公开(公告)号：US20140335557A1

公开(公告)日：2014-11-13

申请号：US14292535

申请日：2014-05-30

Applicant: RION CO., LTD.

Inventor： Kazuo ICHIJYO ,  Kazuma SEKIMOTO ,  Kousei SEKIGAWA ,  Yukihiro KIMOTO ,  Takayuki KOSAKA

IPC: G01J3/44

CPC classification number: G01J3/4406 ,  A61M1/16 ,  A61M1/1605 ,  A61M1/168 ,  A61M2205/3306 ,  A61M2205/52 ,  C02F1/008 ,  C02F1/44 ,  C02F2209/003 ,  C02F2209/005 ,  C02F2209/105 ,  G01N15/1459 ,  G01N21/6428 ,  G01N21/645 ,  G01N2015/1486

Abstract: A viable particle counting apparatus 77 includes devices 10, 70, 2. The device 10 radiates a light with a predetermined wavelength toward a liquid containing a detection target. The device 70 reduces a Raman-scattered light emitted from the liquid out of lights emitted due to an interaction of the light radiated by the device 10 with the target or the liquid and selects an autofluorescence light emitted from the target. The device 2 determines whether or not the target contained in the liquid is a viable particle, based on a light obtained after the Raman-scattered light is reduced by the device 70. The device 10 radiates the light with a wavelength that causes the autofluorescence light and the Raman-scattered light to be different in peak wavelength.

Abstract translation: 可行的颗粒计数装置77包括装置10,70,2。装置10将含有预定波长的光朝向包含检测对象的液体辐射。 装置70减少由于由装置10照射的光与目标物或液体的相互作用而发出的从发射的光中发出的拉曼散射光，并选择从目标射出的自发荧光。 装置2基于在通过装置70减少拉曼散射光之后获得的光来确定包含在液体中的靶是否是活的颗粒。装置10以引起自发荧光的波长辐射光 并且拉曼散射光的峰值波长不同。

公开(公告)号：US20150346077A1

公开(公告)日：2015-12-03

申请号：US14766578

申请日：2014-01-20

Applicant: RION CO., LTD.

Inventor： Kazuma SEKIMOTO

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1436 ,  G01N15/1459 ,  G01N21/6486 ,  G01N2015/0088 ,  G01N2015/1486

Abstract: This invention relates to a viable particle counting system. The system includes: a viable particle counting means that counts viable particles existing in a fluid by irradiating the fluid containing target particles to be detected with light at a predetermined wavelength, separating selectively autofluorescence or phosphorescence emitted from the target particles by the radiated light, receiving the separated autofluorescence or phosphorescence, and determining that the target particles are the viable particles according to the received autofluorescence or phosphorescence; and a pre-stage irradiation means that irradiates the fluid with ultraviolet light in advance before the viable particle counting means irradiates the fluid with the light at the predetermined wavelength.

Abstract translation: 本发明涉及一种可行的粒子计数系统。 该系统包括：可行的颗粒计数装置，通过用预定波长的光照射含有待检测目标颗粒的流体来计数流体中存在的颗粒，通过辐射光选择性地从目标颗粒发射的自发荧光或磷光分离，接收 分离的自发荧光或磷光，并且根据接收的自发荧光或磷光确定靶颗粒是活的颗粒; 以及在活粒子计数装置用预定波长的光照射流体之前，预先用紫外线照射流体的前级照射装置。

公开(公告)号：US20210063297A1

公开(公告)日：2021-03-04

申请号：US17003470

申请日：2020-08-26

Applicant: RION Co., Ltd.

Inventor： Kazuma SEKIMOTO

IPC: G01N15/06

Abstract: A microbial particle measuring apparatus includes: a light emitter configured to irradiate a fluid with light of a predetermined wavelength over a predetermined measurement time; a fluorescence receiver configured to selectively receive fluorescence emitted from particles contained in the fluid and output a signal having a magnitude corresponding to intensity of the fluorescence; a signal acquisition unit configured to obtain the signal output from the fluorescence receiver at regular intervals over the measurement time; and a determiner configured to calculate a slope of waveform of the signal obtained by the signal acquisition unit, and determine a concentration of microbial particles contained in the fluid using an attenuation amount of fluorescence intensity generated in a time period in which the slope in the measurement time is smaller than a predetermined value as an amount of attenuation derived from the microbial particles.

公开(公告)号：US20190310179A1

公开(公告)日：2019-10-10

申请号：US16461199

申请日：2017-11-07

Applicant: RION Co., Ltd.

Inventor： Kazuma SEKIMOTO

IPC: G01N15/14 ,  C12M1/00 ,  C12Q1/06 ,  C12M1/34

Abstract: A microbial particle is accurately counted in distinction from a non-microbial particle. A preceding-stage irradiation section 2 irradiates a sample as fluid with ultraviolet light at a preceding stage of a microbial particle counter 1. The ultraviolet light is ultraviolet light having a deep ultraviolet region, the ultraviolet light increasing the fluorescence intensity of a first autofluorescence substance in the microbial particle. The microbial particle counter 1 measures light intensity in a first wavelength range including the fluorescence wavelength of the first autofluorescence substance. In addition, the microbial particle counter 1 measures light intensity in a specific second wavelength range. Further, the microbial particle counter 1 counts the microbial particle in distinction from a non-microbial particle in the fluid based on the measured light intensity in the first wavelength range and the measured light intensity in the specific second wavelength range.

公开(公告)号：US20190003951A1

公开(公告)日：2019-01-03

申请号：US16064266

申请日：2016-12-05

Applicant: RION CO., LTD.

Inventor： Kazuma SEKIMOTO ,  Yuki OOHASHI ,  Takashi SANNOMIYA ,  Tsutomu NAKAJIMA ,  Miyuki FUKUDA ,  Masaru UENO ,  Yuuichi UEYA

IPC: G01N15/10 ,  G01N21/64

Abstract: The biological particle counter has a first circuit configured to generate a first signal corresponding to fluorescence from the biological particle in response to irradiated light. A measurement value obtainer is configured to obtain, from the biological particle counter, a voltage value of the first signal for a calibration particle obtained in such a manner that a fluorescence agent infiltrates into a surface of a polystyrene particle. A circuit adjuster is configured to use reference data indicating a correspondence relation between a fluorescence agent parameter value correlated to the content of the fluorescence agent and a reference voltage value of the first signal corresponding to the fluorescence agent parameter value, thereby specifying the reference voltage value of the first signal corresponding to the fluorescence agent parameter value for the calibration particle. The first circuit is adjusted such that the voltage value of the first signal for the calibration particle reaches the reference voltage value.

公开(公告)号：US20180058998A1

公开(公告)日：2018-03-01

申请号：US15681000

申请日：2017-08-18

Applicant: RION Co., Ltd.

Inventor： Kazuma SEKIMOTO

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/147 ,  G01N15/1459 ,  G01N15/1475 ,  G01N21/6428 ,  G01N2015/0088 ,  G01N2015/1486

Abstract: A microviable particle counting system includes: a microviable particle counting instrument configured to detect autofluorescence of a microviable particle in a sample as fluid, thereby counting the microviable particle in the sample; and a former-stage irradiator provided at a former stage of the microviable particle counting instrument to irradiate the sample with ultraviolet light. The ultraviolet light contains first ultraviolet light having such a wavelength that a carbon-carbon covalent bond is disconnected, and the first ultraviolet light has a wavelength shorter than 200 nm.