METHOD FOR EXTRACTING B-AMYLASES FROM A SOLUBLE FRACTION OF A STARCH PLANT AND IN THE PRESENCE OF A PROTEASE
    7.
    发明申请
    METHOD FOR EXTRACTING B-AMYLASES FROM A SOLUBLE FRACTION OF A STARCH PLANT AND IN THE PRESENCE OF A PROTEASE 审中-公开
    从淀粉植物的可溶性分解物中提取B-AMYLASES的方法和蛋白质的存在

    公开(公告)号:US20150184140A1

    公开(公告)日:2015-07-02

    申请号:US14419970

    申请日:2013-08-06

    申请人: ROQUETTE FRERES

    IPC分类号: C12N9/26

    摘要: An improved method for extracting β-amylases from a soluble fraction of a starch plant, includes a step of clarification by microfiltration and a step of concentration and purification by ultrafiltration. This method is characterized in that it uses a protease during the microfiltration step, making it possible to greatly reduce the fouling of the membranes used, which increases the production time before washing. Simultaneously, perfectly clear permeates are obtained, which assists with the subsequent ultrafiltration step and makes it possible to improve the transmission of β-amylase. Finally, the protease has no negative effect on the β-amylase activity.

    摘要翻译: 用于从淀粉植物的可溶性级分提取淀粉酶的改进方法包括通过微滤法澄清的步骤和通过超滤浓缩和纯化的步骤。 该方法的特征在于,在微滤步骤中使用蛋白酶,可以大大减少所使用的膜的结垢,这增加了洗涤前的生产时间。 同时,获得完全透明的渗透物,其有助于随后的超滤步骤,并且可以改善淀粉酶的传播。 最后,蛋白酶对淀粉酶活性没有负面影响。