利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

19 条记录 (耗时 0.038 秒)

    DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY
    1.
    发明申请
    DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY 有权
    使用成像流程细胞计数检测循环肿瘤细胞

    公开(公告)号：US20120148142A1

    公开(公告)日：2012-06-14

    申请号：US13396333

    申请日：2012-02-14

    申请人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    发明人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    IPC分类号： G06K9/62

    CPC分类号： G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/505 ,  G01N2021/6441

    摘要： Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.

    摘要翻译： 作为细胞分析仪，使用从流动细胞的多模式图像得到的光度和形态测定特征，以确定对应于癌细胞或癌前细胞的标志物是否存在于成像细胞群体中。 成像系统同时获取通过成像系统的视野的每个单元的多个图像。 获取多个不同的图像（即，亮场，暗场和荧光图像）有助于确定不同的形态和形态测量参数。 同时获取多个图像使得能够快速成像相对较大数量的细胞，从而可以检测大量细胞中相对少数量的癌细胞。 首先，将已知的癌细胞成像以使得能够鉴定标记。 然后，可以包含癌细胞的样品被成像以确定标记物是否存在。

    Auto focus for a flow imagin system
    3.
    发明申请
    Auto focus for a flow imagin system 有权
    自动对焦一个流量想象系统

    公开(公告)号：US20050127271A1

    公开(公告)日：2005-06-16

    申请号：US11045675

    申请日：2005-01-28

    申请人： William Ortyn ,  Michael Seo ,  David Basiji ,  Keith Frost ,  David Perry

    发明人： William Ortyn ,  Michael Seo ,  David Basiji ,  Keith Frost ,  David Perry

    IPC分类号： G01N15/10 ,  G01N15/14 ,  G02B27/00 ,  G06T7/20 ,  G01N21/00 ,  G01N15/06 ,  G02B7/04

    CPC分类号： G06T7/20 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/1018 ,  G01N2015/1443 ,  G01N2015/1447 ,  G01N2015/1452 ,  G01N2015/1497 ,  G02B27/0012 ,  G06K9/00134

    摘要： A pair of optical gratings are used to modulate light from an object, and the modulated light from either grating is used to determine the velocity of the object. Each optical grating is offset from a reference focal point by the same distance, one grating being offset in a positive direction, the other in a negative direction. Signals produced in response to the modulated light can be processed to determine a direction in which a primary collection lens should be moved in order to improve a focus of the imaging system on the object. The lens is moved incrementally in the direction so determined, and the process is repeated until an optimal focus is achieved. In a preferred embodiment, the signals are weighted, so that the optical grating disposed closest to the optimal focus position contributes the most to velocity detection.

    摘要翻译： 一对光栅用于调制来自物体的光，并且来自任一光栅的调制光用于确定物体的速度。 每个光栅从参考焦点偏移相同的距离，一个光栅在正方向偏移，另一个光栅在负方向上偏移。 可以处理响应于调制光产生的信号以确定主收集透镜应该移动的方向，以便改善成像系统对物体的焦点。 透镜在如此确定的方向上逐渐移动，并重复该过程，直到达到最佳聚焦。 在优选实施例中，对信号进行加权，使得最靠近最佳聚焦位置布置的光栅最有助于速度检测。

    Detection of circulating tumor cells using imaging flow cytometry
    4.
    发明授权
    Detection of circulating tumor cells using imaging flow cytometry 有权
    使用成像流式细胞术检测循环肿瘤细胞

    公开(公告)号：US08548219B2

    公开(公告)日：2013-10-01

    申请号：US13396333

    申请日：2012-02-14

    申请人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    发明人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    IPC分类号： G06K9/00 ,  G01J3/28

    CPC分类号： G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/505 ,  G01N2021/6441

    摘要： Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.

    摘要翻译： 作为细胞分析仪，使用从流动细胞的多模式图像得到的光度和形态测定特征，以确定对应于癌细胞或癌前细胞的标志物是否存在于成像细胞群体中。 成像系统同时获取通过成像系统的视野的每个单元的多个图像。 获取多个不同的图像（即，亮场，暗场和荧光图像）有助于确定不同的形态和形态测量参数。 同时获取多个图像使得能够快速成像相对较大数量的细胞，从而可以检测大量细胞中相对少数量的癌细胞。 首先，将已知的癌细胞成像以使得能够鉴定标记。 然后，可以包含癌细胞的样品被成像以确定标记物是否存在。

    Detection of circulating tumor cells using imaging flow cytometry
    5.
    发明授权
    Detection of circulating tumor cells using imaging flow cytometry 有权
    使用成像流式细胞术检测循环肿瘤细胞

    公开(公告)号：US08131053B2

    公开(公告)日：2012-03-06

    申请号：US12181062

    申请日：2008-07-28

    申请人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    发明人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    IPC分类号： G06K9/00 ,  G01J3/28

    CPC分类号： G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/505 ,  G01N2021/6441

    摘要： Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.

