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公开(公告)号：US08773759B2
公开(公告)日：2014-07-08
申请号：US13121886
申请日：2009-09-22
IPC分类号： G02B21/00
CPC分类号： G02B21/006 , G02B21/0068 , G02B21/241 , G02B2207/114
摘要： A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with a polarizing beam splitter for splitting the object light into two components that are oriented perpendicular to each other being disposed between the first and the second objective lens for light transmission.
摘要翻译： 一种具有用于调节焦距范围的装置的显微镜，包括用于在检测器的方向上传送被照射物体的物体的第一物镜，其中第二物镜沿检测器上游的光的方向设置， 第二物镜之后是可以在光轴方向上调节的第一反射镜，具有至少一个第二反射镜，用于在第二物镜的方向上从第一物镜透射光并从第二物镜传播光 所述检测器设置在所述光路中，所述第二反射镜是完全反射镜，或具有用于调节所述焦距的装置的显微镜，所述显微镜包括用于沿检测器的方向透射被照射物体的物体的第一物镜， 其中第二物镜沿着检测器上游的光的方向设置，该第二物镜后面是第一镜像 r可以在光轴的方向上调整，其中偏振分束器用于将物体光分成两个彼此垂直取向的分量，这两个分量被布置在第一和第二物镜之间用于透光。
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公开(公告)号：US20110176206A1
公开(公告)日：2011-07-21
申请号：US13121886
申请日：2009-09-22
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G02B21/00
CPC分类号： G02B21/006 , G02B21/0068 , G02B21/241 , G02B2207/114
摘要： A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with a polarizing beam splitter for splitting the object light into two components that are oriented perpendicular to each other being disposed between the first and the second objective lens for light transmission.
摘要翻译： 一种具有用于调节焦距范围的装置的显微镜，包括用于在检测器的方向上传送被照射物体的物体的第一物镜，其中第二物镜沿检测器上游的光的方向设置， 第二物镜之后是可以在光轴方向上调节的第一反射镜，具有至少一个第二反射镜，用于在第二物镜的方向上从第一物镜透射光并从第二物镜传播光 所述检测器设置在所述光路中，所述第二反射镜是完全反射镜，或具有用于调节所述焦距的装置的显微镜，所述显微镜包括用于沿检测器的方向透射被照射物体的物体的第一物镜， 其中第二物镜沿着检测器上游的光的方向设置，该第二物镜后面是第一镜像 r可以在光轴的方向上调整，其中偏振分束器用于将物体光分成两个彼此垂直取向的分量，这两个分量被布置在第一和第二物镜之间用于透光。
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公开(公告)号：US07605976B1
公开(公告)日：2009-10-20
申请号：US11416455
申请日：2006-05-03
IPC分类号： G02B21/06
CPC分类号： G01J3/4406 , G01J3/02 , G01J3/021 , G01J3/0224 , G01J3/0229 , G01J3/14 , G01N21/21 , G01N21/6445 , G01N21/6458 , G01N2021/6417 , G02B21/0068 , G02B21/0076 , G02B26/0816 , G02B27/143 , G02B27/283
摘要： Method and device for changing the illumination light and/or specimen light with respect to its spectral composition and/or intensity in an adjustable manner, wherein a spatial separation into the radiation components of different polarization is carried out with the first polarization means (Pol 1), a spectral spatial splitting of at least one radiation component is carried out with the first dispersion means (Disp1), and the polarization state of at least one part of the spectrally spatially split radiation component is changed, wherein a reflection of the illumination light and/or the detection light is carried out.
摘要翻译： 用于以可调节的方式改变照明光和/或样本光的方法和装置，其中利用第一偏振装置（Pol 1）执行不同偏振的辐射分量的空间分离 ），利用第一色散装置（Disp1）执行至少一个辐射分量的光谱空间分裂，并且改变光谱空间分裂的辐射分量的至少一部分的偏振状态，其中照明光的反射 和/或检测光。
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公开(公告)号：US20090250632A1
公开(公告)日：2009-10-08
申请号：US12444290
申请日：2007-10-02
IPC分类号： G01N21/64
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/0032 , G02B21/0072 , G02B21/0076
摘要： A method and arrangement for collimated microscopic imaging, including a first illumination of a sample in at least one region for exciting fluorescence, and a spatially resolving detection of the sample light by detector elements, the detection being associated with the region, wherein by means of a second illumination a sub-division of the region into separate fluorescent partial regions occurs, which are associated with the detector elements. The separation of the partial regions is carried out by the spatial separation of the fluorescent regions by means of intermediate regions having reduced fluorescence or no fluorescence, and/or by means of different spectral properties of the fluorescence from the partial regions.
