公开(公告)号：US20060192955A1

公开(公告)日：2006-08-31

申请号：US11379972

申请日：2006-04-24

申请人： Ralph Jorgenson ,  David Basiji ,  William Ortyn

发明人： Ralph Jorgenson ,  David Basiji ,  William Ortyn

IPC分类号： G01J3/44 ,  G01N21/55

CPC分类号： G01N21/658 ,  B82Y15/00 ,  B82Y20/00 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/1434 ,  G01N15/147 ,  G01N21/554 ,  G01N21/6428 ,  G01N21/648 ,  G01N21/85 ,  G01N33/54346 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G01N2021/052 ,  G01N2021/058 ,  G01N2021/258 ,  G02B7/28 ,  G02B27/1013

摘要： A flow imaging system is used to implement surface plasmon resonance (SPR) detection to study bio-molecular interactions. The flow imaging system is used to capture SPR absorption spectra of large numbers of objects, where each object includes both a metal film capable of exhibiting SPR, and detecting molecules. Analyte molecules are added to a solution of such objects, and the result is introduced into the flow imaging system which collects full SPR spectral data from individual objects. The objects can be nanoparticles or larger particles that support metal island films. The SPR spectral data can be used to determine specificity, kinetics, affinity, and concentration with respect to the interactions between the detecting molecules and the analyte molecules.

摘要翻译： 流动成像系统用于实现表面等离子体共振（SPR）检测，以研究生物分子相互作用。 流动成像系统用于捕获大量物体的SPR吸收光谱，其中每个物体都包含能够显示SPR的金属膜和检测分子。 将分析物分子加入到这些物体的溶液中，并将结果引入到从各个物体收集完整的SPR光谱数据的流动成像系统中。 物体可以是支撑金属岛膜的纳米颗粒或更大的颗粒。 SPR光谱数据可用于确定相对于检测分子和分析物分子之间的相互作用的特异性，动力学，亲和力和浓度。

公开(公告)号：US5991048A

公开(公告)日：1999-11-23

申请号：US738360

申请日：1996-10-25

申请人： Scott Karlson ,  Sinclair S. Yee ,  Kyle Johnston ,  Ralph Jorgenson

发明人： Scott Karlson ,  Sinclair S. Yee ,  Kyle Johnston ,  Ralph Jorgenson

IPC分类号： G01N21/55 ,  G01N21/77 ,  G02B6/10 ,  G02B6/24 ,  G02B6/28

CPC分类号： G01N21/553 ,  G02B6/10 ,  G02B6/24 ,  G02B6/2826

摘要： The present invention provides SPR sensors in which the sensing element is a planar lightpipe. The sensors of this invention include configurations which employ multiwavelength light incident on the SPR sensing area at a single angle or at a range of angles. Sensors of this invention also include configurations that employ monochromatic light at a range of angles. Many of the configurations of the SPR lightpipe sensors of this invention involve imaging of input light through the lightpipe. In one embodiment, the invention provides a first order SPR sensor system in which the sensing element is a planar lightpipe. Light coupled into the lightpipe reflects off an SPR sensing area positioned on an external surface planar surface of the lightpipe. Multiwavelength light that is coupled into the lightpipe input face at a range of angles propagates through the lightpipe by total internal reflection (TIR), making multiple reflections, and exits in a series of angular bands each containing spectral information (including SPR features)for a small range of incidence angles. A detector or detectors are positioned to measure the reflection spectrum, including any surface plasmon resonance feature, of one or preferably more than one of the angular bands exiting the lightpipe. SPR sensor configurations include those that have multiple sensing channels and those which can be multiplexed. This invention provides SPR sensors with planar lightpipe sensing elements and method of detecting analytes in samples using these sensors. The invention also provides planar lightpipe that have a plurality of SPR sensing layers on a planar surface.

摘要翻译： 本发明提供了SPR传感器，其中感测元件是平面光管。 本发明的传感器包括采用在单个角度或角度范围内入射到SPR感测区域的多波长光的配置。 本发明的传感器还包括采用角度范围内的单色光的构造。 本发明的SPR光管传感器的许多配置涉及通过光管对输入光的成像。 在一个实施例中，本发明提供了第一级SPR传感器系统，其中感测元件是平面光管。 耦合到光管中的光从位于光管的外表面平面上的SPR感测区域反射出来。 以一定范围的角度耦合到光管输入面中的多波长光通过全内反射（TIR）传播通过光管，进行多次反射，并在一系列角频带中退出，每个角带包含光谱信息（包括SPR特征），用于 入射角范围小。 定位检测器或检测器以测量离开光管的一个或优选多于一个角带的反射光谱，包括任何表面等离子体共振特征。 SPR传感器配置包括具有多个感测通道的那些，以及可复用的那些。 本发明提供具有平面光管感测元件的SPR传感器以及使用这些传感器检测样品中的分析物的方法。 本发明还提供了在平坦表面上具有多个SPR感测层的平面光管。