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公开(公告)号：US4804628A
公开(公告)日：1989-02-14
申请号：US88463
申请日：1987-08-19
摘要： An improved cell culturing device includes a hollow fiber cartridge having a shell and a plurality of capillaries extending through the shell with at least some of the capillaries having selectively permeable walls. A cell culturing space is located between the shell and capillaries. The improvement includes a chamber containing a second medium supply fluidly connected to the cell culturing space. A pressurizing system pressurizes the medium within the chamber to a level higher than the level of medium flowing through the lumen of the capillaries. A valving mechanism alternatively and selectively restricts flow of medium between the chamber and the cell culturing space through first and second conduits such that circulation is effected in the cell culturing space.
摘要翻译： 一种改进的细胞培养装置包括具有壳体和多个毛细管的中空纤维筒，所述毛细管延伸穿过壳体，其中至少一些毛细管具有选择性渗透的壁。 细胞培养空间位于壳和毛细血管之间。 该改进包括包含流体连接到细胞培养空间的第二介质供应的腔室。 加压系统将腔室内的介质加压到高于流过毛细管内腔的介质水平的水平。 瓣膜机构可选地且选择性地通过第一和第二导管限制室和细胞培养空间之间的介质流动，使得在细胞培养空间中进行循环。
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公开(公告)号：US08714598B2
公开(公告)日：2014-05-06
申请号：US13307777
申请日：2011-11-30
IPC分类号： F16L27/087 , F16L17/00
CPC分类号： F16L27/0828 , F16L17/025
摘要： A hydraulic fluid coupling for attaching two hydraulic hoses that comprises an inline swivel joint to prevent twisting of the hoses. The coupling comprises a first and second coupling portion, with the second coupling portion rotatable relative to the first coupling portion. There are two bearings and a ring shaped piston that slides toward the second bearing in response to fluid pressure inside the coupling. Engagement of the piston with the second bearing urges the second coupling portion towards the first coupling portion, against the action of fluid pressure between the first and second couplings. This reduces the load on the first bearing and transfers some of that load to the second bearing, thereby making it easier to rotate the second coupling portion.
摘要翻译： 用于连接两个液压软管的液压流体联轴器，其包括在线旋转接头以防止软管的扭转。 联接器包括第一和第二联接部分，第二联接部分可相对于第一联接部分旋转。 有两个轴承和环形活塞，以响应于联轴器内的流体压力而朝向第二轴承滑动。 活塞与第二轴承的接合促使第二联接部分朝着第一联接部分抵抗第一和第二联接件之间的流体压力的作用。 这减小了第一轴承上的负载并且将一些负载转移到第二轴承，从而使得更容易旋转第二联接部分。
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公开(公告)号：US5590482A
公开(公告)日：1997-01-07
申请号：US495340
申请日：1995-06-27
CPC分类号： E02F3/962 , E02F3/404 , Y10S37/903
摘要： An earthen material excavator includes, a) a base undercarriage comprising running gear to move the excavator over the ground; b) a slewing platform; c) a plurality of booms operatively connected to one another, one of the booms being operatively connected with the slewing platform; and d) a digging bucket apparatus mounted to another of the booms, the bucket apparatus including, i) a rigid digging assembly having a fore earth working end and an aft non-earth-working end, the digging assembly comprising an internal throat positioned between the assembly fore and aft ends; and ii) a substantially rigid earthen material container removably mounted relative to the aft end of the rigid digging assembly, the rigid earthen container being mounted in communication with the throat to receive earthen material transferred from the rigid digging assembly through the throat.