摘要翻译： 一种用于准直显微镜成像的方法和装置，包括用于激发荧光的至少一个区域中的样品的第一照明，以及由检测器元件对样品光进行空间分辨检测，所述检测与所述区域相关联，其中通过 发生与检测器元件相关联的区域的分割成分离的荧光部分区域的第二照明。 部分区域的分离是通过荧光区域通过具有减少的荧光或无荧光的中间区域和/或通过来自部分区域的荧光的不同光谱性质的空间分离来进行的。
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5.发明申请Arrangement for the optical detection of light radiation which is excited and/or backscattered in a specimen with a double-objective arrangement 失效光学检测用于双目标布置的样品中被激发和/或反向散射的光辐射的布置公开(公告)号：US20050006597A1
公开(公告)日：2005-01-13
申请号：US10888896
申请日：2004-07-09
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
CPC分类号： G02B21/18 , G02B21/002
摘要： Arrangement and method for the optical detection of light radiation which is excited and/or backscattered in a specimen, wherein the illumination of the specimen and/or the detection of the specimen light is carried out by at least two objectives arranged on different sides of the specimen. The specimen illumination is focused in or in the vicinity of a pupil plane of the beam path between the specimen plane and the detection plane at least on one axis and an element for the spatial separation of the illumination light from the detection light are provided in this plane.
摘要翻译： 在样本中被激发和/或反向散射的光辐射的光学检测的布置和方法，其中样本的照明和/或样本光的检测通过布置在样品的不同侧面上的至少两个物体进行 标本。 样本照明至少在一个轴上聚焦在样本平面和检测平面之间的光束路径的光瞳平面附近或附近，并且在该位置处提供用于照明光与检测光的空间分离的元件 飞机
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公开(公告)号：US08908174B2
公开(公告)日：2014-12-09
申请号：US13121919
申请日：2009-09-22
CPC分类号： G01N21/6428 , G01N21/6458 , G01N2021/6417 , G02B21/0076 , G02B21/16
摘要： A microscope device which has a diffraction-limited resolution volume, with multiple dye molecules that can be switched between different states, at least one of which is fluorescent. The fluorescence is focused using an objective lens and is imaged onto a spatially resolving detector. In at least one portion of the sample, the dye molecules have a distribution density that is greater than the inverse of the diffraction-limited resolution volume. One or more light sources are provided for emitting a switching radiation in order to switch a first subset of the dye molecules in the sample, and for emitting an excitation radiation in order to excite the first subset of dye molecules. A phase mask which generates a light distribution having an at least partially limited local minimum radiation on the detector plane is provided in the beam path, preferably in the detection beam path.
摘要翻译： 具有衍射限制分辨率体积的显微镜装置，具有可以在不同状态之间切换的多个染料分子，其中至少一个是荧光的。 使用物镜聚焦荧光，并将其成像到空间分辨检测器上。 在样品的至少一部分中，染料分子的分布密度大于衍射极限分辨率体积的倒数。 提供一个或多个光源用于发射切换辐射，以便切换样品中的染料分子的第一子集，并且用于发射激发辐射以激发染料分子的第一子集。 优选在检测光束路径中，在光束路径中设置产生具有至少部分受限局部最小辐射的光分布的相位掩模。
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公开(公告)号：US08704196B2
公开(公告)日：2014-04-22
申请号：US13127427
申请日：2009-10-28
IPC分类号： G01J1/58
CPC分类号： G02B27/642 , G01N21/6458 , G02B21/0076 , G02B21/367 , G02B27/58
摘要： A method for generating an image of a sample by a microscopy method including varying local resolution, wherein at least two of the following microscopy methods are combined: laser scanning microscopy, a microscopy method wherein the sample is excited to luminescence by structured line or wide area illumination, and a first microscopy image is generated from the images thus obtained, having increased local resolution greater than the optical resolution of the image, a further microscopy method according to the PAL principle, by which a second microscopy image is generated, indicating geometric locations of marker molecules emitting luminescent radiation at an increased local resolution relative to the optical resolution, and a further microscopy method, wherein the sample is marked using marking molecules suitable for the STED, ESA, or RESOLFT technique, and a third microscopy image is generated of STED, ESA, or RESOLFT, wherein the obtained images are superimposed.
