公开(公告)号：US07338799B2

公开(公告)日：2008-03-04

申请号：US10724806

申请日：2003-12-01

申请人： Randy D. Blakely ,  Subramaniam Apparsundaram ,  Shawn Ferguson

发明人： Randy D. Blakely ,  Subramaniam Apparsundaram ,  Shawn Ferguson

IPC分类号： C07H21/04 ,  C12N5/00 ,  C12N15/00 ,  C12N7/01 ,  C07K14/47

CPC分类号： C07K14/47 ,  A01K2217/05 ,  A61K48/00

摘要： Human high affinity choline transporter (CHT) cDNA was cloned from spinal cord and the primary structure and chromosomal localization have been determined. Mouse high affinity CHT cDNA was also cloned and characterized. An isolated polynucleotide, an isolated polypeptide, a recombinant host cell, a recombinant vector, a purified protein, an antibody, a nucleic acid detection kit, a method for screening cholinergic therapeutics, a method of treating a patient, a method of gene therapy and transgenic CHT mice are discussed.

摘要翻译： 从脊髓中克隆人高亲和力胆碱转运蛋白（CHT）cDNA，并确定了一级结构和染色体定位。 还克隆和表征小鼠高亲和力的CHT cDNA。 分离的多核苷酸，分离的多肽，重组宿主细胞，重组载体，纯化蛋白，抗体，核酸检测试剂盒，用于筛选胆碱能治疗剂的方法，治疗患者的方法，基因治疗方法和 讨论了转基因CHT小鼠。