摘要翻译： 土石料挖掘机包括：a）底座底盘，包括行走齿轮，使挖掘机在地面上移动; b）回转平台; c）多个可操作地连接的起重臂，其中一个悬臂与回转平台可操作地连接; 以及d）安装在另一个悬臂上的挖斗装置，所述铲斗装置包括：i）刚性挖掘组件，具有前土工作端和后非工作端，所述挖掘组件包括位于 前后组合; 以及ii）相对于刚性挖掘组件的后端可移除地安装的基本上刚性的土层容器，所述刚性土容器安装成与所述喉部连通以接收从所述刚性挖掘组件通过所述喉部传送的土壤材料。
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公开(公告)号：US20120139233A1
公开(公告)日：2012-06-07
申请号：US13307777
申请日：2011-11-30
IPC分类号： F16L27/00
CPC分类号： F16L27/0828 , F16L17/025
摘要： A hydraulic fluid coupling for attaching two hydraulic hoses that comprises an inline swivel joint to prevent twisting of the hoses. The coupling comprises a first and second coupling portion, with the second coupling portion rotatable relative to the first coupling portion. There are two bearings and a ring shaped piston that slides toward the second bearing in response to fluid pressure inside the coupling. Engagement of the piston with the second bearing urges the second coupling portion towards the first coupling portion, against the action of fluid pressure between the first and second couplings. This reduces the load on the first bearing and transfers some of that load to the second bearing, thereby making it easier to rotate the second coupling portion.
摘要翻译： 用于连接两个液压软管的液压流体联轴器，其包括在线旋转接头以防止软管的扭转。 联接器包括第一和第二联接部分，第二联接部分可相对于第一联接部分旋转。 有两个轴承和环形活塞，以响应于联轴器内的流体压力而朝向第二轴承滑动。 活塞与第二轴承的接合促使第二联接部分朝着第一联接部分抵抗第一和第二联接件之间的流体压力的作用。 这减小了第一轴承上的负载并且将一些负载转移到第二轴承，从而使得更容易旋转第二联接部分。
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公开(公告)号：USD372886S
公开(公告)日：1996-08-20
申请号：US45277
申请日：1995-10-16
申请人： Robert D. Walker
设计人： Robert D. Walker
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6.发明授权Methods for detecting and enumerating Campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains 失效在环境样品中检测和计数空肠弯曲杆菌并鉴定抗生素抗性菌株的方法公开(公告)号：US06355435B1
公开(公告)日：2002-03-12
申请号：US09656286
申请日：2000-09-06
申请人： David L. Wilson , John E. Linz , John B. Kaneene , Linda S. Mansfield , Robert D. Walker , Thomas C. Newman
发明人： David L. Wilson , John E. Linz , John B. Kaneene , Linda S. Mansfield , Robert D. Walker , Thomas C. Newman
IPC分类号： C12Q168
CPC分类号： C12Q1/689 , C07H21/00 , C12Q2600/156 , Y10S435/80
摘要： The present invention provides a process for detecting and enumerating Campylobacter jejuni in an environmental sample. The present invention further provides a process which can distinguish antibiotic resistant strains of Campylobacter jejuni from wild-type strains, in particular, antibiotic-resistant strains resistant to high levels of ciprofloxacin. Both processes use PCR primers which flank a target sequence unique to Campylobacter jejuni in combination with one or more dual-labeled oligonucleotide probes complementary to the target sequence wherein the dual-labeled probes enable detection of PCR amplification by fluorescence detection means.