摘要翻译： 一种用于通过包括变化的局部分辨率的显微镜方法产生样品的图像的方法，其中组合以下显微镜方法中的至少两种：激光扫描显微镜，其中通过结构化线或广泛区域将样品激发至发光的显微镜方法 照明，并且从由此获得的图像产生第一显微镜图像，其具有大于图像的光学分辨率的增加的局部分辨率，根据PAL原理的另一显微镜方法，通过该方法生成第二显微镜图像，指示几何位置 相对于光学分辨率以增加的局部分辨率发射发光辐射的标记分子，以及另外的显微镜方法，其中使用适合于STED，ESA或RESOLFT技术的标记分子标记样品，并且产生第三显微镜图像 STED，ESA或RESOLFT，其中所获得的图像被叠加。
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公开(公告)号：US08610086B2
公开(公告)日：2013-12-17
申请号：US13131801
申请日：2009-11-14
IPC分类号： G01N21/64
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/361 , G02B21/367 , G02B27/58
摘要： Method for spatially high-resolution luminescence microscopy in which label molecules in a sample are activated to emit luminescence radiation comprising activating only a subset of the label molecules in the sample, wherein activated label molecules have a distance to the closest activated molecules that is greater or equal to a length which results from a predetermined optical resolution, detecting the luminescence radiation, generating a frame from the luminescence radiation, identifying the geometric locations of the label molecules with a spatial resolution increased above the predetermined optical resolution, repeating the steps and forming a combined image, and controlling the acquisition of the several frames by evaluating at least one of the frames or a group of the frames and modifying at least one variable for subsequent repetitions of the steps of generating frames for combining into an image.
摘要翻译： 用于空间高分辨率发光显微镜的方法，其中样品中的标记分子被激活以发射发光，包括仅激活样品中标记分子的一个子集，其中激活的标记分子与最接近的激活分子的距离更大或 等于由预定的光学分辨率产生的长度，检测发光辐射，从发光辐射产生帧，以高于预定的光学分辨率的空间分辨率识别标签分子的几何位置，重复步骤并形成 并且通过评估所述帧或一组帧中的至少一个并且修改至少一个变量来控制所述几帧的获取，以便后续重复生成用于组合成图像的帧的步骤。
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公开(公告)号：US08570625B2
公开(公告)日：2013-10-29
申请号：US12088557
申请日：2006-09-14
申请人： Michael Kempe , Ralf Wolleschensky
发明人： Michael Kempe , Ralf Wolleschensky
CPC分类号： G02B21/0032 , G02B21/006
摘要： A device for generating an image of an object is provided, comprising an illumination module, by means of which the object can be illuminated with a pattern whose phase is altered temporally, a recording module, by means of which a plurality of recordings of the object are carried out during the phase change of the pattern, and a processing module, which generates the image from the recordings, wherein the illumination module moves a light beam over the object and modulates its intensity synchronously with the movement such that the beam generates the pattern in scanning fashion.
摘要翻译： 提供了一种用于产生物体的图像的装置，包括照明模块，利用该照明模块可以利用其相位在时间上改变的图案来照亮物体;记录模块，借助于该物体的多个记录 在图案的相变期间进行，以及处理模块，其从记录产生图像，其中照明模块将光束移动到物体上并且与移动同步地调制其强度，使得光束产生图案 在扫描时尚。
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10.发明授权公开(公告)号：US08280131B2
公开(公告)日：2012-10-02
申请号：US12323123
申请日：2008-11-25
申请人： Michael Kempe , Ralf Wolleschensky
发明人： Michael Kempe , Ralf Wolleschensky
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0056
摘要： A configuration for the optical detection of a specimen, wherein the specimen or at least part of the specimen is scanned by means of linear illumination by scanning means, means for linear beam shaping of the illuminating light are provided, and the illuminating light has a preferably periodic structure in at least one spatial direction in that means for generating the structure are disposed in the illuminating beam path, light coming from the specimen is detected and images of the specimen are generated therefrom, at least one optical sectional image through the specimen and/or one image with increased resolution is/are calculated from the images, and means for generating the structure are disposed downstream of the scanning means in the direction of the illumination.
摘要翻译： 提供了一种用于通过扫描装置通过线性照明扫描样本或样本的至少一部分的样本的光学检测的结构，用于照明光的线性光束成形的装置，并且照明光具有优选的 在所述照明光束路径中设置用于产生所述结构的装置中的至少一个空间方向上的周期性结构，并且检测来自所述样本的光并且从其产生所述样本的图像，通过所述样本的至少一个光学截面图像和/ 或者从图像计算一个具有增加的分辨率的图像，并且用于产生该结构的装置设置在扫描装置的照明方向的下游。
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