant: Regeneron Pharmaceuticals, Inc.

    Inventor： Gang CHEN ,  Robert BABB ,  James P. FANDEL

    IPC: C07K16/32 ,  C07K16/00 ,  C07K16/22 ,  G01N33/50

    Abstract: The present disclosure relates to a method for identifying cells that express antigen-specific antibodies with a high binding affinity for a monomeric antigen. Using fluorescence activated cell sorting, cells expressing high affinity antigen-specific antibodies are selected from a population of immune cells isolated from a mammal that has been immunized with or otherwise exposed to the antigen. Nucleic acids encoding the high affinity antibodies can then be cloned into other lymphoid and non-lymphoid cells where the antibody can be expressed and from which the antibodies can be secreted.

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