摘要翻译： 本发明提供了一种在环境样品中检测和计数空肠弯曲杆菌的方法。 本发明还提供了一种能够将空肠弯曲杆菌的抗生素抗性菌株与野生型菌株，特别是对高水平环丙沙星耐药的抗生素抗性菌株区分开的方法。 两种方法使用侧翼于空肠弯曲杆菌独特的靶序列的PCR引物与与靶序列互补的一个或多个双标记寡核苷酸探针组合，其中双标记探针能够通过荧光检测手段检测PCR扩增。
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公开(公告)号：USD333760S
公开(公告)日：1993-03-09
申请号：US731938
申请日：1991-07-18
申请人： Robert D. Walker
设计人： Robert D. Walker
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8.发明授权Apparatus for administering a controlled dosage of a chemical substance having an improved culture chamber 失效用于施用受控剂量的具有改善的培养室的化学物质的装置
公开(公告)号：US4618586A
公开(公告)日：1986-10-21
申请号：US483284
申请日：1983-04-08
申请人： Robert D. Walker
发明人： Robert D. Walker
CPC分类号： C12M35/08 , A01N1/0242 , A01N1/0247 , C12M41/48 , G05D11/138
摘要： An apparatus delivers a dosage of a chemical stimuli or drug in such a controlled manner that the concentration at the point of delivery is known at each and every point in time. The apparatus includes a plurality of vessels with each vessel containing a different concentration of the drug or chemical stimuli. A valve has a plurality of inlets and a single outlet wherein each inlet is connected to a corresponding vessel. An improved culture chamber is fluidly connected to the outlet of the valve and is used to develop the desired concentration. A first pump is positioned preferably between the valve and the chamber to provide a transport force for delivering the chemical stimuli or drug to the chamber. A suction tube is positioned proximate the top of the chamber and has a bottom open end which defines the level of liquid within the chamber. The suction tube thereby keeps the volume within the chamber constant. A second pump provides a transport force for removing excess liquid from the chamber. The valve, the first pump and the second pump are controlled by a computer control system. The computer control system determines which inlet of the valve is fluidly connected to the outlet to provide a preselected concentration of the chemical substance to the chamber with the pump being controlled by the computer to provide a predetermined flow rate of the medium to the chamber. The second pump is controlled by the computer removing excess volume from the chamber to insure that the volume within the chamber is kept constant. The control of the valve, the first and second pumps, are such that the concentration of the chemical stimuli or drug in the chamber is controlled and known at each and every point in time.
摘要翻译： 一种装置以这样的受控方式递送化学刺激或药物的剂量，使得在每个时间点已知递送点处的浓度。 该装置包括多个容器，每个容器含有不同浓度的药物或化学刺激物。 阀具有多个入口和单个出口，其中每个入口连接到相应的容器。 改进的培养室流体地连接到阀的出口并用于培养所需的浓度。 第一泵优选地定位在阀和腔室之间，以提供用于将化学刺激或药物输送到腔室的输送力。 抽吸管定位在室的顶部附近，并且具有限定腔室内的液体水平的底部开口端。 因此，吸入管将容积保持恒定。 第二泵提供用于从腔室去除多余液体的输送力。 阀，第一泵和第二泵由计算机控制系统控制。 计算机控制系统确定阀的哪个入口流体地连接到出口，以向泵提供化学物质的预选浓度，其中泵由计算机控制以提供介质到腔室的预定流速。 第二个泵由计算机控制，从室中移除多余的体积，以确保室内的体积保持恒定。 阀，第一和第二泵的控制使得腔室中的化学刺激或药物的浓度在每个时间点被控制和已知。
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公开(公告)号：US3976279A
公开(公告)日：1976-08-24
申请号：US412782
申请日：1973-11-05
申请人： Robert D. Walker
发明人： Robert D. Walker
IPC分类号： F16K31/50
CPC分类号： F16K31/508
摘要： A valve is disclosed that has an upper stem and a lower stem that are received in a gland and connected together by a threaded connection. The gland has an axial slot and the lower valve stem has a diametrically extending pin that extends into the slot so that when the upper stem is rotated, the lower stem moves axially into engagement with a valve seat thereby eliminating a rotation of the lower stem which insures against the stem rotating on the valve seat and prevents galling and reduces backlash between the upper stem and the lower stem to being almost non-existent without requiring periodic adjustment to compensate for wear.
摘要翻译： 公开了一种具有上杆和下杆的阀，其被接收在密封件中并通过螺纹连接连接在一起。 压盖具有轴向槽，下阀杆具有延伸到槽中的直径延伸的销，使得当上杆旋转时，下杆轴向移动与阀座接合，从而消除下杆的旋转， 确保在阀座上旋转的阀杆并且防止磨损并且减小上阀杆和下阀杆之间的间隙几乎不存在，而不需要周期性的调节以补偿磨损。
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