    摘要翻译： 作为细胞分析仪，使用从流中的细胞的多模式图像得到的光度和形态测定特征，以确定对应于癌细胞或癌前细胞的标志物是否存在于成像细胞群体中。 成像系统同时获取通过成像系统的视野的每个单元的多个图像。 获取多个不同的图像（即，亮场，暗场和荧光图像）有助于确定不同的形态和形态测量参数。 同时获取多个图像使得能够快速成像相对较大数量的细胞，从而可以检测大量细胞中相对少数量的癌细胞。 首先，将已知的癌细胞成像以使得能够鉴定标记。 然后，可以包含癌细胞的样品被成像以确定标记物是否存在。

    Extended depth of field imaging for high speed object analysis
    6.
    发明授权
    Extended depth of field imaging for high speed object analysis 有权
    用于高速物体分析的扩展景深成像

    公开(公告)号：US08009189B2

    公开(公告)日：2011-08-30

    申请号：US12132394

    申请日：2008-06-03

    申请人： William Ortyn ,  David Basiji ,  Keith Frost ,  Luchuan Liang ,  Richard Bauer ,  Brian Hall ,  David Perry

    发明人： William Ortyn ,  David Basiji ,  Keith Frost ,  Luchuan Liang ,  Richard Bauer ,  Brian Hall ,  David Perry

    IPC分类号： G01J3/28 ,  G01J3/51

    CPC分类号： G02B27/0075 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1452 ,  G02B21/365 ,  G02B27/0012

    摘要： A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

    摘要翻译： 改进了高速，高分辨率流动成像系统，以实现扩展的景深成像。 光学失真元件被引入到流动成像系统中。 来自诸如单元的物体的光被失真元件扭曲，使得成像系统的点扩散函数（PSF）在扩展的景深范围内是不变的。 失真光被光谱分散，并且分散的光被用于同时产生多个图像。 检测图像，并且使用图像处理来通过补偿失真来增强检测到的图像，以实现对象的扩展景深图像。 后图像处理优选地涉及去卷积，并且需要由光学失真元件修改的成像系统的PSF的知识。

    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS
    7.
    发明申请
    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS 有权
    用于高速物体分析的现场成像的扩展深度

    公开(公告)号：US20080234984A1

    公开(公告)日：2008-09-25

    申请号：US12132394

    申请日：2008-06-03

    申请人： William Ortyn ,  David Basiji ,  Keith Frost ,  Luchuan Liang ,  Richard Bauer ,  Brian Hall ,  David Perry

    发明人： William Ortyn ,  David Basiji ,  Keith Frost ,  Luchuan Liang ,  Richard Bauer ,  Brian Hall ,  David Perry

    IPC分类号： G06F15/00

    CPC分类号： G02B27/0075 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1452 ,  G02B21/365 ,  G02B27/0012

    摘要： A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

    摘要翻译： 改进了高速，高分辨率流动成像系统，以实现扩展的景深成像。 光学失真元件被引入到流动成像系统中。 来自诸如单元的物体的光被失真元件扭曲，使得成像系统的点扩散函数（PSF）在扩展的景深范围内是不变的。 失真光被光谱分散，并且分散的光被用于同时产生多个图像。 检测图像，并且使用图像处理来通过补偿失真来增强检测到的图像，以实现对象的扩展景深图像。 后图像处理优选地涉及去卷积，并且需要由光学失真元件修改的成像系统的PSF的知识。

    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS
    8.
    发明申请
    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS 有权
    用于高速物体分析的现场成像的扩展深度

    公开(公告)号：US20070146873A1

    公开(公告)日：2007-06-28

    申请号：US11609269

    申请日：2006-12-11

    申请人： William Ortyn ,  David Basiji ,  Keith Frost ,  Luchuan Liang ,  Richard Bauer ,  Brian Hall ,  David Perry

    发明人： William Ortyn ,  David Basiji ,  Keith Frost ,  Luchuan Liang ,  Richard Bauer ,  Brian Hall ,  David Perry

    IPC分类号： G02B21/06

    CPC分类号： G02B27/0075 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1452 ,  G02B21/365 ,  G02B27/0012

    摘要： A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

    摘要翻译： 改进了高速，高分辨率流动成像系统，以实现扩展的景深成像。 光学失真元件被引入到流动成像系统中。 来自诸如单元的物体的光被失真元件扭曲，使得成像系统的点扩散函数（PSF）在扩展的景深范围内是不变的。 失真光被光谱分散，并且分散的光被用于同时产生多个图像。 检测图像，并且使用图像处理来通过补偿失真来增强检测到的图像，以实现对象的扩展景深图像。 后图像处理优选地涉及去卷积，并且需要由光学失真元件修改的成像系统的PSF的知识。